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Internal Structure of Matrix-Type Multilayer Capsules Templated on Porous Vaterite CaCO(3) Crystals as Probed by Staining with a Fluorescence Dye
Multilayer capsules templated on decomposable vaterite CaCO(3) crystals are widely used as vehicles for drug delivery. The capsule represents typically not a hollow but matrix-like structure due to polymer diffusion into the porous crystals during multilayer deposition. The capsule formation mechani...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6265917/ https://www.ncbi.nlm.nih.gov/pubmed/30715046 http://dx.doi.org/10.3390/mi9110547 |
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author | Jeannot, Lucas Bell, Michael Ashwell, Ryan Volodkin, Dmitry Vikulina, Anna S. |
author_facet | Jeannot, Lucas Bell, Michael Ashwell, Ryan Volodkin, Dmitry Vikulina, Anna S. |
author_sort | Jeannot, Lucas |
collection | PubMed |
description | Multilayer capsules templated on decomposable vaterite CaCO(3) crystals are widely used as vehicles for drug delivery. The capsule represents typically not a hollow but matrix-like structure due to polymer diffusion into the porous crystals during multilayer deposition. The capsule formation mechanism is not well-studied but its understanding is crucial to tune capsule structure for a proper drug release performance. This study proposes new approach to noninvasively probe and adjust internal capsule structure. Polymer capsules made of poly(styrene-sulfonate) (PSS) and poly(diallyldimethylammonium chloride) (PDAD) have been stained with fluorescence dye rhodamine 6G. Physical-chemical aspects of intermolecular interactions required to validate the approach and adjust capsule structure are addressed. The capsules consist of a defined shell (typically 0.5–2 µm) and an internal matrix of PSS-PDAD complex (typically 10–40% of a total capsule volume). An increase of ionic strength and polymer deposition time leads to the thickening of the capsule shell and formation of a denser internal matrix, respectively. This is explained by effects of a polymer conformation and limitations in polymer diffusion through the crystal pores. We believe that the design of the capsules with desired internal structure will allow achieving effective encapsulation and controlled/programmed release of bioactives for advanced drug delivery applications. |
format | Online Article Text |
id | pubmed-6265917 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62659172018-12-06 Internal Structure of Matrix-Type Multilayer Capsules Templated on Porous Vaterite CaCO(3) Crystals as Probed by Staining with a Fluorescence Dye Jeannot, Lucas Bell, Michael Ashwell, Ryan Volodkin, Dmitry Vikulina, Anna S. Micromachines (Basel) Article Multilayer capsules templated on decomposable vaterite CaCO(3) crystals are widely used as vehicles for drug delivery. The capsule represents typically not a hollow but matrix-like structure due to polymer diffusion into the porous crystals during multilayer deposition. The capsule formation mechanism is not well-studied but its understanding is crucial to tune capsule structure for a proper drug release performance. This study proposes new approach to noninvasively probe and adjust internal capsule structure. Polymer capsules made of poly(styrene-sulfonate) (PSS) and poly(diallyldimethylammonium chloride) (PDAD) have been stained with fluorescence dye rhodamine 6G. Physical-chemical aspects of intermolecular interactions required to validate the approach and adjust capsule structure are addressed. The capsules consist of a defined shell (typically 0.5–2 µm) and an internal matrix of PSS-PDAD complex (typically 10–40% of a total capsule volume). An increase of ionic strength and polymer deposition time leads to the thickening of the capsule shell and formation of a denser internal matrix, respectively. This is explained by effects of a polymer conformation and limitations in polymer diffusion through the crystal pores. We believe that the design of the capsules with desired internal structure will allow achieving effective encapsulation and controlled/programmed release of bioactives for advanced drug delivery applications. MDPI 2018-10-25 /pmc/articles/PMC6265917/ /pubmed/30715046 http://dx.doi.org/10.3390/mi9110547 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Jeannot, Lucas Bell, Michael Ashwell, Ryan Volodkin, Dmitry Vikulina, Anna S. Internal Structure of Matrix-Type Multilayer Capsules Templated on Porous Vaterite CaCO(3) Crystals as Probed by Staining with a Fluorescence Dye |
title | Internal Structure of Matrix-Type Multilayer Capsules Templated on Porous Vaterite CaCO(3) Crystals as Probed by Staining with a Fluorescence Dye |
title_full | Internal Structure of Matrix-Type Multilayer Capsules Templated on Porous Vaterite CaCO(3) Crystals as Probed by Staining with a Fluorescence Dye |
title_fullStr | Internal Structure of Matrix-Type Multilayer Capsules Templated on Porous Vaterite CaCO(3) Crystals as Probed by Staining with a Fluorescence Dye |
title_full_unstemmed | Internal Structure of Matrix-Type Multilayer Capsules Templated on Porous Vaterite CaCO(3) Crystals as Probed by Staining with a Fluorescence Dye |
title_short | Internal Structure of Matrix-Type Multilayer Capsules Templated on Porous Vaterite CaCO(3) Crystals as Probed by Staining with a Fluorescence Dye |
title_sort | internal structure of matrix-type multilayer capsules templated on porous vaterite caco(3) crystals as probed by staining with a fluorescence dye |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6265917/ https://www.ncbi.nlm.nih.gov/pubmed/30715046 http://dx.doi.org/10.3390/mi9110547 |
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