Cargando…

Monitoring the parasite load in chronic Chagas disease patients: comparison between blood culture and quantitative real time PCR

BACKGROUND: Despite the improvements in diagnostic tools for detection of Trypanosoma cruzi in human blood samples, the isolation of parasite from bloodstream in the chronic phase of Chagas disease is challenging. Thus, there is an increasing interest in the development of strategies that allow an a...

Descripción completa

Detalles Bibliográficos
Autores principales:	D’Ávila, Daniella Alchaar, Galvão, Lúcia Maria C., Sousa, Giovane R., Britto, Constança, Moreira, Otacilio C., Chiari, Egler
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2018
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6264508/ https://www.ncbi.nlm.nih.gov/pubmed/30496249 http://dx.doi.org/10.1371/journal.pone.0208133

Ejemplares similares

Usefulness of real time PCR to quantify parasite load in serum samples from chronic Chagas disease patients
por: Melo, Myllena F, et al.
Publicado: (2015)

Exploring the parasite load and molecular diversity of Trypanosoma cruzi in patients with chronic Chagas disease from different regions of Brazil
por: Rodrigues-dos-Santos, Ícaro, et al.
Publicado: (2018)

Memory impairment in chronic experimental Chagas disease: Benznidazole therapy reversed cognitive deficit in association with reduction of parasite load and oxidative stress in the nervous tissue
por: Vilar-Pereira, Glaucia, et al.
Publicado: (2021)

Development of conventional and real-time multiplex PCR-based assays for estimation of natural infection rates and Trypanosoma cruzi load in triatomine vectors
por: Moreira, Otacilio C., et al.
Publicado: (2017)

High Parasitic Loads Quantified in Sylvatic Triatoma melanica, a Chagas Disease Vector
por: Valença-Barbosa, Carolina, et al.
Publicado: (2022)

Tumor Necrosis Factor Is a Therapeutic Target for Immunological Unbalance and Cardiac Abnormalities in Chronic Experimental Chagas' Heart Disease
por: Pereira, Isabela Resende, et al.
Publicado: (2014)

Combination Chemotherapy with Suboptimal Doses of Benznidazole and Pentoxifylline Sustains Partial Reversion of Experimental Chagas' Heart Disease
por: Vilar-Pereira, Glaucia, et al.
Publicado: (2016)

Revisiting Chagas' disease diagnostic strategies in light of different scenarios of Trypanosoma cruzi infection
por: Britto, Constança Carvalho
Publicado: (2022)

Whole genome sequencing of Trypanosoma cruzi field isolates reveals extensive genomic variability and complex aneuploidy patterns within TcII DTU
por: Reis-Cunha, João Luís, et al.
Publicado: (2018)

Validation of the NAT Chagas IVD Kit for the Detection and Quantification of Trypanosoma cruzi in Blood Samples of Patients with Chagas Disease
por: Moreira, Otacilio C., et al.
Publicado: (2023)

Accurate Real-Time PCR Strategy for Monitoring Bloodstream Parasitic Loads in Chagas Disease Patients
por: Duffy, Tomas, et al.
Publicado: (2009)

SYBR Green-based Real-Time PCR targeting kinetoplast DNA can be used to discriminate between the main etiologic agents of Brazilian cutaneous and visceral leishmaniases
por: Pita-Pereira, Daniela, et al.
Publicado: (2012)

HRM Accuracy and Limitations as a Species Typing Tool for Leishmania Parasites
por: Filgueira, Camila Patricio Braga, et al.
Publicado: (2023)

Development of real-time PCR assays for evaluation of immune response and parasite load in golden hamster (Mesocricetus auratus) infected by Leishmania (Viannia) braziliensis
por: Ribeiro-Romão, Raquel Peralva, et al.
Publicado: (2016)

Establishment of a murine model of congenital toxoplasmosis and validation of a qPCR assay to assess the parasite load in maternal and fetal tissues
por: Souza, Jéssica S., et al.
Publicado: (2023)

Unraveling Chagas disease transmission through the oral route: Gateways to Trypanosoma cruzi infection and target tissues
por: Silva-dos-Santos, Danielle, et al.
Publicado: (2017)

Comparison and clinical validation of qPCR assays targeting Leishmania 18S rDNA and HSP70 genes in patients with American Tegumentary Leishmaniasis
por: Filgueira, Camila Patricio Braga, et al.
Publicado: (2020)

Inflammation Enhances the Risks of Stroke and Death in Chronic Chagas Disease Patients
por: Guedes, Paulo Marcos Matta, et al.
Publicado: (2016)

Lipid Bodies as Sites of Prostaglandin E2 Synthesis During Chagas Disease: Impact in the Parasite Escape Mechanism
por: de Almeida, Patrícia E., et al.
Publicado: (2018)

Association between Trypanosoma cruzi DTU TcII and chronic Chagas disease clinical presentation and outcome in an urban cohort in Brazil
por: Nielebock, Marco Antonio Prates, et al.
Publicado: (2020)

New approaches for the standardization and validation of a real-time qPCR assay using TaqMan probes for quantification of yellow fever virus on clinical samples with high quality parameters
por: Fernandes-Monteiro, Alice G, et al.
Publicado: (2015)

Parasite load evaluation by qPCR and blood culture in Chagas disease and HIV co-infected patients under antiretroviral therapy
por: Marcon, Gláucia Elisete Barbosa, et al.
Publicado: (2022)

Biomarkers and Echocardiographic Predictors of Cardiovascular Outcome in Patients With Chronic Chagas Disease
por: Mendes, Veronica G., et al.
Publicado: (2023)

Corrigendum: Lipid Bodies as Sites of Prostaglandin E2 Synthesis During Chagas Disease: Impact in the Parasite Escape Mechanism
por: de Almeida, Patrícia E., et al.
Publicado: (2018)

Cruzipain Promotes Trypanosoma cruzi Adhesion to Rhodnius prolixus Midgut
por: Uehara, Lívia Almeida, et al.
Publicado: (2012)

Sexual transmission of American trypanosomiasis in humans: a new potential pandemic route for Chagas parasites
por: Araujo, Perla F, et al.
Publicado: (2017)

Parasitic Load Correlates With Left Ventricular Dysfunction in Patients With Chronic Chagas Cardiomyopathy
por: de Oliveira, Maykon Tavares, et al.
Publicado: (2021)

NOD2 receptor is crucial for protecting against the digestive form of Chagas disease
por: Pereira, Nathalie de Sena, et al.
Publicado: (2020)

Differential Activation of Human Monocytes and Lymphocytes by Distinct Strains of Trypanosoma cruzi
por: Magalhães, Luísa M. D., et al.
Publicado: (2015)

Chagas Disease Control—Many Approaches to Prospect
por: Branquinha, Marta H., et al.
Publicado: (2023)

Cardiac Abnormalities in a Predictive Mouse Model of Chagas Disease
por: Francisco, Amanda Fortes, et al.
Publicado: (2023)

Assessing the mitochondrial DNA diversity of the Chagas disease vector Triatoma sordida (Hemiptera: Reduviidae)
por: Pessoa, Grasielle Caldas D‘Ávila, et al.
Publicado: (2016)

Neglected Parasitic Infections in the United States: Chagas Disease
por: Montgomery, Susan P., et al.
Publicado: (2014)

Aplastic Anemia and Chagas Disease: T. cruzi Parasitemia Monitoring by Quantitative PCR and Preemptive Antiparasitic Therapy
por: Carvalho, Noêmia Barbosa, et al.
Publicado: (2022)

Trypanosoma cruzi in the Chicken Model: Chagas-Like Heart Disease in the Absence of Parasitism
por: Teixeira, Antonio R. L., et al.
Publicado: (2011)

The Parasite Load of Trypanosoma cruzi Modulates Feeding and Defecation Patterns of the Chagas Disease Vector Triatoma infestans
por: Chacón, Francisco, et al.
Publicado: (2022)

International Study to Evaluate PCR Methods for Detection of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients
por: Schijman, Alejandro G., et al.
Publicado: (2011)

Analytical validation of real-time quantitative PCR assays for optimum diagnosis of vivax malaria
por: Almeida-de-Oliveira, Natália Ketrin, et al.
Publicado: (2019)

Pentoxifylline Reverses Chronic Experimental Chagasic Cardiomyopathy in Association with Repositioning of Abnormal CD8(+) T-Cell Response
por: Pereira, Isabela Resende, et al.
Publicado: (2015)

Alteration of the aggregation and spatial organization of the vector of Chagas disease, Triatoma infestans, by the parasite Trypanosoma cruzi
por: Depickère, Stéphanie, et al.
Publicado: (2019)

Cannot write session to /tmp/vufind_sessions/sess_j4ihk98rtn7aasm4jq2t0j3cqv