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Pantoea Bacteriophage vB_PagS_Vid5: A Low-Temperature Siphovirus That Harbors a Cluster of Genes Involved in the Biosynthesis of Archaeosine
A novel low-temperature siphovirus, vB_PagS_Vid5 (Vid5), was isolated in Lithuania using Pantoea agglomerans isolate for the phage propagation. The 61,437 bp genome of Vid5 has a G–C content of 48.8% and contains 99 probable protein encoding genes and one gene for tRNA(Ser). A comparative sequence a...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6266253/ https://www.ncbi.nlm.nih.gov/pubmed/30366363 http://dx.doi.org/10.3390/v10110583 |
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author | Šimoliūnas, Eugenijus Šimoliūnienė, Monika Kaliniene, Laura Zajančkauskaitė, Aurelija Skapas, Martynas Meškys, Rolandas Kaupinis, Algirdas Valius, Mindaugas Truncaitė, Lidija |
author_facet | Šimoliūnas, Eugenijus Šimoliūnienė, Monika Kaliniene, Laura Zajančkauskaitė, Aurelija Skapas, Martynas Meškys, Rolandas Kaupinis, Algirdas Valius, Mindaugas Truncaitė, Lidija |
author_sort | Šimoliūnas, Eugenijus |
collection | PubMed |
description | A novel low-temperature siphovirus, vB_PagS_Vid5 (Vid5), was isolated in Lithuania using Pantoea agglomerans isolate for the phage propagation. The 61,437 bp genome of Vid5 has a G–C content of 48.8% and contains 99 probable protein encoding genes and one gene for tRNA(Ser). A comparative sequence analysis revealed that 46 out of 99 Vid5 open reading frames (ORFs) code for unique proteins that have no reliable identity to database entries. In total, 33 Vid5 ORFs were given a putative functional annotation, including those coding for the proteins responsible for virion morphogenesis, phage-host interactions, and DNA metabolism. In addition, a cluster of genes possibly involved in the biosynthesis of 7-deazaguanine derivatives was identified. Notably, one of these genes encodes a putative preQ(0)/preQ(1) transporter, which has never been detected in bacteriophages to date. A proteomic analysis led to the experimental identification of 11 virion proteins, including nine that were predicted by bioinformatics approaches. Based on the phylogenetic analysis, Vid5 cannot be assigned to any genus currently recognized by ICTV, and may represent a new one within the family of Siphoviridae. |
format | Online Article Text |
id | pubmed-6266253 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62662532018-12-07 Pantoea Bacteriophage vB_PagS_Vid5: A Low-Temperature Siphovirus That Harbors a Cluster of Genes Involved in the Biosynthesis of Archaeosine Šimoliūnas, Eugenijus Šimoliūnienė, Monika Kaliniene, Laura Zajančkauskaitė, Aurelija Skapas, Martynas Meškys, Rolandas Kaupinis, Algirdas Valius, Mindaugas Truncaitė, Lidija Viruses Article A novel low-temperature siphovirus, vB_PagS_Vid5 (Vid5), was isolated in Lithuania using Pantoea agglomerans isolate for the phage propagation. The 61,437 bp genome of Vid5 has a G–C content of 48.8% and contains 99 probable protein encoding genes and one gene for tRNA(Ser). A comparative sequence analysis revealed that 46 out of 99 Vid5 open reading frames (ORFs) code for unique proteins that have no reliable identity to database entries. In total, 33 Vid5 ORFs were given a putative functional annotation, including those coding for the proteins responsible for virion morphogenesis, phage-host interactions, and DNA metabolism. In addition, a cluster of genes possibly involved in the biosynthesis of 7-deazaguanine derivatives was identified. Notably, one of these genes encodes a putative preQ(0)/preQ(1) transporter, which has never been detected in bacteriophages to date. A proteomic analysis led to the experimental identification of 11 virion proteins, including nine that were predicted by bioinformatics approaches. Based on the phylogenetic analysis, Vid5 cannot be assigned to any genus currently recognized by ICTV, and may represent a new one within the family of Siphoviridae. MDPI 2018-10-25 /pmc/articles/PMC6266253/ /pubmed/30366363 http://dx.doi.org/10.3390/v10110583 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Šimoliūnas, Eugenijus Šimoliūnienė, Monika Kaliniene, Laura Zajančkauskaitė, Aurelija Skapas, Martynas Meškys, Rolandas Kaupinis, Algirdas Valius, Mindaugas Truncaitė, Lidija Pantoea Bacteriophage vB_PagS_Vid5: A Low-Temperature Siphovirus That Harbors a Cluster of Genes Involved in the Biosynthesis of Archaeosine |
title | Pantoea Bacteriophage vB_PagS_Vid5: A Low-Temperature Siphovirus That Harbors a Cluster of Genes Involved in the Biosynthesis of Archaeosine |
title_full | Pantoea Bacteriophage vB_PagS_Vid5: A Low-Temperature Siphovirus That Harbors a Cluster of Genes Involved in the Biosynthesis of Archaeosine |
title_fullStr | Pantoea Bacteriophage vB_PagS_Vid5: A Low-Temperature Siphovirus That Harbors a Cluster of Genes Involved in the Biosynthesis of Archaeosine |
title_full_unstemmed | Pantoea Bacteriophage vB_PagS_Vid5: A Low-Temperature Siphovirus That Harbors a Cluster of Genes Involved in the Biosynthesis of Archaeosine |
title_short | Pantoea Bacteriophage vB_PagS_Vid5: A Low-Temperature Siphovirus That Harbors a Cluster of Genes Involved in the Biosynthesis of Archaeosine |
title_sort | pantoea bacteriophage vb_pags_vid5: a low-temperature siphovirus that harbors a cluster of genes involved in the biosynthesis of archaeosine |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6266253/ https://www.ncbi.nlm.nih.gov/pubmed/30366363 http://dx.doi.org/10.3390/v10110583 |
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