Cargando…
Transcriptional and Functional Analysis Shows Sodium Houttuyfonate-Mediated Inhibition of Autolysis in Staphylococcus aureus
Sodium houttuyfonate (SH), an addition compound of sodium bisulfite and houttuynin,showed in vitro antibacterial activity against 21 Staphylococcus aureus (S. aureus) strains grown in planktonic cultures. Microarray results showed decreased levels of autolysin atl, sle1, cidA and lytN transcripts in...
Autores principales: | , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6264171/ https://www.ncbi.nlm.nih.gov/pubmed/22019573 http://dx.doi.org/10.3390/molecules16108848 |
_version_ | 1783375435252367360 |
---|---|
author | Liu, Guoxing Xiang, Hua Tang, Xudong Zhang, Kaiyu Wu, Xiuping Wang, Xuelin Guo, Na Feng, Haihua Wang, Guangming Liu, Lihui Shi, Qiyun Shen, Fengge Xing, Mingxun Yuan, Peng Liu, Mingyuan Yu, Lu |
author_facet | Liu, Guoxing Xiang, Hua Tang, Xudong Zhang, Kaiyu Wu, Xiuping Wang, Xuelin Guo, Na Feng, Haihua Wang, Guangming Liu, Lihui Shi, Qiyun Shen, Fengge Xing, Mingxun Yuan, Peng Liu, Mingyuan Yu, Lu |
author_sort | Liu, Guoxing |
collection | PubMed |
description | Sodium houttuyfonate (SH), an addition compound of sodium bisulfite and houttuynin,showed in vitro antibacterial activity against 21 Staphylococcus aureus (S. aureus) strains grown in planktonic cultures. Microarray results showed decreased levels of autolysin atl, sle1, cidA and lytN transcripts in the SH-treated strain as compared to the control strain, consistent with the induction of the autolytic repressors lrgAB and sarA and with the downregulation of the positive regulators agrA and RNAIII. Triton X-100-induced autolysis was significantly decreased by SH in S. aureus ATCC 25923, and quantitative bacteriolytic assays and zymographic analysis demonstrated SH-mediated reduction of extracellular murein hydrolase activity in these cells. Anti-biofilm assay showed that SH is poorly active against S. aureus grown in biofilm cultures, whereas SH diminished the amounts of extracellular DNA (eDNA) of S. aureus in a dose-dependent manner, which suggested that SH may impede biofilm formation by reducing the expression of cidA to inhibit autolysis and eDNA release in the early phase. Some of the microarray results were confirmed by real-time RT-PCR. |
format | Online Article Text |
id | pubmed-6264171 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62641712018-12-10 Transcriptional and Functional Analysis Shows Sodium Houttuyfonate-Mediated Inhibition of Autolysis in Staphylococcus aureus Liu, Guoxing Xiang, Hua Tang, Xudong Zhang, Kaiyu Wu, Xiuping Wang, Xuelin Guo, Na Feng, Haihua Wang, Guangming Liu, Lihui Shi, Qiyun Shen, Fengge Xing, Mingxun Yuan, Peng Liu, Mingyuan Yu, Lu Molecules Article Sodium houttuyfonate (SH), an addition compound of sodium bisulfite and houttuynin,showed in vitro antibacterial activity against 21 Staphylococcus aureus (S. aureus) strains grown in planktonic cultures. Microarray results showed decreased levels of autolysin atl, sle1, cidA and lytN transcripts in the SH-treated strain as compared to the control strain, consistent with the induction of the autolytic repressors lrgAB and sarA and with the downregulation of the positive regulators agrA and RNAIII. Triton X-100-induced autolysis was significantly decreased by SH in S. aureus ATCC 25923, and quantitative bacteriolytic assays and zymographic analysis demonstrated SH-mediated reduction of extracellular murein hydrolase activity in these cells. Anti-biofilm assay showed that SH is poorly active against S. aureus grown in biofilm cultures, whereas SH diminished the amounts of extracellular DNA (eDNA) of S. aureus in a dose-dependent manner, which suggested that SH may impede biofilm formation by reducing the expression of cidA to inhibit autolysis and eDNA release in the early phase. Some of the microarray results were confirmed by real-time RT-PCR. MDPI 2011-10-21 /pmc/articles/PMC6264171/ /pubmed/22019573 http://dx.doi.org/10.3390/molecules16108848 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Liu, Guoxing Xiang, Hua Tang, Xudong Zhang, Kaiyu Wu, Xiuping Wang, Xuelin Guo, Na Feng, Haihua Wang, Guangming Liu, Lihui Shi, Qiyun Shen, Fengge Xing, Mingxun Yuan, Peng Liu, Mingyuan Yu, Lu Transcriptional and Functional Analysis Shows Sodium Houttuyfonate-Mediated Inhibition of Autolysis in Staphylococcus aureus |
title | Transcriptional and Functional Analysis Shows Sodium Houttuyfonate-Mediated Inhibition of Autolysis in Staphylococcus aureus |
title_full | Transcriptional and Functional Analysis Shows Sodium Houttuyfonate-Mediated Inhibition of Autolysis in Staphylococcus aureus |
title_fullStr | Transcriptional and Functional Analysis Shows Sodium Houttuyfonate-Mediated Inhibition of Autolysis in Staphylococcus aureus |
title_full_unstemmed | Transcriptional and Functional Analysis Shows Sodium Houttuyfonate-Mediated Inhibition of Autolysis in Staphylococcus aureus |
title_short | Transcriptional and Functional Analysis Shows Sodium Houttuyfonate-Mediated Inhibition of Autolysis in Staphylococcus aureus |
title_sort | transcriptional and functional analysis shows sodium houttuyfonate-mediated inhibition of autolysis in staphylococcus aureus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6264171/ https://www.ncbi.nlm.nih.gov/pubmed/22019573 http://dx.doi.org/10.3390/molecules16108848 |
work_keys_str_mv | AT liuguoxing transcriptionalandfunctionalanalysisshowssodiumhouttuyfonatemediatedinhibitionofautolysisinstaphylococcusaureus AT xianghua transcriptionalandfunctionalanalysisshowssodiumhouttuyfonatemediatedinhibitionofautolysisinstaphylococcusaureus AT tangxudong transcriptionalandfunctionalanalysisshowssodiumhouttuyfonatemediatedinhibitionofautolysisinstaphylococcusaureus AT zhangkaiyu transcriptionalandfunctionalanalysisshowssodiumhouttuyfonatemediatedinhibitionofautolysisinstaphylococcusaureus AT wuxiuping transcriptionalandfunctionalanalysisshowssodiumhouttuyfonatemediatedinhibitionofautolysisinstaphylococcusaureus AT wangxuelin transcriptionalandfunctionalanalysisshowssodiumhouttuyfonatemediatedinhibitionofautolysisinstaphylococcusaureus AT guona transcriptionalandfunctionalanalysisshowssodiumhouttuyfonatemediatedinhibitionofautolysisinstaphylococcusaureus AT fenghaihua transcriptionalandfunctionalanalysisshowssodiumhouttuyfonatemediatedinhibitionofautolysisinstaphylococcusaureus AT wangguangming transcriptionalandfunctionalanalysisshowssodiumhouttuyfonatemediatedinhibitionofautolysisinstaphylococcusaureus AT liulihui transcriptionalandfunctionalanalysisshowssodiumhouttuyfonatemediatedinhibitionofautolysisinstaphylococcusaureus AT shiqiyun transcriptionalandfunctionalanalysisshowssodiumhouttuyfonatemediatedinhibitionofautolysisinstaphylococcusaureus AT shenfengge transcriptionalandfunctionalanalysisshowssodiumhouttuyfonatemediatedinhibitionofautolysisinstaphylococcusaureus AT xingmingxun transcriptionalandfunctionalanalysisshowssodiumhouttuyfonatemediatedinhibitionofautolysisinstaphylococcusaureus AT yuanpeng transcriptionalandfunctionalanalysisshowssodiumhouttuyfonatemediatedinhibitionofautolysisinstaphylococcusaureus AT liumingyuan transcriptionalandfunctionalanalysisshowssodiumhouttuyfonatemediatedinhibitionofautolysisinstaphylococcusaureus AT yulu transcriptionalandfunctionalanalysisshowssodiumhouttuyfonatemediatedinhibitionofautolysisinstaphylococcusaureus |