Cargando…

HPLC and Anti-Inflammatory Studies of the Flavonoid Rich Chloroform Extract Fractionof Orthosiphon Stamineus Leaves

The aim of the present study was to verify the anti-inflammatory activity of Orthosiphon stamineus leaf extracts and to identify the active compound(s) contributing to its anti-inflammatory activity using a developed HPLC method. Active chloroform extract of O. stamineus was fractionated into three...

Descripción completa

Detalles Bibliográficos
Autores principales: Yam, Mun Fei, Lim, Vuanghao, Salman, Ibrahim Muhammad, Ameer, Omar Ziad, Ang, Lee Fung, Rosidah, Noersal, Abdulkarim, Muthanna Fawzy, Abdullah, Ghassan Zuhair, Basir, Rusliza, Sadikun, Amirin, Asmawi, Mohd Zaini
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6264410/
https://www.ncbi.nlm.nih.gov/pubmed/20657453
http://dx.doi.org/10.3390/molecules15064452
_version_ 1783375490107572224
author Yam, Mun Fei
Lim, Vuanghao
Salman, Ibrahim Muhammad
Ameer, Omar Ziad
Ang, Lee Fung
Rosidah, Noersal
Abdulkarim, Muthanna Fawzy
Abdullah, Ghassan Zuhair
Basir, Rusliza
Sadikun, Amirin
Asmawi, Mohd Zaini
author_facet Yam, Mun Fei
Lim, Vuanghao
Salman, Ibrahim Muhammad
Ameer, Omar Ziad
Ang, Lee Fung
Rosidah, Noersal
Abdulkarim, Muthanna Fawzy
Abdullah, Ghassan Zuhair
Basir, Rusliza
Sadikun, Amirin
Asmawi, Mohd Zaini
author_sort Yam, Mun Fei
collection PubMed
description The aim of the present study was to verify the anti-inflammatory activity of Orthosiphon stamineus leaf extracts and to identify the active compound(s) contributing to its anti-inflammatory activity using a developed HPLC method. Active chloroform extract of O. stamineus was fractionated into three fractions using a dry flash column chromatography method. These three fractions were investigated for anti-peritoneal capillary permeability, in vitro nitric oxide scavenging activity, anti-inflammatory and nitric oxide (NO) inhibition using carrageenan-induced hind paw edema method. The flavonoid rich chloroform extract fraction (CF2) [containing sinensetin (2.86% w/w), eupatorin (5.05% w/w) and 3’-hydroxy-5,6,7,4’-tetramethoxyflavone (1.101% w/w)], significantly reduced rat hind paw edema, NO and decreased dye leakage to peritoneal cavity at p < 0.05. IC(50) of in vitro NO scavenging of CF2 was 0.3 mg/mL. These results suggest that the anti-inflammatory properties of these CF2 may possibly be due to the presence of flavonoid compounds capable of affecting the NO pathway.
format Online
Article
Text
id pubmed-6264410
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-62644102018-12-04 HPLC and Anti-Inflammatory Studies of the Flavonoid Rich Chloroform Extract Fractionof Orthosiphon Stamineus Leaves Yam, Mun Fei Lim, Vuanghao Salman, Ibrahim Muhammad Ameer, Omar Ziad Ang, Lee Fung Rosidah, Noersal Abdulkarim, Muthanna Fawzy Abdullah, Ghassan Zuhair Basir, Rusliza Sadikun, Amirin Asmawi, Mohd Zaini Molecules Article The aim of the present study was to verify the anti-inflammatory activity of Orthosiphon stamineus leaf extracts and to identify the active compound(s) contributing to its anti-inflammatory activity using a developed HPLC method. Active chloroform extract of O. stamineus was fractionated into three fractions using a dry flash column chromatography method. These three fractions were investigated for anti-peritoneal capillary permeability, in vitro nitric oxide scavenging activity, anti-inflammatory and nitric oxide (NO) inhibition using carrageenan-induced hind paw edema method. The flavonoid rich chloroform extract fraction (CF2) [containing sinensetin (2.86% w/w), eupatorin (5.05% w/w) and 3’-hydroxy-5,6,7,4’-tetramethoxyflavone (1.101% w/w)], significantly reduced rat hind paw edema, NO and decreased dye leakage to peritoneal cavity at p < 0.05. IC(50) of in vitro NO scavenging of CF2 was 0.3 mg/mL. These results suggest that the anti-inflammatory properties of these CF2 may possibly be due to the presence of flavonoid compounds capable of affecting the NO pathway. MDPI 2010-06-21 /pmc/articles/PMC6264410/ /pubmed/20657453 http://dx.doi.org/10.3390/molecules15064452 Text en © 2010 by the authors; licensee MDPI, Basel, Switzerland. This article is an Open Access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Yam, Mun Fei
Lim, Vuanghao
Salman, Ibrahim Muhammad
Ameer, Omar Ziad
Ang, Lee Fung
Rosidah, Noersal
Abdulkarim, Muthanna Fawzy
Abdullah, Ghassan Zuhair
Basir, Rusliza
Sadikun, Amirin
Asmawi, Mohd Zaini
HPLC and Anti-Inflammatory Studies of the Flavonoid Rich Chloroform Extract Fractionof Orthosiphon Stamineus Leaves
title HPLC and Anti-Inflammatory Studies of the Flavonoid Rich Chloroform Extract Fractionof Orthosiphon Stamineus Leaves
title_full HPLC and Anti-Inflammatory Studies of the Flavonoid Rich Chloroform Extract Fractionof Orthosiphon Stamineus Leaves
title_fullStr HPLC and Anti-Inflammatory Studies of the Flavonoid Rich Chloroform Extract Fractionof Orthosiphon Stamineus Leaves
title_full_unstemmed HPLC and Anti-Inflammatory Studies of the Flavonoid Rich Chloroform Extract Fractionof Orthosiphon Stamineus Leaves
title_short HPLC and Anti-Inflammatory Studies of the Flavonoid Rich Chloroform Extract Fractionof Orthosiphon Stamineus Leaves
title_sort hplc and anti-inflammatory studies of the flavonoid rich chloroform extract fractionof orthosiphon stamineus leaves
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6264410/
https://www.ncbi.nlm.nih.gov/pubmed/20657453
http://dx.doi.org/10.3390/molecules15064452
work_keys_str_mv AT yammunfei hplcandantiinflammatorystudiesoftheflavonoidrichchloroformextractfractionoforthosiphonstamineusleaves
AT limvuanghao hplcandantiinflammatorystudiesoftheflavonoidrichchloroformextractfractionoforthosiphonstamineusleaves
AT salmanibrahimmuhammad hplcandantiinflammatorystudiesoftheflavonoidrichchloroformextractfractionoforthosiphonstamineusleaves
AT ameeromarziad hplcandantiinflammatorystudiesoftheflavonoidrichchloroformextractfractionoforthosiphonstamineusleaves
AT angleefung hplcandantiinflammatorystudiesoftheflavonoidrichchloroformextractfractionoforthosiphonstamineusleaves
AT rosidahnoersal hplcandantiinflammatorystudiesoftheflavonoidrichchloroformextractfractionoforthosiphonstamineusleaves
AT abdulkarimmuthannafawzy hplcandantiinflammatorystudiesoftheflavonoidrichchloroformextractfractionoforthosiphonstamineusleaves
AT abdullahghassanzuhair hplcandantiinflammatorystudiesoftheflavonoidrichchloroformextractfractionoforthosiphonstamineusleaves
AT basirrusliza hplcandantiinflammatorystudiesoftheflavonoidrichchloroformextractfractionoforthosiphonstamineusleaves
AT sadikunamirin hplcandantiinflammatorystudiesoftheflavonoidrichchloroformextractfractionoforthosiphonstamineusleaves
AT asmawimohdzaini hplcandantiinflammatorystudiesoftheflavonoidrichchloroformextractfractionoforthosiphonstamineusleaves