Halomonas sp. OKOH—A Marine Bacterium Isolated from the Bottom Sediment of Algoa Bay—Produces a Polysaccharide Bioflocculant: Partial Characterization and Biochemical Analysis of Its Properties

A bioflocculant-producing bacterium isolated from seawater was identified based on 16S rRNA gene nucleotide sequence to have 99% similarity to that of Halomonas sp. Au160H and the nucleotide sequence was deposited as Halomonas sp. OKOH (Genbank accession number is HQ875722). Influences of carbon sou...

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Detalles Bibliográficos
Autores principales: Mabinya, Leonard V., Cosa, Sekelwa, Mkwetshana, Noxolo, Okoh, Anthony I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6264563/
https://www.ncbi.nlm.nih.gov/pubmed/21613977
http://dx.doi.org/10.3390/molecules16064358
Descripción
Sumario:A bioflocculant-producing bacterium isolated from seawater was identified based on 16S rRNA gene nucleotide sequence to have 99% similarity to that of Halomonas sp. Au160H and the nucleotide sequence was deposited as Halomonas sp. OKOH (Genbank accession number is HQ875722). Influences of carbon source, nitrogen source, salt ions and pH on flocculating activity were investigated. The bioflocculant was optimally produced when glucose (87% flocculating activity) and urea (88% flocculating activity) were used as sources of carbon and nitrogen, respectively. Also, initial pH of 7.0 and Ca(2+) supported optimal production of the bioflocculant with flocculating activities of 87% respectively. Chemical analyses revealed the bioflocculant to be a polysaccharide.

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