Cargando…

Human Bocavirus Infection Markers in Peripheral Blood and Stool Samples of Children with Acute Gastroenteritis

Human bocaviruses (HBoVs) 1–4 belong to the Parvoviridae family, and they infect the respiratory or gastrointestinal tracts in children. We investigated the prevalence of HBoV1–4 DNAs in the blood and stool samples, and of HBoV1–4 IgG and IgM in the plasma samples, of children presenting with acute...

Descripción completa

Detalles Bibliográficos
Autores principales: Nora-Krukle, Zaiga, Vilmane, Anda, Xu, Man, Rasa, Santa, Ziemele, Inga, Silina, Elina, Söderlund-Venermo, Maria, Gardovska, Dace, Murovska, Modra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6265904/
https://www.ncbi.nlm.nih.gov/pubmed/30445732
http://dx.doi.org/10.3390/v10110639
_version_ 1783375720633860096
author Nora-Krukle, Zaiga
Vilmane, Anda
Xu, Man
Rasa, Santa
Ziemele, Inga
Silina, Elina
Söderlund-Venermo, Maria
Gardovska, Dace
Murovska, Modra
author_facet Nora-Krukle, Zaiga
Vilmane, Anda
Xu, Man
Rasa, Santa
Ziemele, Inga
Silina, Elina
Söderlund-Venermo, Maria
Gardovska, Dace
Murovska, Modra
author_sort Nora-Krukle, Zaiga
collection PubMed
description Human bocaviruses (HBoVs) 1–4 belong to the Parvoviridae family, and they infect the respiratory or gastrointestinal tracts in children. We investigated the prevalence of HBoV1–4 DNAs in the blood and stool samples, and of HBoV1–4 IgG and IgM in the plasma samples, of children presenting with acute gastroenteritis (AGE). In addition, we identified HBoV co-infections with the five most frequent gastrointestinal pathogens. A total of 83 paired blood and stool samples were collected from children aged five years or less. Infection markers of HBoV1, 2, or 3 (viral DNA in blood and/or stool and/or antibodies) were detected in 61 out of 83 (73.5%) patients. HBoV1, 2, or 3 DNA as a monoinfection was revealed in 18.1%, 2.4%, and 1.2%, respectively, and 21.7% in total. In 56.1% of the HBoV DNA-positive patients, the presence in stool of another virus—most frequently norovirus or rotavirus—was observed. In conclusion, this study, for the first time, illustrates the prevalence and genetic diversity of HBoVs in Latvian children with gastroenteritis, and shows a widespread distribution of these viruses in the community. HBoV1 and 2 are commonly found as single infectious agents in children with AGE, suggesting that the viruses can be as pathogenic by themselves as other enteric agents are.
format Online
Article
Text
id pubmed-6265904
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-62659042018-12-07 Human Bocavirus Infection Markers in Peripheral Blood and Stool Samples of Children with Acute Gastroenteritis Nora-Krukle, Zaiga Vilmane, Anda Xu, Man Rasa, Santa Ziemele, Inga Silina, Elina Söderlund-Venermo, Maria Gardovska, Dace Murovska, Modra Viruses Article Human bocaviruses (HBoVs) 1–4 belong to the Parvoviridae family, and they infect the respiratory or gastrointestinal tracts in children. We investigated the prevalence of HBoV1–4 DNAs in the blood and stool samples, and of HBoV1–4 IgG and IgM in the plasma samples, of children presenting with acute gastroenteritis (AGE). In addition, we identified HBoV co-infections with the five most frequent gastrointestinal pathogens. A total of 83 paired blood and stool samples were collected from children aged five years or less. Infection markers of HBoV1, 2, or 3 (viral DNA in blood and/or stool and/or antibodies) were detected in 61 out of 83 (73.5%) patients. HBoV1, 2, or 3 DNA as a monoinfection was revealed in 18.1%, 2.4%, and 1.2%, respectively, and 21.7% in total. In 56.1% of the HBoV DNA-positive patients, the presence in stool of another virus—most frequently norovirus or rotavirus—was observed. In conclusion, this study, for the first time, illustrates the prevalence and genetic diversity of HBoVs in Latvian children with gastroenteritis, and shows a widespread distribution of these viruses in the community. HBoV1 and 2 are commonly found as single infectious agents in children with AGE, suggesting that the viruses can be as pathogenic by themselves as other enteric agents are. MDPI 2018-11-15 /pmc/articles/PMC6265904/ /pubmed/30445732 http://dx.doi.org/10.3390/v10110639 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nora-Krukle, Zaiga
Vilmane, Anda
Xu, Man
Rasa, Santa
Ziemele, Inga
Silina, Elina
Söderlund-Venermo, Maria
Gardovska, Dace
Murovska, Modra
Human Bocavirus Infection Markers in Peripheral Blood and Stool Samples of Children with Acute Gastroenteritis
title Human Bocavirus Infection Markers in Peripheral Blood and Stool Samples of Children with Acute Gastroenteritis
title_full Human Bocavirus Infection Markers in Peripheral Blood and Stool Samples of Children with Acute Gastroenteritis
title_fullStr Human Bocavirus Infection Markers in Peripheral Blood and Stool Samples of Children with Acute Gastroenteritis
title_full_unstemmed Human Bocavirus Infection Markers in Peripheral Blood and Stool Samples of Children with Acute Gastroenteritis
title_short Human Bocavirus Infection Markers in Peripheral Blood and Stool Samples of Children with Acute Gastroenteritis
title_sort human bocavirus infection markers in peripheral blood and stool samples of children with acute gastroenteritis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6265904/
https://www.ncbi.nlm.nih.gov/pubmed/30445732
http://dx.doi.org/10.3390/v10110639
work_keys_str_mv AT norakruklezaiga humanbocavirusinfectionmarkersinperipheralbloodandstoolsamplesofchildrenwithacutegastroenteritis
AT vilmaneanda humanbocavirusinfectionmarkersinperipheralbloodandstoolsamplesofchildrenwithacutegastroenteritis
AT xuman humanbocavirusinfectionmarkersinperipheralbloodandstoolsamplesofchildrenwithacutegastroenteritis
AT rasasanta humanbocavirusinfectionmarkersinperipheralbloodandstoolsamplesofchildrenwithacutegastroenteritis
AT ziemeleinga humanbocavirusinfectionmarkersinperipheralbloodandstoolsamplesofchildrenwithacutegastroenteritis
AT silinaelina humanbocavirusinfectionmarkersinperipheralbloodandstoolsamplesofchildrenwithacutegastroenteritis
AT soderlundvenermomaria humanbocavirusinfectionmarkersinperipheralbloodandstoolsamplesofchildrenwithacutegastroenteritis
AT gardovskadace humanbocavirusinfectionmarkersinperipheralbloodandstoolsamplesofchildrenwithacutegastroenteritis
AT murovskamodra humanbocavirusinfectionmarkersinperipheralbloodandstoolsamplesofchildrenwithacutegastroenteritis