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The Effects of Artesunate on the Expression of EGFR and ABCG2 in A549 Human Lung Cancer Cells and a Xenograft Model

Non-small cell lung cancer (NSCLC) is the leading cause of cancer death worldwide. Clinical and laboratory studies have suggested that multi-targeting approaches against neoplastic cells could help to increase patient survival and might reduce the emergence of cells that are resistant to single-targ...

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Autores principales: Ma, Hu, Yao, Quan, Zhang, An-Mei, Lin, Sheng, Wang, Xin-Xin, Wu, Lei, Sun, Jian-Guo, Chen, Zheng-Tang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6264700/
https://www.ncbi.nlm.nih.gov/pubmed/22183882
http://dx.doi.org/10.3390/molecules161210556
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author Ma, Hu
Yao, Quan
Zhang, An-Mei
Lin, Sheng
Wang, Xin-Xin
Wu, Lei
Sun, Jian-Guo
Chen, Zheng-Tang
author_facet Ma, Hu
Yao, Quan
Zhang, An-Mei
Lin, Sheng
Wang, Xin-Xin
Wu, Lei
Sun, Jian-Guo
Chen, Zheng-Tang
author_sort Ma, Hu
collection PubMed
description Non-small cell lung cancer (NSCLC) is the leading cause of cancer death worldwide. Clinical and laboratory studies have suggested that multi-targeting approaches against neoplastic cells could help to increase patient survival and might reduce the emergence of cells that are resistant to single-target inhibitors. Artesunate (ART) is one of the most potent and rapidly acting antimalarial agents known, and it also exerts a profound cytotoxic activity toward cancer cells and reverses multi-drug resistance. In the present study, we found that artesunate inhibited NSCLC A549 cell growth and proliferation, induced apoptosis and suppressed tumor growth in a dose-dependent manner in A549 cells and a mouse xenograft model. Furthermore, artesunate down-regulated the expression of epidermal growth factor receptor (EGFR), Akt and ATP-binding cassette subfamily G member 2 (ABCG2) at the mRNA and protein levels in vitro and in vivo. In conclusion, artesunate is an effective anti-cancer drug that may enhance the effectiveness of other anticancer drugs and may reverse multi-drug resistance by suppressing the transcription of ABCG2, which inhibits drug efflux.
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spelling pubmed-62647002018-12-10 The Effects of Artesunate on the Expression of EGFR and ABCG2 in A549 Human Lung Cancer Cells and a Xenograft Model Ma, Hu Yao, Quan Zhang, An-Mei Lin, Sheng Wang, Xin-Xin Wu, Lei Sun, Jian-Guo Chen, Zheng-Tang Molecules Article Non-small cell lung cancer (NSCLC) is the leading cause of cancer death worldwide. Clinical and laboratory studies have suggested that multi-targeting approaches against neoplastic cells could help to increase patient survival and might reduce the emergence of cells that are resistant to single-target inhibitors. Artesunate (ART) is one of the most potent and rapidly acting antimalarial agents known, and it also exerts a profound cytotoxic activity toward cancer cells and reverses multi-drug resistance. In the present study, we found that artesunate inhibited NSCLC A549 cell growth and proliferation, induced apoptosis and suppressed tumor growth in a dose-dependent manner in A549 cells and a mouse xenograft model. Furthermore, artesunate down-regulated the expression of epidermal growth factor receptor (EGFR), Akt and ATP-binding cassette subfamily G member 2 (ABCG2) at the mRNA and protein levels in vitro and in vivo. In conclusion, artesunate is an effective anti-cancer drug that may enhance the effectiveness of other anticancer drugs and may reverse multi-drug resistance by suppressing the transcription of ABCG2, which inhibits drug efflux. MDPI 2011-12-19 /pmc/articles/PMC6264700/ /pubmed/22183882 http://dx.doi.org/10.3390/molecules161210556 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Ma, Hu
Yao, Quan
Zhang, An-Mei
Lin, Sheng
Wang, Xin-Xin
Wu, Lei
Sun, Jian-Guo
Chen, Zheng-Tang
The Effects of Artesunate on the Expression of EGFR and ABCG2 in A549 Human Lung Cancer Cells and a Xenograft Model
title The Effects of Artesunate on the Expression of EGFR and ABCG2 in A549 Human Lung Cancer Cells and a Xenograft Model
title_full The Effects of Artesunate on the Expression of EGFR and ABCG2 in A549 Human Lung Cancer Cells and a Xenograft Model
title_fullStr The Effects of Artesunate on the Expression of EGFR and ABCG2 in A549 Human Lung Cancer Cells and a Xenograft Model
title_full_unstemmed The Effects of Artesunate on the Expression of EGFR and ABCG2 in A549 Human Lung Cancer Cells and a Xenograft Model
title_short The Effects of Artesunate on the Expression of EGFR and ABCG2 in A549 Human Lung Cancer Cells and a Xenograft Model
title_sort effects of artesunate on the expression of egfr and abcg2 in a549 human lung cancer cells and a xenograft model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6264700/
https://www.ncbi.nlm.nih.gov/pubmed/22183882
http://dx.doi.org/10.3390/molecules161210556
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