Cargando…
Fungitoxicity against Botrytis cinerea of a Flavonoid Isolated from Pseudognaphalium robustum
The fungotoxicity against Botrytis cinerea of a flavonoid isolated from Pseudognaphalium robustum was analyzed. Two absorption column chromatographies and one semipreparative thin layer chromatography were used to purify the active flavonoid. It was determined, by (1)H-NMR spectroscopy and co-elutio...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6263258/ http://dx.doi.org/10.3390/molecules16053885 |
_version_ | 1783375253191262208 |
---|---|
author | Cotoras, Milena Mendoza, Leonora Muñoz, Alexis Yáñez, Karen Castro, Paulo Aguirre, María |
author_facet | Cotoras, Milena Mendoza, Leonora Muñoz, Alexis Yáñez, Karen Castro, Paulo Aguirre, María |
author_sort | Cotoras, Milena |
collection | PubMed |
description | The fungotoxicity against Botrytis cinerea of a flavonoid isolated from Pseudognaphalium robustum was analyzed. Two absorption column chromatographies and one semipreparative thin layer chromatography were used to purify the active flavonoid. It was determined, by (1)H-NMR spectroscopy and co-elution with standards in HPLC, that this compound was 5,7-dihydroxy-3,8-dimethoxyflavone (gnaphaliin A). To determine the fungitoxicity of the purified compound, the effect on in vitro mycelial growth and conidial germination was studied. The compound concentration that reduced mycelial growth by 50% was 45.5 μg/mL. This compound also partially affected conidial germination of B. cinerea, reduced oxygen consumption by germinating conidia and affected the integrity of plasma membrane. Finally, using cyclic voltammetry, it was shown that the purified flavone had a pro-oxidant effect. |
format | Online Article Text |
id | pubmed-6263258 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62632582018-12-10 Fungitoxicity against Botrytis cinerea of a Flavonoid Isolated from Pseudognaphalium robustum Cotoras, Milena Mendoza, Leonora Muñoz, Alexis Yáñez, Karen Castro, Paulo Aguirre, María Molecules Article The fungotoxicity against Botrytis cinerea of a flavonoid isolated from Pseudognaphalium robustum was analyzed. Two absorption column chromatographies and one semipreparative thin layer chromatography were used to purify the active flavonoid. It was determined, by (1)H-NMR spectroscopy and co-elution with standards in HPLC, that this compound was 5,7-dihydroxy-3,8-dimethoxyflavone (gnaphaliin A). To determine the fungitoxicity of the purified compound, the effect on in vitro mycelial growth and conidial germination was studied. The compound concentration that reduced mycelial growth by 50% was 45.5 μg/mL. This compound also partially affected conidial germination of B. cinerea, reduced oxygen consumption by germinating conidia and affected the integrity of plasma membrane. Finally, using cyclic voltammetry, it was shown that the purified flavone had a pro-oxidant effect. MDPI 2011-05-09 /pmc/articles/PMC6263258/ http://dx.doi.org/10.3390/molecules16053885 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Cotoras, Milena Mendoza, Leonora Muñoz, Alexis Yáñez, Karen Castro, Paulo Aguirre, María Fungitoxicity against Botrytis cinerea of a Flavonoid Isolated from Pseudognaphalium robustum |
title | Fungitoxicity against Botrytis cinerea of a Flavonoid Isolated from Pseudognaphalium robustum |
title_full | Fungitoxicity against Botrytis cinerea of a Flavonoid Isolated from Pseudognaphalium robustum |
title_fullStr | Fungitoxicity against Botrytis cinerea of a Flavonoid Isolated from Pseudognaphalium robustum |
title_full_unstemmed | Fungitoxicity against Botrytis cinerea of a Flavonoid Isolated from Pseudognaphalium robustum |
title_short | Fungitoxicity against Botrytis cinerea of a Flavonoid Isolated from Pseudognaphalium robustum |
title_sort | fungitoxicity against botrytis cinerea of a flavonoid isolated from pseudognaphalium robustum |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6263258/ http://dx.doi.org/10.3390/molecules16053885 |
work_keys_str_mv | AT cotorasmilena fungitoxicityagainstbotrytiscinereaofaflavonoidisolatedfrompseudognaphaliumrobustum AT mendozaleonora fungitoxicityagainstbotrytiscinereaofaflavonoidisolatedfrompseudognaphaliumrobustum AT munozalexis fungitoxicityagainstbotrytiscinereaofaflavonoidisolatedfrompseudognaphaliumrobustum AT yanezkaren fungitoxicityagainstbotrytiscinereaofaflavonoidisolatedfrompseudognaphaliumrobustum AT castropaulo fungitoxicityagainstbotrytiscinereaofaflavonoidisolatedfrompseudognaphaliumrobustum AT aguirremaria fungitoxicityagainstbotrytiscinereaofaflavonoidisolatedfrompseudognaphaliumrobustum |