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Pantoea Bacteriophage vB_PagS_Vid5: A Low-Temperature Siphovirus That Harbors a Cluster of Genes Involved in the Biosynthesis of Archaeosine

A novel low-temperature siphovirus, vB_PagS_Vid5 (Vid5), was isolated in Lithuania using Pantoea agglomerans isolate for the phage propagation. The 61,437 bp genome of Vid5 has a G–C content of 48.8% and contains 99 probable protein encoding genes and one gene for tRNA(Ser). A comparative sequence a...

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Autores principales: Šimoliūnas, Eugenijus, Šimoliūnienė, Monika, Kaliniene, Laura, Zajančkauskaitė, Aurelija, Skapas, Martynas, Meškys, Rolandas, Kaupinis, Algirdas, Valius, Mindaugas, Truncaitė, Lidija
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6266253/
https://www.ncbi.nlm.nih.gov/pubmed/30366363
http://dx.doi.org/10.3390/v10110583
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author Šimoliūnas, Eugenijus
Šimoliūnienė, Monika
Kaliniene, Laura
Zajančkauskaitė, Aurelija
Skapas, Martynas
Meškys, Rolandas
Kaupinis, Algirdas
Valius, Mindaugas
Truncaitė, Lidija
author_facet Šimoliūnas, Eugenijus
Šimoliūnienė, Monika
Kaliniene, Laura
Zajančkauskaitė, Aurelija
Skapas, Martynas
Meškys, Rolandas
Kaupinis, Algirdas
Valius, Mindaugas
Truncaitė, Lidija
author_sort Šimoliūnas, Eugenijus
collection PubMed
description A novel low-temperature siphovirus, vB_PagS_Vid5 (Vid5), was isolated in Lithuania using Pantoea agglomerans isolate for the phage propagation. The 61,437 bp genome of Vid5 has a G–C content of 48.8% and contains 99 probable protein encoding genes and one gene for tRNA(Ser). A comparative sequence analysis revealed that 46 out of 99 Vid5 open reading frames (ORFs) code for unique proteins that have no reliable identity to database entries. In total, 33 Vid5 ORFs were given a putative functional annotation, including those coding for the proteins responsible for virion morphogenesis, phage-host interactions, and DNA metabolism. In addition, a cluster of genes possibly involved in the biosynthesis of 7-deazaguanine derivatives was identified. Notably, one of these genes encodes a putative preQ(0)/preQ(1) transporter, which has never been detected in bacteriophages to date. A proteomic analysis led to the experimental identification of 11 virion proteins, including nine that were predicted by bioinformatics approaches. Based on the phylogenetic analysis, Vid5 cannot be assigned to any genus currently recognized by ICTV, and may represent a new one within the family of Siphoviridae.
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spelling pubmed-62662532018-12-07 Pantoea Bacteriophage vB_PagS_Vid5: A Low-Temperature Siphovirus That Harbors a Cluster of Genes Involved in the Biosynthesis of Archaeosine Šimoliūnas, Eugenijus Šimoliūnienė, Monika Kaliniene, Laura Zajančkauskaitė, Aurelija Skapas, Martynas Meškys, Rolandas Kaupinis, Algirdas Valius, Mindaugas Truncaitė, Lidija Viruses Article A novel low-temperature siphovirus, vB_PagS_Vid5 (Vid5), was isolated in Lithuania using Pantoea agglomerans isolate for the phage propagation. The 61,437 bp genome of Vid5 has a G–C content of 48.8% and contains 99 probable protein encoding genes and one gene for tRNA(Ser). A comparative sequence analysis revealed that 46 out of 99 Vid5 open reading frames (ORFs) code for unique proteins that have no reliable identity to database entries. In total, 33 Vid5 ORFs were given a putative functional annotation, including those coding for the proteins responsible for virion morphogenesis, phage-host interactions, and DNA metabolism. In addition, a cluster of genes possibly involved in the biosynthesis of 7-deazaguanine derivatives was identified. Notably, one of these genes encodes a putative preQ(0)/preQ(1) transporter, which has never been detected in bacteriophages to date. A proteomic analysis led to the experimental identification of 11 virion proteins, including nine that were predicted by bioinformatics approaches. Based on the phylogenetic analysis, Vid5 cannot be assigned to any genus currently recognized by ICTV, and may represent a new one within the family of Siphoviridae. MDPI 2018-10-25 /pmc/articles/PMC6266253/ /pubmed/30366363 http://dx.doi.org/10.3390/v10110583 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Šimoliūnas, Eugenijus
Šimoliūnienė, Monika
Kaliniene, Laura
Zajančkauskaitė, Aurelija
Skapas, Martynas
Meškys, Rolandas
Kaupinis, Algirdas
Valius, Mindaugas
Truncaitė, Lidija
Pantoea Bacteriophage vB_PagS_Vid5: A Low-Temperature Siphovirus That Harbors a Cluster of Genes Involved in the Biosynthesis of Archaeosine
title Pantoea Bacteriophage vB_PagS_Vid5: A Low-Temperature Siphovirus That Harbors a Cluster of Genes Involved in the Biosynthesis of Archaeosine
title_full Pantoea Bacteriophage vB_PagS_Vid5: A Low-Temperature Siphovirus That Harbors a Cluster of Genes Involved in the Biosynthesis of Archaeosine
title_fullStr Pantoea Bacteriophage vB_PagS_Vid5: A Low-Temperature Siphovirus That Harbors a Cluster of Genes Involved in the Biosynthesis of Archaeosine
title_full_unstemmed Pantoea Bacteriophage vB_PagS_Vid5: A Low-Temperature Siphovirus That Harbors a Cluster of Genes Involved in the Biosynthesis of Archaeosine
title_short Pantoea Bacteriophage vB_PagS_Vid5: A Low-Temperature Siphovirus That Harbors a Cluster of Genes Involved in the Biosynthesis of Archaeosine
title_sort pantoea bacteriophage vb_pags_vid5: a low-temperature siphovirus that harbors a cluster of genes involved in the biosynthesis of archaeosine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6266253/
https://www.ncbi.nlm.nih.gov/pubmed/30366363
http://dx.doi.org/10.3390/v10110583
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