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POFUT1 as a Promising Novel Biomarker of Colorectal Cancer

Background: While protein O-fucosyltransferase 1 (POFUT1) overexpression has been recently proposed as a potential biomarker for different cancer types, no study was carried out on POFUT1 implication in colorectal cancer (CRC). Methods: Data from 626 tumors and 51 non-tumor adjacent tissues availabl...

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Autores principales: Chabanais, Julien, Labrousse, François, Chaunavel, Alain, Germot, Agnès, Maftah, Abderrahman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6266312/
https://www.ncbi.nlm.nih.gov/pubmed/30380753
http://dx.doi.org/10.3390/cancers10110411
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author Chabanais, Julien
Labrousse, François
Chaunavel, Alain
Germot, Agnès
Maftah, Abderrahman
author_facet Chabanais, Julien
Labrousse, François
Chaunavel, Alain
Germot, Agnès
Maftah, Abderrahman
author_sort Chabanais, Julien
collection PubMed
description Background: While protein O-fucosyltransferase 1 (POFUT1) overexpression has been recently proposed as a potential biomarker for different cancer types, no study was carried out on POFUT1 implication in colorectal cancer (CRC). Methods: Data from 626 tumors and 51 non-tumor adjacent tissues available in FireBrowse had been used in this study. Statistical analyses on POFUT1 expression and gene copy number, NOTCH receptors (main targets of POFUT1 enzymatic activity) expression and association of POFUT1 and NOTCH1 expressions with clinical parameters were investigated. Data were completed by POFUT1 histological labeling on six tumor tissues from patients with CRC. Results: We found that POFUT1 is overexpressed from the stage I (p < 0.001) and 76.02% of tumors have a 20q11.21 amplification, associated in 90.13% of cases with a POFUT1 overexpression, compared to non-tumor adjacent tissues. The POFUT1 copy number in tumors is mainly between 2 and 3. POFUT1 is positively correlated with NOTCH1 (r(s) = 0.34, p < 0.001), NOTCH3 (r(s) = 0.087, p = 0.0297), and NOTCH4 (r(s) = 0.097, p = 0.0148) expressions, while negatively correlated with NOTCH2 expression (r(s) = −0.098, p = 0.0142). POFUT1 overexpression is markedly associated with rectal location, non-mucinous adenocarcinoma and cancer stages IV and M1. NOTCH1 overexpression is only associated with rectal location and non-mucinous adenocarcinoma. Conclusion: We conclude that POFUT1 is overexpressed in CRC from stage I, and its high expression is associated with metastatic process, probably through NOTCH pathway activation. Then, POFUT1 could represent a potential novel biomarker for CRC diagnosis.
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spelling pubmed-62663122018-12-03 POFUT1 as a Promising Novel Biomarker of Colorectal Cancer Chabanais, Julien Labrousse, François Chaunavel, Alain Germot, Agnès Maftah, Abderrahman Cancers (Basel) Article Background: While protein O-fucosyltransferase 1 (POFUT1) overexpression has been recently proposed as a potential biomarker for different cancer types, no study was carried out on POFUT1 implication in colorectal cancer (CRC). Methods: Data from 626 tumors and 51 non-tumor adjacent tissues available in FireBrowse had been used in this study. Statistical analyses on POFUT1 expression and gene copy number, NOTCH receptors (main targets of POFUT1 enzymatic activity) expression and association of POFUT1 and NOTCH1 expressions with clinical parameters were investigated. Data were completed by POFUT1 histological labeling on six tumor tissues from patients with CRC. Results: We found that POFUT1 is overexpressed from the stage I (p < 0.001) and 76.02% of tumors have a 20q11.21 amplification, associated in 90.13% of cases with a POFUT1 overexpression, compared to non-tumor adjacent tissues. The POFUT1 copy number in tumors is mainly between 2 and 3. POFUT1 is positively correlated with NOTCH1 (r(s) = 0.34, p < 0.001), NOTCH3 (r(s) = 0.087, p = 0.0297), and NOTCH4 (r(s) = 0.097, p = 0.0148) expressions, while negatively correlated with NOTCH2 expression (r(s) = −0.098, p = 0.0142). POFUT1 overexpression is markedly associated with rectal location, non-mucinous adenocarcinoma and cancer stages IV and M1. NOTCH1 overexpression is only associated with rectal location and non-mucinous adenocarcinoma. Conclusion: We conclude that POFUT1 is overexpressed in CRC from stage I, and its high expression is associated with metastatic process, probably through NOTCH pathway activation. Then, POFUT1 could represent a potential novel biomarker for CRC diagnosis. MDPI 2018-10-30 /pmc/articles/PMC6266312/ /pubmed/30380753 http://dx.doi.org/10.3390/cancers10110411 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chabanais, Julien
Labrousse, François
Chaunavel, Alain
Germot, Agnès
Maftah, Abderrahman
POFUT1 as a Promising Novel Biomarker of Colorectal Cancer
title POFUT1 as a Promising Novel Biomarker of Colorectal Cancer
title_full POFUT1 as a Promising Novel Biomarker of Colorectal Cancer
title_fullStr POFUT1 as a Promising Novel Biomarker of Colorectal Cancer
title_full_unstemmed POFUT1 as a Promising Novel Biomarker of Colorectal Cancer
title_short POFUT1 as a Promising Novel Biomarker of Colorectal Cancer
title_sort pofut1 as a promising novel biomarker of colorectal cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6266312/
https://www.ncbi.nlm.nih.gov/pubmed/30380753
http://dx.doi.org/10.3390/cancers10110411
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