Cargando…

Soil humic acids degrade CWD prions and reduce infectivity

Chronic wasting disease (CWD), an environmentally transmissible, fatal prion disease is endemic in North America, present in South Korea and has recently been confirmed in northern Europe. The expanding geographic range of this contagious disease of free-ranging deer, moose, elk and reindeer has res...

Descripción completa

Detalles Bibliográficos
Autores principales: Kuznetsova, Alsu, Cullingham, Catherine, McKenzie, Debbie, Aiken, Judd M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6264147/
https://www.ncbi.nlm.nih.gov/pubmed/30496301
http://dx.doi.org/10.1371/journal.ppat.1007414
_version_ 1783375429661360128
author Kuznetsova, Alsu
Cullingham, Catherine
McKenzie, Debbie
Aiken, Judd M.
author_facet Kuznetsova, Alsu
Cullingham, Catherine
McKenzie, Debbie
Aiken, Judd M.
author_sort Kuznetsova, Alsu
collection PubMed
description Chronic wasting disease (CWD), an environmentally transmissible, fatal prion disease is endemic in North America, present in South Korea and has recently been confirmed in northern Europe. The expanding geographic range of this contagious disease of free-ranging deer, moose, elk and reindeer has resulted in increasing levels of prion infectivity in the environment. Soils are involved in CWD horizontal transmission, acting as an environmental reservoir, and soil mineral and organic compounds have the ability to bind prions. Upper horizons of soils are usually enriched with soil organic matter (SOM), however, the role of SOM in prion conservation and mobility remains unclear. In this study, we show that incubation of PrP(CWD) with humic acids (HA), a major SOM compound, affects both the molecular weight and recovery of PrP(CWD). Detection of PrP(CWD) is reduced as HA concentration increases. Native HA extracted from pristine soils also reduces or entirely eliminates PrP(CWD) signal. Incubation of CWD prions with HA significantly increased incubation periods in tgElk mice demonstrating that HA can reduce CWD infectivity.
format Online
Article
Text
id pubmed-6264147
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-62641472018-12-19 Soil humic acids degrade CWD prions and reduce infectivity Kuznetsova, Alsu Cullingham, Catherine McKenzie, Debbie Aiken, Judd M. PLoS Pathog Research Article Chronic wasting disease (CWD), an environmentally transmissible, fatal prion disease is endemic in North America, present in South Korea and has recently been confirmed in northern Europe. The expanding geographic range of this contagious disease of free-ranging deer, moose, elk and reindeer has resulted in increasing levels of prion infectivity in the environment. Soils are involved in CWD horizontal transmission, acting as an environmental reservoir, and soil mineral and organic compounds have the ability to bind prions. Upper horizons of soils are usually enriched with soil organic matter (SOM), however, the role of SOM in prion conservation and mobility remains unclear. In this study, we show that incubation of PrP(CWD) with humic acids (HA), a major SOM compound, affects both the molecular weight and recovery of PrP(CWD). Detection of PrP(CWD) is reduced as HA concentration increases. Native HA extracted from pristine soils also reduces or entirely eliminates PrP(CWD) signal. Incubation of CWD prions with HA significantly increased incubation periods in tgElk mice demonstrating that HA can reduce CWD infectivity. Public Library of Science 2018-11-29 /pmc/articles/PMC6264147/ /pubmed/30496301 http://dx.doi.org/10.1371/journal.ppat.1007414 Text en © 2018 Kuznetsova et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Kuznetsova, Alsu
Cullingham, Catherine
McKenzie, Debbie
Aiken, Judd M.
Soil humic acids degrade CWD prions and reduce infectivity
title Soil humic acids degrade CWD prions and reduce infectivity
title_full Soil humic acids degrade CWD prions and reduce infectivity
title_fullStr Soil humic acids degrade CWD prions and reduce infectivity
title_full_unstemmed Soil humic acids degrade CWD prions and reduce infectivity
title_short Soil humic acids degrade CWD prions and reduce infectivity
title_sort soil humic acids degrade cwd prions and reduce infectivity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6264147/
https://www.ncbi.nlm.nih.gov/pubmed/30496301
http://dx.doi.org/10.1371/journal.ppat.1007414
work_keys_str_mv AT kuznetsovaalsu soilhumicacidsdegradecwdprionsandreduceinfectivity
AT cullinghamcatherine soilhumicacidsdegradecwdprionsandreduceinfectivity
AT mckenziedebbie soilhumicacidsdegradecwdprionsandreduceinfectivity
AT aikenjuddm soilhumicacidsdegradecwdprionsandreduceinfectivity