Physicochemical Characteristics and Immunomodulatory Activities of Three Polysaccharide-Protein Complexes of Longan Pulp

Three polysaccharide-protein complexes of longan pulp (LP1-3) were isolated in this work. Their physicochemical characteristics and immunomodulatory effects on splenocytes, natural killer (NK) cells and macrophages in vitro were investigated. The carbohydrate portions of LP1-3 were principally compo...

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Autores principales: Yi, Yang, Liao, Sen Tai, Zhang, Ming Wei, Shi, John, Zhang, Rui Fen, Deng, Yuan Yuan, Wei, Zhen Cheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6264155/
https://www.ncbi.nlm.nih.gov/pubmed/21778950
http://dx.doi.org/10.3390/molecules16076148
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author Yi, Yang
Liao, Sen Tai
Zhang, Ming Wei
Shi, John
Zhang, Rui Fen
Deng, Yuan Yuan
Wei, Zhen Cheng
author_facet Yi, Yang
Liao, Sen Tai
Zhang, Ming Wei
Shi, John
Zhang, Rui Fen
Deng, Yuan Yuan
Wei, Zhen Cheng
author_sort Yi, Yang
collection PubMed
description Three polysaccharide-protein complexes of longan pulp (LP1-3) were isolated in this work. Their physicochemical characteristics and immunomodulatory effects on splenocytes, natural killer (NK) cells and macrophages in vitro were investigated. The carbohydrate portions of LP1-3 were principally composed of glucose, arabinose and mannose. LP3 displayed the maximal moisture absorption, and the thermal stability of LP2 was obviously higher than that of LP1 and LP3. All of them showed the characteristic polysaccharide and protein bands in the Fourier Transform Infrared (FTIR) spectrum. For a certain dose, all the fractions could significantly stimulate splenocyte proliferation, macrophage phagocytosis against neutral red, and NK cell cytotoxicity against YAC-1 lymphoma cell (P < 0.05). The results demonstrated that the polysaccharide-protein complexes of longan pulp have medical potential as immunotherapeutic adjuvants due to their immunomodulatory activities.
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spelling pubmed-62641552018-12-10 Physicochemical Characteristics and Immunomodulatory Activities of Three Polysaccharide-Protein Complexes of Longan Pulp Yi, Yang Liao, Sen Tai Zhang, Ming Wei Shi, John Zhang, Rui Fen Deng, Yuan Yuan Wei, Zhen Cheng Molecules Article Three polysaccharide-protein complexes of longan pulp (LP1-3) were isolated in this work. Their physicochemical characteristics and immunomodulatory effects on splenocytes, natural killer (NK) cells and macrophages in vitro were investigated. The carbohydrate portions of LP1-3 were principally composed of glucose, arabinose and mannose. LP3 displayed the maximal moisture absorption, and the thermal stability of LP2 was obviously higher than that of LP1 and LP3. All of them showed the characteristic polysaccharide and protein bands in the Fourier Transform Infrared (FTIR) spectrum. For a certain dose, all the fractions could significantly stimulate splenocyte proliferation, macrophage phagocytosis against neutral red, and NK cell cytotoxicity against YAC-1 lymphoma cell (P < 0.05). The results demonstrated that the polysaccharide-protein complexes of longan pulp have medical potential as immunotherapeutic adjuvants due to their immunomodulatory activities. MDPI 2011-07-21 /pmc/articles/PMC6264155/ /pubmed/21778950 http://dx.doi.org/10.3390/molecules16076148 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Yi, Yang
Liao, Sen Tai
Zhang, Ming Wei
Shi, John
Zhang, Rui Fen
Deng, Yuan Yuan
Wei, Zhen Cheng
Physicochemical Characteristics and Immunomodulatory Activities of Three Polysaccharide-Protein Complexes of Longan Pulp
title Physicochemical Characteristics and Immunomodulatory Activities of Three Polysaccharide-Protein Complexes of Longan Pulp
title_full Physicochemical Characteristics and Immunomodulatory Activities of Three Polysaccharide-Protein Complexes of Longan Pulp
title_fullStr Physicochemical Characteristics and Immunomodulatory Activities of Three Polysaccharide-Protein Complexes of Longan Pulp
title_full_unstemmed Physicochemical Characteristics and Immunomodulatory Activities of Three Polysaccharide-Protein Complexes of Longan Pulp
title_short Physicochemical Characteristics and Immunomodulatory Activities of Three Polysaccharide-Protein Complexes of Longan Pulp
title_sort physicochemical characteristics and immunomodulatory activities of three polysaccharide-protein complexes of longan pulp
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6264155/
https://www.ncbi.nlm.nih.gov/pubmed/21778950
http://dx.doi.org/10.3390/molecules16076148
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