Characterization and in vitro data of antibody drug conjugates (ADCs) derived from heterotrifunctional linker designed for the site-specific preparation of dual ADCs

Experimental procedures and (1)H and (13)C NMR of the heterotrifunctional linker used for preparation of dual drug conjugates and PBD payload are included. Procedure for carrying preparation of antibody linker conjugate via thiol maleimide conjugation and antibody drug conjugates (ADCs) using copper...

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Autores principales: Kumar, Amit, Kinneer, Krista, Masterson, Luke, Ezeadi, Ebele, Howard, Philip, Wu, Herren, Gao, Changshou, Dimasi, Nazzareno
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6265423/
https://www.ncbi.nlm.nih.gov/pubmed/30533469
http://dx.doi.org/10.1016/j.dib.2018.11.005
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author Kumar, Amit
Kinneer, Krista
Masterson, Luke
Ezeadi, Ebele
Howard, Philip
Wu, Herren
Gao, Changshou
Dimasi, Nazzareno
author_facet Kumar, Amit
Kinneer, Krista
Masterson, Luke
Ezeadi, Ebele
Howard, Philip
Wu, Herren
Gao, Changshou
Dimasi, Nazzareno
author_sort Kumar, Amit
collection PubMed
description Experimental procedures and (1)H and (13)C NMR of the heterotrifunctional linker used for preparation of dual drug conjugates and PBD payload are included. Procedure for carrying preparation of antibody linker conjugate via thiol maleimide conjugation and antibody drug conjugates (ADCs) using copper assisted click reaction and oxime ligation, their cell viability assay and western blotting procedures of the resultant conjugates are detailed. Also, reduced mass spectroscopy results and in vitro cytotoxicity of antibody drug conjugates used in this article are shown.
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spelling pubmed-62654232018-12-07 Characterization and in vitro data of antibody drug conjugates (ADCs) derived from heterotrifunctional linker designed for the site-specific preparation of dual ADCs Kumar, Amit Kinneer, Krista Masterson, Luke Ezeadi, Ebele Howard, Philip Wu, Herren Gao, Changshou Dimasi, Nazzareno Data Brief Chemistry Experimental procedures and (1)H and (13)C NMR of the heterotrifunctional linker used for preparation of dual drug conjugates and PBD payload are included. Procedure for carrying preparation of antibody linker conjugate via thiol maleimide conjugation and antibody drug conjugates (ADCs) using copper assisted click reaction and oxime ligation, their cell viability assay and western blotting procedures of the resultant conjugates are detailed. Also, reduced mass spectroscopy results and in vitro cytotoxicity of antibody drug conjugates used in this article are shown. Elsevier 2018-11-06 /pmc/articles/PMC6265423/ /pubmed/30533469 http://dx.doi.org/10.1016/j.dib.2018.11.005 Text en © 2018 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Chemistry
Kumar, Amit
Kinneer, Krista
Masterson, Luke
Ezeadi, Ebele
Howard, Philip
Wu, Herren
Gao, Changshou
Dimasi, Nazzareno
Characterization and in vitro data of antibody drug conjugates (ADCs) derived from heterotrifunctional linker designed for the site-specific preparation of dual ADCs
title Characterization and in vitro data of antibody drug conjugates (ADCs) derived from heterotrifunctional linker designed for the site-specific preparation of dual ADCs
title_full Characterization and in vitro data of antibody drug conjugates (ADCs) derived from heterotrifunctional linker designed for the site-specific preparation of dual ADCs
title_fullStr Characterization and in vitro data of antibody drug conjugates (ADCs) derived from heterotrifunctional linker designed for the site-specific preparation of dual ADCs
title_full_unstemmed Characterization and in vitro data of antibody drug conjugates (ADCs) derived from heterotrifunctional linker designed for the site-specific preparation of dual ADCs
title_short Characterization and in vitro data of antibody drug conjugates (ADCs) derived from heterotrifunctional linker designed for the site-specific preparation of dual ADCs
title_sort characterization and in vitro data of antibody drug conjugates (adcs) derived from heterotrifunctional linker designed for the site-specific preparation of dual adcs
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6265423/
https://www.ncbi.nlm.nih.gov/pubmed/30533469
http://dx.doi.org/10.1016/j.dib.2018.11.005
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