Cargando…

β-Cyclodextrin Does not Alter the Bioaccessibility and the Uptake by Caco-2 Cells of Olive By-Product Phenolic Compounds

Alperujo—a two-phase olive mill waste that is composed of olive vegetation water and solid skin, pulp, and seed fragments - is a highly valuable olive by-product due to its high content in phenolic compounds. In this study, we assessed whether β-cyclodextrin (β-CD), which is used to extract and prot...

Descripción completa

Detalles Bibliográficos
Autores principales: Malapert, Aurélia, Tomao, Valérie, Margier, Marielle, Nowicki, Marion, Gleize, Béatrice, Dangles, Olivier, Reboul, Emmanuelle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6266305/
https://www.ncbi.nlm.nih.gov/pubmed/30400310
http://dx.doi.org/10.3390/nu10111653
_version_ 1783375808800227328
author Malapert, Aurélia
Tomao, Valérie
Margier, Marielle
Nowicki, Marion
Gleize, Béatrice
Dangles, Olivier
Reboul, Emmanuelle
author_facet Malapert, Aurélia
Tomao, Valérie
Margier, Marielle
Nowicki, Marion
Gleize, Béatrice
Dangles, Olivier
Reboul, Emmanuelle
author_sort Malapert, Aurélia
collection PubMed
description Alperujo—a two-phase olive mill waste that is composed of olive vegetation water and solid skin, pulp, and seed fragments - is a highly valuable olive by-product due to its high content in phenolic compounds. In this study, we assessed whether β-cyclodextrin (β-CD), which is used to extract and protect alpejuro phenolic compounds (hydroxytyrosol-O-glucoside, tyrosol, caffeic, and p-coumaric acids) could impact on their bioaccessibility (i.e., the percentage of molecule found in the aqueous phase of the digesta) and uptake by intestinal cells, by using an in vitro digestion model and Caco-2 TC7 cells in culture, respectively. Our results showed that β-CD did not change the bioaccessibility of the selected phenols. Hydroxytyrosol-O-glucoside and caffeic did not cross Caco-2 cell monolayers. Conversely ferulic acid, identified as the main caffeic acid intestinal metabolite, was absorbed through intestinal cell monolayers (~20%). Interestingly, β-CD moderately but significantly improved the local absorption of tyrosol and p-coumaric acid (2.3 + 1.4% and 8.5 ± 4.2%, respectively, p < 0.05), even if their final bioavailability (expressed as bioaccessibility × absorption by Caco-2 cells) was not modified (16.2 ± 0.6% vs. 16.8 ± 0.5% for tyrosol and 32.0 ± 3.2% vs. 37.2 ± 3.2% for p-coumaric acid, from pure alperujo and alperujo complexed with β-CD, respectively). Overall, our results show that β-CD is an interesting extraction and storage agent for phenolic compounds that does not alter their in vitro bioavailability.
format Online
Article
Text
id pubmed-6266305
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-62663052018-12-06 β-Cyclodextrin Does not Alter the Bioaccessibility and the Uptake by Caco-2 Cells of Olive By-Product Phenolic Compounds Malapert, Aurélia Tomao, Valérie Margier, Marielle Nowicki, Marion Gleize, Béatrice Dangles, Olivier Reboul, Emmanuelle Nutrients Article Alperujo—a two-phase olive mill waste that is composed of olive vegetation water and solid skin, pulp, and seed fragments - is a highly valuable olive by-product due to its high content in phenolic compounds. In this study, we assessed whether β-cyclodextrin (β-CD), which is used to extract and protect alpejuro phenolic compounds (hydroxytyrosol-O-glucoside, tyrosol, caffeic, and p-coumaric acids) could impact on their bioaccessibility (i.e., the percentage of molecule found in the aqueous phase of the digesta) and uptake by intestinal cells, by using an in vitro digestion model and Caco-2 TC7 cells in culture, respectively. Our results showed that β-CD did not change the bioaccessibility of the selected phenols. Hydroxytyrosol-O-glucoside and caffeic did not cross Caco-2 cell monolayers. Conversely ferulic acid, identified as the main caffeic acid intestinal metabolite, was absorbed through intestinal cell monolayers (~20%). Interestingly, β-CD moderately but significantly improved the local absorption of tyrosol and p-coumaric acid (2.3 + 1.4% and 8.5 ± 4.2%, respectively, p < 0.05), even if their final bioavailability (expressed as bioaccessibility × absorption by Caco-2 cells) was not modified (16.2 ± 0.6% vs. 16.8 ± 0.5% for tyrosol and 32.0 ± 3.2% vs. 37.2 ± 3.2% for p-coumaric acid, from pure alperujo and alperujo complexed with β-CD, respectively). Overall, our results show that β-CD is an interesting extraction and storage agent for phenolic compounds that does not alter their in vitro bioavailability. MDPI 2018-11-03 /pmc/articles/PMC6266305/ /pubmed/30400310 http://dx.doi.org/10.3390/nu10111653 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Malapert, Aurélia
Tomao, Valérie
Margier, Marielle
Nowicki, Marion
Gleize, Béatrice
Dangles, Olivier
Reboul, Emmanuelle
β-Cyclodextrin Does not Alter the Bioaccessibility and the Uptake by Caco-2 Cells of Olive By-Product Phenolic Compounds
title β-Cyclodextrin Does not Alter the Bioaccessibility and the Uptake by Caco-2 Cells of Olive By-Product Phenolic Compounds
title_full β-Cyclodextrin Does not Alter the Bioaccessibility and the Uptake by Caco-2 Cells of Olive By-Product Phenolic Compounds
title_fullStr β-Cyclodextrin Does not Alter the Bioaccessibility and the Uptake by Caco-2 Cells of Olive By-Product Phenolic Compounds
title_full_unstemmed β-Cyclodextrin Does not Alter the Bioaccessibility and the Uptake by Caco-2 Cells of Olive By-Product Phenolic Compounds
title_short β-Cyclodextrin Does not Alter the Bioaccessibility and the Uptake by Caco-2 Cells of Olive By-Product Phenolic Compounds
title_sort β-cyclodextrin does not alter the bioaccessibility and the uptake by caco-2 cells of olive by-product phenolic compounds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6266305/
https://www.ncbi.nlm.nih.gov/pubmed/30400310
http://dx.doi.org/10.3390/nu10111653
work_keys_str_mv AT malapertaurelia bcyclodextrindoesnotalterthebioaccessibilityandtheuptakebycaco2cellsofolivebyproductphenoliccompounds
AT tomaovalerie bcyclodextrindoesnotalterthebioaccessibilityandtheuptakebycaco2cellsofolivebyproductphenoliccompounds
AT margiermarielle bcyclodextrindoesnotalterthebioaccessibilityandtheuptakebycaco2cellsofolivebyproductphenoliccompounds
AT nowickimarion bcyclodextrindoesnotalterthebioaccessibilityandtheuptakebycaco2cellsofolivebyproductphenoliccompounds
AT gleizebeatrice bcyclodextrindoesnotalterthebioaccessibilityandtheuptakebycaco2cellsofolivebyproductphenoliccompounds
AT danglesolivier bcyclodextrindoesnotalterthebioaccessibilityandtheuptakebycaco2cellsofolivebyproductphenoliccompounds
AT reboulemmanuelle bcyclodextrindoesnotalterthebioaccessibilityandtheuptakebycaco2cellsofolivebyproductphenoliccompounds