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In silico prediction of monovalent and chimeric tetravalent vaccines for prevention and treatment of dengue fever
Reverse vaccinology method was used to predict the monovalent peptide vaccine candidate to produce antibodies for therapeutic purpose and to predict tetravalent vaccine candidate to act as a common vaccine to cover all the dengue virus serotypes. Envelope (E)-proteins of DENV-1-4 serotypes were used...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Editorial Department of Journal of Biomedical Research
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6265401/ https://www.ncbi.nlm.nih.gov/pubmed/29497025 http://dx.doi.org/10.7555/JBR.31.20160109 |
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author | Subramaniyan, Vijayakumar Venkatachalam, Ramesh Srinivasan, Prabhu Palani, Manogar |
author_facet | Subramaniyan, Vijayakumar Venkatachalam, Ramesh Srinivasan, Prabhu Palani, Manogar |
author_sort | Subramaniyan, Vijayakumar |
collection | PubMed |
description | Reverse vaccinology method was used to predict the monovalent peptide vaccine candidate to produce antibodies for therapeutic purpose and to predict tetravalent vaccine candidate to act as a common vaccine to cover all the dengue virus serotypes. Envelope (E)-proteins of DENV-1-4 serotypes were used for vaccine prediction using NCBI, Uniprot/Swissprot, Swiss-prot viewer, VaxiJen V2.0, TMHMM, BCPREDS, Propred-1, Propred and MHC Pred. E-proteins of DENV-1-4 serotypes were identified as antigen from which T cell epitopes, through B cell epitopes, were predicted to act as peptide vaccine candidates. Each selected T cell epitope of E-protein was confirmed to act as vaccine and to induce complementary antibody against particular serotype of dengue virus. Chimeric tetravalent vaccine was formed by the conjugation of four vaccines, each from four dengue serotypes to act as a common vaccine candidate for all the four dengue serotypes. It can be justifiably concluded that the monovalent 9-mer T cell epitope for each DENV serotype can be used to produce specific antibody against dengue virus and a chimeric common tetravalent vaccine candidate to yield a comparative vaccine to cover any of the four dengue virus serotype. This vaccine is expected to be highly immunogenic against dengue fever. |
format | Online Article Text |
id | pubmed-6265401 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Editorial Department of Journal of Biomedical Research |
record_format | MEDLINE/PubMed |
spelling | pubmed-62654012018-12-11 In silico prediction of monovalent and chimeric tetravalent vaccines for prevention and treatment of dengue fever Subramaniyan, Vijayakumar Venkatachalam, Ramesh Srinivasan, Prabhu Palani, Manogar J Biomed Res Original Article Reverse vaccinology method was used to predict the monovalent peptide vaccine candidate to produce antibodies for therapeutic purpose and to predict tetravalent vaccine candidate to act as a common vaccine to cover all the dengue virus serotypes. Envelope (E)-proteins of DENV-1-4 serotypes were used for vaccine prediction using NCBI, Uniprot/Swissprot, Swiss-prot viewer, VaxiJen V2.0, TMHMM, BCPREDS, Propred-1, Propred and MHC Pred. E-proteins of DENV-1-4 serotypes were identified as antigen from which T cell epitopes, through B cell epitopes, were predicted to act as peptide vaccine candidates. Each selected T cell epitope of E-protein was confirmed to act as vaccine and to induce complementary antibody against particular serotype of dengue virus. Chimeric tetravalent vaccine was formed by the conjugation of four vaccines, each from four dengue serotypes to act as a common vaccine candidate for all the four dengue serotypes. It can be justifiably concluded that the monovalent 9-mer T cell epitope for each DENV serotype can be used to produce specific antibody against dengue virus and a chimeric common tetravalent vaccine candidate to yield a comparative vaccine to cover any of the four dengue virus serotype. This vaccine is expected to be highly immunogenic against dengue fever. Editorial Department of Journal of Biomedical Research 2018-06-26 2017-12-02 /pmc/articles/PMC6265401/ /pubmed/29497025 http://dx.doi.org/10.7555/JBR.31.20160109 Text en © 2018 by the Journal of Biomedical Research. All rights reserved /creativecommons.org/licenses/by/4.0/ This is an open access article under the Creative Commons Attribution (CC BY 4.0) license, which permits others to distribute, remix, adapt and build upon this work, for commercial use, provided the original work is properly cited. |
spellingShingle | Original Article Subramaniyan, Vijayakumar Venkatachalam, Ramesh Srinivasan, Prabhu Palani, Manogar In silico prediction of monovalent and chimeric tetravalent vaccines for prevention and treatment of dengue fever |
title | In silico prediction of monovalent and chimeric tetravalent vaccines for prevention and treatment of dengue fever |
title_full | In silico prediction of monovalent and chimeric tetravalent vaccines for prevention and treatment of dengue fever |
title_fullStr | In silico prediction of monovalent and chimeric tetravalent vaccines for prevention and treatment of dengue fever |
title_full_unstemmed | In silico prediction of monovalent and chimeric tetravalent vaccines for prevention and treatment of dengue fever |
title_short | In silico prediction of monovalent and chimeric tetravalent vaccines for prevention and treatment of dengue fever |
title_sort | in silico prediction of monovalent and chimeric tetravalent vaccines for prevention and treatment of dengue fever |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6265401/ https://www.ncbi.nlm.nih.gov/pubmed/29497025 http://dx.doi.org/10.7555/JBR.31.20160109 |
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