Isolation and Purification of Oridonin from the Whole Plant of Isodon rubescens by High-Speed Counter-Current Chromatography

Semi-preparative high-speed counter-current chromatography (HSCCC) was successfully used for isolation and purification of oridonin from Isodon rubescens by using a two-phase-solvent system composed of n-hexane-ethyl acetate-methanol-water (2.8:5:2.8:5, v/v/v/v). The targeted compound isolated, coll...

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Detalles Bibliográficos
Autores principales: He, Fa, Bai, Yuhua, Wang, Jing, Wei, Jing, Yu, ChunYue, Li, Sen, Yang, Weili, Han, Chenghua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6264197/
https://www.ncbi.nlm.nih.gov/pubmed/22143552
http://dx.doi.org/10.3390/molecules16097949
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author He, Fa
Bai, Yuhua
Wang, Jing
Wei, Jing
Yu, ChunYue
Li, Sen
Yang, Weili
Han, Chenghua
author_facet He, Fa
Bai, Yuhua
Wang, Jing
Wei, Jing
Yu, ChunYue
Li, Sen
Yang, Weili
Han, Chenghua
author_sort He, Fa
collection PubMed
description Semi-preparative high-speed counter-current chromatography (HSCCC) was successfully used for isolation and purification of oridonin from Isodon rubescens by using a two-phase-solvent system composed of n-hexane-ethyl acetate-methanol-water (2.8:5:2.8:5, v/v/v/v). The targeted compound isolated, collected and purified by HSCCC was analyzed by high performance liquid chromatography (HPLC). A total of 40.6 mg of oridonin with the purity of 73.5% was obtained in less than 100 min from 100 mg of crude Isodon rubescens extract. The chemical structure of the compound was identified by IR, (1)H-NMR and (13)C-NMR.
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spelling pubmed-62641972018-12-10 Isolation and Purification of Oridonin from the Whole Plant of Isodon rubescens by High-Speed Counter-Current Chromatography He, Fa Bai, Yuhua Wang, Jing Wei, Jing Yu, ChunYue Li, Sen Yang, Weili Han, Chenghua Molecules Article Semi-preparative high-speed counter-current chromatography (HSCCC) was successfully used for isolation and purification of oridonin from Isodon rubescens by using a two-phase-solvent system composed of n-hexane-ethyl acetate-methanol-water (2.8:5:2.8:5, v/v/v/v). The targeted compound isolated, collected and purified by HSCCC was analyzed by high performance liquid chromatography (HPLC). A total of 40.6 mg of oridonin with the purity of 73.5% was obtained in less than 100 min from 100 mg of crude Isodon rubescens extract. The chemical structure of the compound was identified by IR, (1)H-NMR and (13)C-NMR. MDPI 2011-09-14 /pmc/articles/PMC6264197/ /pubmed/22143552 http://dx.doi.org/10.3390/molecules16097949 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
He, Fa
Bai, Yuhua
Wang, Jing
Wei, Jing
Yu, ChunYue
Li, Sen
Yang, Weili
Han, Chenghua
Isolation and Purification of Oridonin from the Whole Plant of Isodon rubescens by High-Speed Counter-Current Chromatography
title Isolation and Purification of Oridonin from the Whole Plant of Isodon rubescens by High-Speed Counter-Current Chromatography
title_full Isolation and Purification of Oridonin from the Whole Plant of Isodon rubescens by High-Speed Counter-Current Chromatography
title_fullStr Isolation and Purification of Oridonin from the Whole Plant of Isodon rubescens by High-Speed Counter-Current Chromatography
title_full_unstemmed Isolation and Purification of Oridonin from the Whole Plant of Isodon rubescens by High-Speed Counter-Current Chromatography
title_short Isolation and Purification of Oridonin from the Whole Plant of Isodon rubescens by High-Speed Counter-Current Chromatography
title_sort isolation and purification of oridonin from the whole plant of isodon rubescens by high-speed counter-current chromatography
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6264197/
https://www.ncbi.nlm.nih.gov/pubmed/22143552
http://dx.doi.org/10.3390/molecules16097949
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