Cleavage of Oligonucleotides Containing a P3’→N5’ Phosphoramidate Linkage Mediated by Single-Stranded Oligonucleotide Templates

Double-stranded DNA (dsDNA) templates can hybridize to and accelerate cleavage of oligonucleotides containing a P3’→N5’ phosphoramidate (P-N) linkage. This dsDNA-templated cleavage of P-N linkages could be due to conformational strain placed on the linkage upon triplex formation. To determine whethe...

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Autores principales: Ito, Kosuke Ramon, Kodama, Tetsuya, Makimura, Futaba, Hosoki, Noritsugu, Osaki, Tomohisa, Orita, Ayako, Imanishi, Takeshi, Obika, Satoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2011
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6264227/
https://www.ncbi.nlm.nih.gov/pubmed/22186956
http://dx.doi.org/10.3390/molecules161210695
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author Ito, Kosuke Ramon
Kodama, Tetsuya
Makimura, Futaba
Hosoki, Noritsugu
Osaki, Tomohisa
Orita, Ayako
Imanishi, Takeshi
Obika, Satoshi
author_facet Ito, Kosuke Ramon
Kodama, Tetsuya
Makimura, Futaba
Hosoki, Noritsugu
Osaki, Tomohisa
Orita, Ayako
Imanishi, Takeshi
Obika, Satoshi
author_sort Ito, Kosuke Ramon
collection PubMed
description Double-stranded DNA (dsDNA) templates can hybridize to and accelerate cleavage of oligonucleotides containing a P3’→N5’ phosphoramidate (P-N) linkage. This dsDNA-templated cleavage of P-N linkages could be due to conformational strain placed on the linkage upon triplex formation. To determine whether duplex formation also induced conformational strain, we examined the reactivity of the oligonucleotides with a P-N linkage in the presence of single-stranded templates, and compared these reactions to those with dsDNA templates. P-N oligonucleotides that are cleaved upon duplex formation could be used as probes to detect single-stranded nucleic acids.
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spelling pubmed-62642272018-12-10 Cleavage of Oligonucleotides Containing a P3’→N5’ Phosphoramidate Linkage Mediated by Single-Stranded Oligonucleotide Templates Ito, Kosuke Ramon Kodama, Tetsuya Makimura, Futaba Hosoki, Noritsugu Osaki, Tomohisa Orita, Ayako Imanishi, Takeshi Obika, Satoshi Molecules Article Double-stranded DNA (dsDNA) templates can hybridize to and accelerate cleavage of oligonucleotides containing a P3’→N5’ phosphoramidate (P-N) linkage. This dsDNA-templated cleavage of P-N linkages could be due to conformational strain placed on the linkage upon triplex formation. To determine whether duplex formation also induced conformational strain, we examined the reactivity of the oligonucleotides with a P-N linkage in the presence of single-stranded templates, and compared these reactions to those with dsDNA templates. P-N oligonucleotides that are cleaved upon duplex formation could be used as probes to detect single-stranded nucleic acids. MDPI 2011-12-20 /pmc/articles/PMC6264227/ /pubmed/22186956 http://dx.doi.org/10.3390/molecules161210695 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Ito, Kosuke Ramon
Kodama, Tetsuya
Makimura, Futaba
Hosoki, Noritsugu
Osaki, Tomohisa
Orita, Ayako
Imanishi, Takeshi
Obika, Satoshi
Cleavage of Oligonucleotides Containing a P3’→N5’ Phosphoramidate Linkage Mediated by Single-Stranded Oligonucleotide Templates
title Cleavage of Oligonucleotides Containing a P3’→N5’ Phosphoramidate Linkage Mediated by Single-Stranded Oligonucleotide Templates
title_full Cleavage of Oligonucleotides Containing a P3’→N5’ Phosphoramidate Linkage Mediated by Single-Stranded Oligonucleotide Templates
title_fullStr Cleavage of Oligonucleotides Containing a P3’→N5’ Phosphoramidate Linkage Mediated by Single-Stranded Oligonucleotide Templates
title_full_unstemmed Cleavage of Oligonucleotides Containing a P3’→N5’ Phosphoramidate Linkage Mediated by Single-Stranded Oligonucleotide Templates
title_short Cleavage of Oligonucleotides Containing a P3’→N5’ Phosphoramidate Linkage Mediated by Single-Stranded Oligonucleotide Templates
title_sort cleavage of oligonucleotides containing a p3’→n5’ phosphoramidate linkage mediated by single-stranded oligonucleotide templates
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6264227/
https://www.ncbi.nlm.nih.gov/pubmed/22186956
http://dx.doi.org/10.3390/molecules161210695
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