Cargando…

Internal Structure of Matrix-Type Multilayer Capsules Templated on Porous Vaterite CaCO(3) Crystals as Probed by Staining with a Fluorescence Dye

Multilayer capsules templated on decomposable vaterite CaCO(3) crystals are widely used as vehicles for drug delivery. The capsule represents typically not a hollow but matrix-like structure due to polymer diffusion into the porous crystals during multilayer deposition. The capsule formation mechani...

Descripción completa

Detalles Bibliográficos
Autores principales: Jeannot, Lucas, Bell, Michael, Ashwell, Ryan, Volodkin, Dmitry, Vikulina, Anna S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6265917/
https://www.ncbi.nlm.nih.gov/pubmed/30715046
http://dx.doi.org/10.3390/mi9110547
_version_ 1783375723647467520
author Jeannot, Lucas
Bell, Michael
Ashwell, Ryan
Volodkin, Dmitry
Vikulina, Anna S.
author_facet Jeannot, Lucas
Bell, Michael
Ashwell, Ryan
Volodkin, Dmitry
Vikulina, Anna S.
author_sort Jeannot, Lucas
collection PubMed
description Multilayer capsules templated on decomposable vaterite CaCO(3) crystals are widely used as vehicles for drug delivery. The capsule represents typically not a hollow but matrix-like structure due to polymer diffusion into the porous crystals during multilayer deposition. The capsule formation mechanism is not well-studied but its understanding is crucial to tune capsule structure for a proper drug release performance. This study proposes new approach to noninvasively probe and adjust internal capsule structure. Polymer capsules made of poly(styrene-sulfonate) (PSS) and poly(diallyldimethylammonium chloride) (PDAD) have been stained with fluorescence dye rhodamine 6G. Physical-chemical aspects of intermolecular interactions required to validate the approach and adjust capsule structure are addressed. The capsules consist of a defined shell (typically 0.5–2 µm) and an internal matrix of PSS-PDAD complex (typically 10–40% of a total capsule volume). An increase of ionic strength and polymer deposition time leads to the thickening of the capsule shell and formation of a denser internal matrix, respectively. This is explained by effects of a polymer conformation and limitations in polymer diffusion through the crystal pores. We believe that the design of the capsules with desired internal structure will allow achieving effective encapsulation and controlled/programmed release of bioactives for advanced drug delivery applications.
format Online
Article
Text
id pubmed-6265917
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-62659172018-12-06 Internal Structure of Matrix-Type Multilayer Capsules Templated on Porous Vaterite CaCO(3) Crystals as Probed by Staining with a Fluorescence Dye Jeannot, Lucas Bell, Michael Ashwell, Ryan Volodkin, Dmitry Vikulina, Anna S. Micromachines (Basel) Article Multilayer capsules templated on decomposable vaterite CaCO(3) crystals are widely used as vehicles for drug delivery. The capsule represents typically not a hollow but matrix-like structure due to polymer diffusion into the porous crystals during multilayer deposition. The capsule formation mechanism is not well-studied but its understanding is crucial to tune capsule structure for a proper drug release performance. This study proposes new approach to noninvasively probe and adjust internal capsule structure. Polymer capsules made of poly(styrene-sulfonate) (PSS) and poly(diallyldimethylammonium chloride) (PDAD) have been stained with fluorescence dye rhodamine 6G. Physical-chemical aspects of intermolecular interactions required to validate the approach and adjust capsule structure are addressed. The capsules consist of a defined shell (typically 0.5–2 µm) and an internal matrix of PSS-PDAD complex (typically 10–40% of a total capsule volume). An increase of ionic strength and polymer deposition time leads to the thickening of the capsule shell and formation of a denser internal matrix, respectively. This is explained by effects of a polymer conformation and limitations in polymer diffusion through the crystal pores. We believe that the design of the capsules with desired internal structure will allow achieving effective encapsulation and controlled/programmed release of bioactives for advanced drug delivery applications. MDPI 2018-10-25 /pmc/articles/PMC6265917/ /pubmed/30715046 http://dx.doi.org/10.3390/mi9110547 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jeannot, Lucas
Bell, Michael
Ashwell, Ryan
Volodkin, Dmitry
Vikulina, Anna S.
Internal Structure of Matrix-Type Multilayer Capsules Templated on Porous Vaterite CaCO(3) Crystals as Probed by Staining with a Fluorescence Dye
title Internal Structure of Matrix-Type Multilayer Capsules Templated on Porous Vaterite CaCO(3) Crystals as Probed by Staining with a Fluorescence Dye
title_full Internal Structure of Matrix-Type Multilayer Capsules Templated on Porous Vaterite CaCO(3) Crystals as Probed by Staining with a Fluorescence Dye
title_fullStr Internal Structure of Matrix-Type Multilayer Capsules Templated on Porous Vaterite CaCO(3) Crystals as Probed by Staining with a Fluorescence Dye
title_full_unstemmed Internal Structure of Matrix-Type Multilayer Capsules Templated on Porous Vaterite CaCO(3) Crystals as Probed by Staining with a Fluorescence Dye
title_short Internal Structure of Matrix-Type Multilayer Capsules Templated on Porous Vaterite CaCO(3) Crystals as Probed by Staining with a Fluorescence Dye
title_sort internal structure of matrix-type multilayer capsules templated on porous vaterite caco(3) crystals as probed by staining with a fluorescence dye
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6265917/
https://www.ncbi.nlm.nih.gov/pubmed/30715046
http://dx.doi.org/10.3390/mi9110547
work_keys_str_mv AT jeannotlucas internalstructureofmatrixtypemultilayercapsulestemplatedonporousvateritecaco3crystalsasprobedbystainingwithafluorescencedye
AT bellmichael internalstructureofmatrixtypemultilayercapsulestemplatedonporousvateritecaco3crystalsasprobedbystainingwithafluorescencedye
AT ashwellryan internalstructureofmatrixtypemultilayercapsulestemplatedonporousvateritecaco3crystalsasprobedbystainingwithafluorescencedye
AT volodkindmitry internalstructureofmatrixtypemultilayercapsulestemplatedonporousvateritecaco3crystalsasprobedbystainingwithafluorescencedye
AT vikulinaannas internalstructureofmatrixtypemultilayercapsulestemplatedonporousvateritecaco3crystalsasprobedbystainingwithafluorescencedye