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Fungitoxicity against Botrytis cinerea of a Flavonoid Isolated from Pseudognaphalium robustum

The fungotoxicity against Botrytis cinerea of a flavonoid isolated from Pseudognaphalium robustum was analyzed. Two absorption column chromatographies and one semipreparative thin layer chromatography were used to purify the active flavonoid. It was determined, by (1)H-NMR spectroscopy and co-elutio...

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Autores principales: Cotoras, Milena, Mendoza, Leonora, Muñoz, Alexis, Yáñez, Karen, Castro, Paulo, Aguirre, María
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6263258/
http://dx.doi.org/10.3390/molecules16053885
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author Cotoras, Milena
Mendoza, Leonora
Muñoz, Alexis
Yáñez, Karen
Castro, Paulo
Aguirre, María
author_facet Cotoras, Milena
Mendoza, Leonora
Muñoz, Alexis
Yáñez, Karen
Castro, Paulo
Aguirre, María
author_sort Cotoras, Milena
collection PubMed
description The fungotoxicity against Botrytis cinerea of a flavonoid isolated from Pseudognaphalium robustum was analyzed. Two absorption column chromatographies and one semipreparative thin layer chromatography were used to purify the active flavonoid. It was determined, by (1)H-NMR spectroscopy and co-elution with standards in HPLC, that this compound was 5,7-dihydroxy-3,8-dimethoxyflavone (gnaphaliin A). To determine the fungitoxicity of the purified compound, the effect on in vitro mycelial growth and conidial germination was studied. The compound concentration that reduced mycelial growth by 50% was 45.5 μg/mL. This compound also partially affected conidial germination of B. cinerea, reduced oxygen consumption by germinating conidia and affected the integrity of plasma membrane. Finally, using cyclic voltammetry, it was shown that the purified flavone had a pro-oxidant effect.
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spelling pubmed-62632582018-12-10 Fungitoxicity against Botrytis cinerea of a Flavonoid Isolated from Pseudognaphalium robustum Cotoras, Milena Mendoza, Leonora Muñoz, Alexis Yáñez, Karen Castro, Paulo Aguirre, María Molecules Article The fungotoxicity against Botrytis cinerea of a flavonoid isolated from Pseudognaphalium robustum was analyzed. Two absorption column chromatographies and one semipreparative thin layer chromatography were used to purify the active flavonoid. It was determined, by (1)H-NMR spectroscopy and co-elution with standards in HPLC, that this compound was 5,7-dihydroxy-3,8-dimethoxyflavone (gnaphaliin A). To determine the fungitoxicity of the purified compound, the effect on in vitro mycelial growth and conidial germination was studied. The compound concentration that reduced mycelial growth by 50% was 45.5 μg/mL. This compound also partially affected conidial germination of B. cinerea, reduced oxygen consumption by germinating conidia and affected the integrity of plasma membrane. Finally, using cyclic voltammetry, it was shown that the purified flavone had a pro-oxidant effect. MDPI 2011-05-09 /pmc/articles/PMC6263258/ http://dx.doi.org/10.3390/molecules16053885 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Cotoras, Milena
Mendoza, Leonora
Muñoz, Alexis
Yáñez, Karen
Castro, Paulo
Aguirre, María
Fungitoxicity against Botrytis cinerea of a Flavonoid Isolated from Pseudognaphalium robustum
title Fungitoxicity against Botrytis cinerea of a Flavonoid Isolated from Pseudognaphalium robustum
title_full Fungitoxicity against Botrytis cinerea of a Flavonoid Isolated from Pseudognaphalium robustum
title_fullStr Fungitoxicity against Botrytis cinerea of a Flavonoid Isolated from Pseudognaphalium robustum
title_full_unstemmed Fungitoxicity against Botrytis cinerea of a Flavonoid Isolated from Pseudognaphalium robustum
title_short Fungitoxicity against Botrytis cinerea of a Flavonoid Isolated from Pseudognaphalium robustum
title_sort fungitoxicity against botrytis cinerea of a flavonoid isolated from pseudognaphalium robustum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6263258/
http://dx.doi.org/10.3390/molecules16053885
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