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Integrated One-Pot Enrichment and Immobilization of Styrene Monooxygenase (StyA) Using SEPABEAD EC-EA and EC-Q1A Anion-Exchange Carriers

A straightforward one-pot procedure combining enrichment and immobilization of recombinantely expressed FADH(2) dependent styrene monooxygenase (StyA) directly from Escherichia coli cell extracts was investigated. Sepabeads EC-EA and EC-Q1A anion-exchange carriers were employed to non-covalently ads...

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Detalles Bibliográficos
Autores principales: Ruinatscha, Reto, Karande, Rohan, Buehler, Katja, Schmid, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6264573/
https://www.ncbi.nlm.nih.gov/pubmed/21769063
http://dx.doi.org/10.3390/molecules16075975