Cargando…

Crystallization and Characterization of an Inflammatory Lectin Purified from the Seeds of Dioclea wilsonii

DwL, a lectin extracted from the seeds of Dioclea wilsonii, is a metalloprotein with strong agglutinating activity against rabbit and ABO erythrocytes, inhibited by glucose and mannose. DwL was purified by affinity chromatography on a Sephadex G-50 column and ion exchange chromatography on a HiTrap...

Descripción completa

Detalles Bibliográficos
Autores principales: Rangel, Thaiz Batista Azevedo, Assreuy, Ana Maria Sampaio, de Freitas Pires, Alana, de Carvalho, Amanda Uliana, Benevides, Raquel Guimarães, da Conceição Simões, Rafael, da Silva, Helton Colares, Bezerra, Maria Júlia Barbosa, do Nascimento, Antonia Samia Fernandes, do Nascimento, Kyria Santiago, Nagano, Celso Shiniti, Sampaio, Alexandre Holanda, Delatorre, Plínio, da Rocha, Bruno Anderson Matias, Fernandes, Patricia Machado Bueno, Cavada, Benildo Sousa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6264520/
https://www.ncbi.nlm.nih.gov/pubmed/21694673
http://dx.doi.org/10.3390/molecules16065087
_version_ 1783375516139520000
author Rangel, Thaiz Batista Azevedo
Assreuy, Ana Maria Sampaio
de Freitas Pires, Alana
de Carvalho, Amanda Uliana
Benevides, Raquel Guimarães
da Conceição Simões, Rafael
da Silva, Helton Colares
Bezerra, Maria Júlia Barbosa
do Nascimento, Antonia Samia Fernandes
do Nascimento, Kyria Santiago
Nagano, Celso Shiniti
Sampaio, Alexandre Holanda
Delatorre, Plínio
da Rocha, Bruno Anderson Matias
Fernandes, Patricia Machado Bueno
Cavada, Benildo Sousa
author_facet Rangel, Thaiz Batista Azevedo
Assreuy, Ana Maria Sampaio
de Freitas Pires, Alana
de Carvalho, Amanda Uliana
Benevides, Raquel Guimarães
da Conceição Simões, Rafael
da Silva, Helton Colares
Bezerra, Maria Júlia Barbosa
do Nascimento, Antonia Samia Fernandes
do Nascimento, Kyria Santiago
Nagano, Celso Shiniti
Sampaio, Alexandre Holanda
Delatorre, Plínio
da Rocha, Bruno Anderson Matias
Fernandes, Patricia Machado Bueno
Cavada, Benildo Sousa
author_sort Rangel, Thaiz Batista Azevedo
collection PubMed
description DwL, a lectin extracted from the seeds of Dioclea wilsonii, is a metalloprotein with strong agglutinating activity against rabbit and ABO erythrocytes, inhibited by glucose and mannose. DwL was purified by affinity chromatography on a Sephadex G-50 column and ion exchange chromatography on a HiTrap SP XL column. SDS-PAGE revealed three electrophoretic bands corresponding to the α (25,634 ± 2 Da), β (12,873 ± 2 Da) and γ (12,779 ± 2 Da) chains. Protein sequencing was done by Tandem Mass Spectrometry. The primary sequence featured 237 amino acids and was highly homologous to other reported Diocleinae lectins. A complete X-ray dataset was collected at 2.0 Å for X-Man-complexed DWL crystals produced by the vapor diffusion method. The crystals were orthorhombic and belonged to the space group I222, with the unit-cell parameters a = 59.6, b = 67.9 and c = 109.0 Å. DWL differed in potency from other ConA-like lectins and was found to induce neutrophil migration in rats, making it particularly useful in structural/functional studies of this class of proteins.
format Online
Article
Text
id pubmed-6264520
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-62645202018-12-10 Crystallization and Characterization of an Inflammatory Lectin Purified from the Seeds of Dioclea wilsonii Rangel, Thaiz Batista Azevedo Assreuy, Ana Maria Sampaio de Freitas Pires, Alana de Carvalho, Amanda Uliana Benevides, Raquel Guimarães da Conceição Simões, Rafael da Silva, Helton Colares Bezerra, Maria Júlia Barbosa do Nascimento, Antonia Samia Fernandes do Nascimento, Kyria Santiago Nagano, Celso Shiniti Sampaio, Alexandre Holanda Delatorre, Plínio da Rocha, Bruno Anderson Matias Fernandes, Patricia Machado Bueno Cavada, Benildo Sousa Molecules Article DwL, a lectin extracted from the seeds of Dioclea wilsonii, is a metalloprotein with strong agglutinating activity against rabbit and ABO erythrocytes, inhibited by glucose and mannose. DwL was purified by affinity chromatography on a Sephadex G-50 column and ion exchange chromatography on a HiTrap SP XL column. SDS-PAGE revealed three electrophoretic bands corresponding to the α (25,634 ± 2 Da), β (12,873 ± 2 Da) and γ (12,779 ± 2 Da) chains. Protein sequencing was done by Tandem Mass Spectrometry. The primary sequence featured 237 amino acids and was highly homologous to other reported Diocleinae lectins. A complete X-ray dataset was collected at 2.0 Å for X-Man-complexed DWL crystals produced by the vapor diffusion method. The crystals were orthorhombic and belonged to the space group I222, with the unit-cell parameters a = 59.6, b = 67.9 and c = 109.0 Å. DWL differed in potency from other ConA-like lectins and was found to induce neutrophil migration in rats, making it particularly useful in structural/functional studies of this class of proteins. MDPI 2011-06-20 /pmc/articles/PMC6264520/ /pubmed/21694673 http://dx.doi.org/10.3390/molecules16065087 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Rangel, Thaiz Batista Azevedo
Assreuy, Ana Maria Sampaio
de Freitas Pires, Alana
de Carvalho, Amanda Uliana
Benevides, Raquel Guimarães
da Conceição Simões, Rafael
da Silva, Helton Colares
Bezerra, Maria Júlia Barbosa
do Nascimento, Antonia Samia Fernandes
do Nascimento, Kyria Santiago
Nagano, Celso Shiniti
Sampaio, Alexandre Holanda
Delatorre, Plínio
da Rocha, Bruno Anderson Matias
Fernandes, Patricia Machado Bueno
Cavada, Benildo Sousa
Crystallization and Characterization of an Inflammatory Lectin Purified from the Seeds of Dioclea wilsonii
title Crystallization and Characterization of an Inflammatory Lectin Purified from the Seeds of Dioclea wilsonii
title_full Crystallization and Characterization of an Inflammatory Lectin Purified from the Seeds of Dioclea wilsonii
title_fullStr Crystallization and Characterization of an Inflammatory Lectin Purified from the Seeds of Dioclea wilsonii
title_full_unstemmed Crystallization and Characterization of an Inflammatory Lectin Purified from the Seeds of Dioclea wilsonii
title_short Crystallization and Characterization of an Inflammatory Lectin Purified from the Seeds of Dioclea wilsonii
title_sort crystallization and characterization of an inflammatory lectin purified from the seeds of dioclea wilsonii
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6264520/
https://www.ncbi.nlm.nih.gov/pubmed/21694673
http://dx.doi.org/10.3390/molecules16065087
work_keys_str_mv AT rangelthaizbatistaazevedo crystallizationandcharacterizationofaninflammatorylectinpurifiedfromtheseedsofdiocleawilsonii
AT assreuyanamariasampaio crystallizationandcharacterizationofaninflammatorylectinpurifiedfromtheseedsofdiocleawilsonii
AT defreitaspiresalana crystallizationandcharacterizationofaninflammatorylectinpurifiedfromtheseedsofdiocleawilsonii
AT decarvalhoamandauliana crystallizationandcharacterizationofaninflammatorylectinpurifiedfromtheseedsofdiocleawilsonii
AT benevidesraquelguimaraes crystallizationandcharacterizationofaninflammatorylectinpurifiedfromtheseedsofdiocleawilsonii
AT daconceicaosimoesrafael crystallizationandcharacterizationofaninflammatorylectinpurifiedfromtheseedsofdiocleawilsonii
AT dasilvaheltoncolares crystallizationandcharacterizationofaninflammatorylectinpurifiedfromtheseedsofdiocleawilsonii
AT bezerramariajuliabarbosa crystallizationandcharacterizationofaninflammatorylectinpurifiedfromtheseedsofdiocleawilsonii
AT donascimentoantoniasamiafernandes crystallizationandcharacterizationofaninflammatorylectinpurifiedfromtheseedsofdiocleawilsonii
AT donascimentokyriasantiago crystallizationandcharacterizationofaninflammatorylectinpurifiedfromtheseedsofdiocleawilsonii
AT naganocelsoshiniti crystallizationandcharacterizationofaninflammatorylectinpurifiedfromtheseedsofdiocleawilsonii
AT sampaioalexandreholanda crystallizationandcharacterizationofaninflammatorylectinpurifiedfromtheseedsofdiocleawilsonii
AT delatorreplinio crystallizationandcharacterizationofaninflammatorylectinpurifiedfromtheseedsofdiocleawilsonii
AT darochabrunoandersonmatias crystallizationandcharacterizationofaninflammatorylectinpurifiedfromtheseedsofdiocleawilsonii
AT fernandespatriciamachadobueno crystallizationandcharacterizationofaninflammatorylectinpurifiedfromtheseedsofdiocleawilsonii
AT cavadabenildosousa crystallizationandcharacterizationofaninflammatorylectinpurifiedfromtheseedsofdiocleawilsonii