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Detection of Alpha-Fetoprotein in Hepatocellular Carcinoma Patient Plasma with Graphene Field-Effect Transistor
The detection of alpha-fetoprotein (AFP) in plasma is important in the diagnosis of hepatocellular carcinoma (HCC) in humans. We developed a biosensor to detect AFP in HCC patient plasma and in a phosphate buffer saline (PBS) solution using a graphene field-effect transistor (G-FET). The G-FET was f...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6263997/ https://www.ncbi.nlm.nih.gov/pubmed/30463232 http://dx.doi.org/10.3390/s18114032 |
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author | Kim, Dae Hoon Oh, Hong Gi Park, Woo Hwan Jeon, Dong Cheol Lim, Ki Moo Kim, Hyung Jin Jang, Byoung Kuk Song, Kwang Soup |
author_facet | Kim, Dae Hoon Oh, Hong Gi Park, Woo Hwan Jeon, Dong Cheol Lim, Ki Moo Kim, Hyung Jin Jang, Byoung Kuk Song, Kwang Soup |
author_sort | Kim, Dae Hoon |
collection | PubMed |
description | The detection of alpha-fetoprotein (AFP) in plasma is important in the diagnosis of hepatocellular carcinoma (HCC) in humans. We developed a biosensor to detect AFP in HCC patient plasma and in a phosphate buffer saline (PBS) solution using a graphene field-effect transistor (G-FET). The G-FET was functionalized with 1-pyrenebutyric acid N-hydroxysuccinimide ester (PBASE) for immobilization of an anti-AFP antibody. AFP was detected by assessing the shift in the voltage of the Dirac point (ΔV(Dirac)) after binding of AFP to the anti-AFP-immobilized G-FET channel surface. This anti-AFP-immobilized G-FET biosensor was able to detect AFP at a concentration of 0.1 ng mL(−1) in PBS, and the detection sensitivity was 16.91 mV. In HCC patient plasma, the biosensor was able to detect AFP at a concentration of 12.9 ng mL(−1), with a detection sensitivity of 5.68 mV. The sensitivity (ΔV(Dirac)) depended on the concentration of AFP in either PBS or HCC patient plasma. These data suggest that G-FET biosensors could have practical applications in diagnostics. |
format | Online Article Text |
id | pubmed-6263997 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62639972018-12-12 Detection of Alpha-Fetoprotein in Hepatocellular Carcinoma Patient Plasma with Graphene Field-Effect Transistor Kim, Dae Hoon Oh, Hong Gi Park, Woo Hwan Jeon, Dong Cheol Lim, Ki Moo Kim, Hyung Jin Jang, Byoung Kuk Song, Kwang Soup Sensors (Basel) Article The detection of alpha-fetoprotein (AFP) in plasma is important in the diagnosis of hepatocellular carcinoma (HCC) in humans. We developed a biosensor to detect AFP in HCC patient plasma and in a phosphate buffer saline (PBS) solution using a graphene field-effect transistor (G-FET). The G-FET was functionalized with 1-pyrenebutyric acid N-hydroxysuccinimide ester (PBASE) for immobilization of an anti-AFP antibody. AFP was detected by assessing the shift in the voltage of the Dirac point (ΔV(Dirac)) after binding of AFP to the anti-AFP-immobilized G-FET channel surface. This anti-AFP-immobilized G-FET biosensor was able to detect AFP at a concentration of 0.1 ng mL(−1) in PBS, and the detection sensitivity was 16.91 mV. In HCC patient plasma, the biosensor was able to detect AFP at a concentration of 12.9 ng mL(−1), with a detection sensitivity of 5.68 mV. The sensitivity (ΔV(Dirac)) depended on the concentration of AFP in either PBS or HCC patient plasma. These data suggest that G-FET biosensors could have practical applications in diagnostics. MDPI 2018-11-19 /pmc/articles/PMC6263997/ /pubmed/30463232 http://dx.doi.org/10.3390/s18114032 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kim, Dae Hoon Oh, Hong Gi Park, Woo Hwan Jeon, Dong Cheol Lim, Ki Moo Kim, Hyung Jin Jang, Byoung Kuk Song, Kwang Soup Detection of Alpha-Fetoprotein in Hepatocellular Carcinoma Patient Plasma with Graphene Field-Effect Transistor |
title | Detection of Alpha-Fetoprotein in Hepatocellular Carcinoma Patient Plasma with Graphene Field-Effect Transistor |
title_full | Detection of Alpha-Fetoprotein in Hepatocellular Carcinoma Patient Plasma with Graphene Field-Effect Transistor |
title_fullStr | Detection of Alpha-Fetoprotein in Hepatocellular Carcinoma Patient Plasma with Graphene Field-Effect Transistor |
title_full_unstemmed | Detection of Alpha-Fetoprotein in Hepatocellular Carcinoma Patient Plasma with Graphene Field-Effect Transistor |
title_short | Detection of Alpha-Fetoprotein in Hepatocellular Carcinoma Patient Plasma with Graphene Field-Effect Transistor |
title_sort | detection of alpha-fetoprotein in hepatocellular carcinoma patient plasma with graphene field-effect transistor |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6263997/ https://www.ncbi.nlm.nih.gov/pubmed/30463232 http://dx.doi.org/10.3390/s18114032 |
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