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Soil humic acids degrade CWD prions and reduce infectivity
Chronic wasting disease (CWD), an environmentally transmissible, fatal prion disease is endemic in North America, present in South Korea and has recently been confirmed in northern Europe. The expanding geographic range of this contagious disease of free-ranging deer, moose, elk and reindeer has res...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6264147/ https://www.ncbi.nlm.nih.gov/pubmed/30496301 http://dx.doi.org/10.1371/journal.ppat.1007414 |
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author | Kuznetsova, Alsu Cullingham, Catherine McKenzie, Debbie Aiken, Judd M. |
author_facet | Kuznetsova, Alsu Cullingham, Catherine McKenzie, Debbie Aiken, Judd M. |
author_sort | Kuznetsova, Alsu |
collection | PubMed |
description | Chronic wasting disease (CWD), an environmentally transmissible, fatal prion disease is endemic in North America, present in South Korea and has recently been confirmed in northern Europe. The expanding geographic range of this contagious disease of free-ranging deer, moose, elk and reindeer has resulted in increasing levels of prion infectivity in the environment. Soils are involved in CWD horizontal transmission, acting as an environmental reservoir, and soil mineral and organic compounds have the ability to bind prions. Upper horizons of soils are usually enriched with soil organic matter (SOM), however, the role of SOM in prion conservation and mobility remains unclear. In this study, we show that incubation of PrP(CWD) with humic acids (HA), a major SOM compound, affects both the molecular weight and recovery of PrP(CWD). Detection of PrP(CWD) is reduced as HA concentration increases. Native HA extracted from pristine soils also reduces or entirely eliminates PrP(CWD) signal. Incubation of CWD prions with HA significantly increased incubation periods in tgElk mice demonstrating that HA can reduce CWD infectivity. |
format | Online Article Text |
id | pubmed-6264147 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-62641472018-12-19 Soil humic acids degrade CWD prions and reduce infectivity Kuznetsova, Alsu Cullingham, Catherine McKenzie, Debbie Aiken, Judd M. PLoS Pathog Research Article Chronic wasting disease (CWD), an environmentally transmissible, fatal prion disease is endemic in North America, present in South Korea and has recently been confirmed in northern Europe. The expanding geographic range of this contagious disease of free-ranging deer, moose, elk and reindeer has resulted in increasing levels of prion infectivity in the environment. Soils are involved in CWD horizontal transmission, acting as an environmental reservoir, and soil mineral and organic compounds have the ability to bind prions. Upper horizons of soils are usually enriched with soil organic matter (SOM), however, the role of SOM in prion conservation and mobility remains unclear. In this study, we show that incubation of PrP(CWD) with humic acids (HA), a major SOM compound, affects both the molecular weight and recovery of PrP(CWD). Detection of PrP(CWD) is reduced as HA concentration increases. Native HA extracted from pristine soils also reduces or entirely eliminates PrP(CWD) signal. Incubation of CWD prions with HA significantly increased incubation periods in tgElk mice demonstrating that HA can reduce CWD infectivity. Public Library of Science 2018-11-29 /pmc/articles/PMC6264147/ /pubmed/30496301 http://dx.doi.org/10.1371/journal.ppat.1007414 Text en © 2018 Kuznetsova et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Kuznetsova, Alsu Cullingham, Catherine McKenzie, Debbie Aiken, Judd M. Soil humic acids degrade CWD prions and reduce infectivity |
title | Soil humic acids degrade CWD prions and reduce infectivity |
title_full | Soil humic acids degrade CWD prions and reduce infectivity |
title_fullStr | Soil humic acids degrade CWD prions and reduce infectivity |
title_full_unstemmed | Soil humic acids degrade CWD prions and reduce infectivity |
title_short | Soil humic acids degrade CWD prions and reduce infectivity |
title_sort | soil humic acids degrade cwd prions and reduce infectivity |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6264147/ https://www.ncbi.nlm.nih.gov/pubmed/30496301 http://dx.doi.org/10.1371/journal.ppat.1007414 |
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