Cargando…
The Effects of Artesunate on the Expression of EGFR and ABCG2 in A549 Human Lung Cancer Cells and a Xenograft Model
Non-small cell lung cancer (NSCLC) is the leading cause of cancer death worldwide. Clinical and laboratory studies have suggested that multi-targeting approaches against neoplastic cells could help to increase patient survival and might reduce the emergence of cells that are resistant to single-targ...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6264700/ https://www.ncbi.nlm.nih.gov/pubmed/22183882 http://dx.doi.org/10.3390/molecules161210556 |
_version_ | 1783375552282886144 |
---|---|
author | Ma, Hu Yao, Quan Zhang, An-Mei Lin, Sheng Wang, Xin-Xin Wu, Lei Sun, Jian-Guo Chen, Zheng-Tang |
author_facet | Ma, Hu Yao, Quan Zhang, An-Mei Lin, Sheng Wang, Xin-Xin Wu, Lei Sun, Jian-Guo Chen, Zheng-Tang |
author_sort | Ma, Hu |
collection | PubMed |
description | Non-small cell lung cancer (NSCLC) is the leading cause of cancer death worldwide. Clinical and laboratory studies have suggested that multi-targeting approaches against neoplastic cells could help to increase patient survival and might reduce the emergence of cells that are resistant to single-target inhibitors. Artesunate (ART) is one of the most potent and rapidly acting antimalarial agents known, and it also exerts a profound cytotoxic activity toward cancer cells and reverses multi-drug resistance. In the present study, we found that artesunate inhibited NSCLC A549 cell growth and proliferation, induced apoptosis and suppressed tumor growth in a dose-dependent manner in A549 cells and a mouse xenograft model. Furthermore, artesunate down-regulated the expression of epidermal growth factor receptor (EGFR), Akt and ATP-binding cassette subfamily G member 2 (ABCG2) at the mRNA and protein levels in vitro and in vivo. In conclusion, artesunate is an effective anti-cancer drug that may enhance the effectiveness of other anticancer drugs and may reverse multi-drug resistance by suppressing the transcription of ABCG2, which inhibits drug efflux. |
format | Online Article Text |
id | pubmed-6264700 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62647002018-12-10 The Effects of Artesunate on the Expression of EGFR and ABCG2 in A549 Human Lung Cancer Cells and a Xenograft Model Ma, Hu Yao, Quan Zhang, An-Mei Lin, Sheng Wang, Xin-Xin Wu, Lei Sun, Jian-Guo Chen, Zheng-Tang Molecules Article Non-small cell lung cancer (NSCLC) is the leading cause of cancer death worldwide. Clinical and laboratory studies have suggested that multi-targeting approaches against neoplastic cells could help to increase patient survival and might reduce the emergence of cells that are resistant to single-target inhibitors. Artesunate (ART) is one of the most potent and rapidly acting antimalarial agents known, and it also exerts a profound cytotoxic activity toward cancer cells and reverses multi-drug resistance. In the present study, we found that artesunate inhibited NSCLC A549 cell growth and proliferation, induced apoptosis and suppressed tumor growth in a dose-dependent manner in A549 cells and a mouse xenograft model. Furthermore, artesunate down-regulated the expression of epidermal growth factor receptor (EGFR), Akt and ATP-binding cassette subfamily G member 2 (ABCG2) at the mRNA and protein levels in vitro and in vivo. In conclusion, artesunate is an effective anti-cancer drug that may enhance the effectiveness of other anticancer drugs and may reverse multi-drug resistance by suppressing the transcription of ABCG2, which inhibits drug efflux. MDPI 2011-12-19 /pmc/articles/PMC6264700/ /pubmed/22183882 http://dx.doi.org/10.3390/molecules161210556 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Ma, Hu Yao, Quan Zhang, An-Mei Lin, Sheng Wang, Xin-Xin Wu, Lei Sun, Jian-Guo Chen, Zheng-Tang The Effects of Artesunate on the Expression of EGFR and ABCG2 in A549 Human Lung Cancer Cells and a Xenograft Model |
title | The Effects of Artesunate on the Expression of EGFR and ABCG2 in A549 Human Lung Cancer Cells and a Xenograft Model |
title_full | The Effects of Artesunate on the Expression of EGFR and ABCG2 in A549 Human Lung Cancer Cells and a Xenograft Model |
title_fullStr | The Effects of Artesunate on the Expression of EGFR and ABCG2 in A549 Human Lung Cancer Cells and a Xenograft Model |
title_full_unstemmed | The Effects of Artesunate on the Expression of EGFR and ABCG2 in A549 Human Lung Cancer Cells and a Xenograft Model |
title_short | The Effects of Artesunate on the Expression of EGFR and ABCG2 in A549 Human Lung Cancer Cells and a Xenograft Model |
title_sort | effects of artesunate on the expression of egfr and abcg2 in a549 human lung cancer cells and a xenograft model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6264700/ https://www.ncbi.nlm.nih.gov/pubmed/22183882 http://dx.doi.org/10.3390/molecules161210556 |
work_keys_str_mv | AT mahu theeffectsofartesunateontheexpressionofegfrandabcg2ina549humanlungcancercellsandaxenograftmodel AT yaoquan theeffectsofartesunateontheexpressionofegfrandabcg2ina549humanlungcancercellsandaxenograftmodel AT zhanganmei theeffectsofartesunateontheexpressionofegfrandabcg2ina549humanlungcancercellsandaxenograftmodel AT linsheng theeffectsofartesunateontheexpressionofegfrandabcg2ina549humanlungcancercellsandaxenograftmodel AT wangxinxin theeffectsofartesunateontheexpressionofegfrandabcg2ina549humanlungcancercellsandaxenograftmodel AT wulei theeffectsofartesunateontheexpressionofegfrandabcg2ina549humanlungcancercellsandaxenograftmodel AT sunjianguo theeffectsofartesunateontheexpressionofegfrandabcg2ina549humanlungcancercellsandaxenograftmodel AT chenzhengtang theeffectsofartesunateontheexpressionofegfrandabcg2ina549humanlungcancercellsandaxenograftmodel AT mahu effectsofartesunateontheexpressionofegfrandabcg2ina549humanlungcancercellsandaxenograftmodel AT yaoquan effectsofartesunateontheexpressionofegfrandabcg2ina549humanlungcancercellsandaxenograftmodel AT zhanganmei effectsofartesunateontheexpressionofegfrandabcg2ina549humanlungcancercellsandaxenograftmodel AT linsheng effectsofartesunateontheexpressionofegfrandabcg2ina549humanlungcancercellsandaxenograftmodel AT wangxinxin effectsofartesunateontheexpressionofegfrandabcg2ina549humanlungcancercellsandaxenograftmodel AT wulei effectsofartesunateontheexpressionofegfrandabcg2ina549humanlungcancercellsandaxenograftmodel AT sunjianguo effectsofartesunateontheexpressionofegfrandabcg2ina549humanlungcancercellsandaxenograftmodel AT chenzhengtang effectsofartesunateontheexpressionofegfrandabcg2ina549humanlungcancercellsandaxenograftmodel |