HPLC Determination of Antilipoxygenase Activity of a Water Infusion of Ligustrum vulgare L. Leaves and Some of Its Constituents

The aim of the study was a HPLC evaluation of the lipoxygenase activity inhibiting activity of a water infusion of Ligustrum vulgare L. leaves and selected isolates from it. The antiradical activity of the water infusion was determined using DPPH, ABTS and FRAP tests. Oleuropein and echinacoside con...

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Autores principales: Mučaji, Pavel, Záhradníková, Anna, Bezáková, Lýdia, Cupáková, Mária, Rauová, Drahomíra, Nagy, Milan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2011
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6264466/
https://www.ncbi.nlm.nih.gov/pubmed/21959294
http://dx.doi.org/10.3390/molecules16108198
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author Mučaji, Pavel
Záhradníková, Anna
Bezáková, Lýdia
Cupáková, Mária
Rauová, Drahomíra
Nagy, Milan
author_facet Mučaji, Pavel
Záhradníková, Anna
Bezáková, Lýdia
Cupáková, Mária
Rauová, Drahomíra
Nagy, Milan
author_sort Mučaji, Pavel
collection PubMed
description The aim of the study was a HPLC evaluation of the lipoxygenase activity inhibiting activity of a water infusion of Ligustrum vulgare L. leaves and selected isolates from it. The antiradical activity of the water infusion was determined using DPPH, ABTS and FRAP tests. Oleuropein and echinacoside concentrations in the water infusion were determined by HPLC. Water infusion, echinacoside and oleuropein were used for an antilipoxygenase activity assay using lipoxygenase isolated from rat lung cytosol fraction. Activity of 8-LOX, 12-LOX and 15-LOX were monitored through formation of 8-HETE, 12-HETE and 15-HETE, respectively. The water infusion exhibited the highest activity against all lipoxygenases, followed by oleuropein. Echinacoside was ineffective against LOXs in lower concentrations, while higher concentration showed similar inhibition on 8-LOX and 12-LOX. 15-LOX was affected more and the presence of echinacoside remarkably decreased its activity.
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spelling pubmed-62644662018-12-10 HPLC Determination of Antilipoxygenase Activity of a Water Infusion of Ligustrum vulgare L. Leaves and Some of Its Constituents Mučaji, Pavel Záhradníková, Anna Bezáková, Lýdia Cupáková, Mária Rauová, Drahomíra Nagy, Milan Molecules Article The aim of the study was a HPLC evaluation of the lipoxygenase activity inhibiting activity of a water infusion of Ligustrum vulgare L. leaves and selected isolates from it. The antiradical activity of the water infusion was determined using DPPH, ABTS and FRAP tests. Oleuropein and echinacoside concentrations in the water infusion were determined by HPLC. Water infusion, echinacoside and oleuropein were used for an antilipoxygenase activity assay using lipoxygenase isolated from rat lung cytosol fraction. Activity of 8-LOX, 12-LOX and 15-LOX were monitored through formation of 8-HETE, 12-HETE and 15-HETE, respectively. The water infusion exhibited the highest activity against all lipoxygenases, followed by oleuropein. Echinacoside was ineffective against LOXs in lower concentrations, while higher concentration showed similar inhibition on 8-LOX and 12-LOX. 15-LOX was affected more and the presence of echinacoside remarkably decreased its activity. MDPI 2011-09-28 /pmc/articles/PMC6264466/ /pubmed/21959294 http://dx.doi.org/10.3390/molecules16108198 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Mučaji, Pavel
Záhradníková, Anna
Bezáková, Lýdia
Cupáková, Mária
Rauová, Drahomíra
Nagy, Milan
HPLC Determination of Antilipoxygenase Activity of a Water Infusion of Ligustrum vulgare L. Leaves and Some of Its Constituents
title HPLC Determination of Antilipoxygenase Activity of a Water Infusion of Ligustrum vulgare L. Leaves and Some of Its Constituents
title_full HPLC Determination of Antilipoxygenase Activity of a Water Infusion of Ligustrum vulgare L. Leaves and Some of Its Constituents
title_fullStr HPLC Determination of Antilipoxygenase Activity of a Water Infusion of Ligustrum vulgare L. Leaves and Some of Its Constituents
title_full_unstemmed HPLC Determination of Antilipoxygenase Activity of a Water Infusion of Ligustrum vulgare L. Leaves and Some of Its Constituents
title_short HPLC Determination of Antilipoxygenase Activity of a Water Infusion of Ligustrum vulgare L. Leaves and Some of Its Constituents
title_sort hplc determination of antilipoxygenase activity of a water infusion of ligustrum vulgare l. leaves and some of its constituents
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6264466/
https://www.ncbi.nlm.nih.gov/pubmed/21959294
http://dx.doi.org/10.3390/molecules16108198
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