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Cell Injection Millirobot Development and Evaluation in Microfluidic Chip

We propose an innovative design of millirobot, which can achieve donor cell suction, delivery, and injection in a mammalian oocyte on a microfluidic chip. The millirobot body contains a hollow space that produces suction and ejection forces for the injection of cell nuclei using a nozzle at the tip...

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Detalles Bibliográficos
Autores principales: Feng, Lin, Zhou, Qiang, Song, Bin, Feng, Yanmin, Cai, Jun, Jiang, Yonggang, Zhang, Deyuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6266326/
https://www.ncbi.nlm.nih.gov/pubmed/30428554
http://dx.doi.org/10.3390/mi9110590
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author Feng, Lin
Zhou, Qiang
Song, Bin
Feng, Yanmin
Cai, Jun
Jiang, Yonggang
Zhang, Deyuan
author_facet Feng, Lin
Zhou, Qiang
Song, Bin
Feng, Yanmin
Cai, Jun
Jiang, Yonggang
Zhang, Deyuan
author_sort Feng, Lin
collection PubMed
description We propose an innovative design of millirobot, which can achieve donor cell suction, delivery, and injection in a mammalian oocyte on a microfluidic chip. The millirobot body contains a hollow space that produces suction and ejection forces for the injection of cell nuclei using a nozzle at the tip of the robot. Specifically, a controller changes the hollow volume by balancing the magnetic and elastic forces of a membrane along with the motion of stages in the XY plane. A glass capillary attached to the tip of the robot contains a nozzle that is able to absorb and inject cell nuclei. The millirobot provides three degrees of freedom and generates micronewton forces. We demonstrate the effectiveness of the proposed millirobot through an experiment of the absorption and ejection of 20-µm particles from the nozzle using magnetic control in a microfluidic chip.
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spelling pubmed-62663262018-12-06 Cell Injection Millirobot Development and Evaluation in Microfluidic Chip Feng, Lin Zhou, Qiang Song, Bin Feng, Yanmin Cai, Jun Jiang, Yonggang Zhang, Deyuan Micromachines (Basel) Article We propose an innovative design of millirobot, which can achieve donor cell suction, delivery, and injection in a mammalian oocyte on a microfluidic chip. The millirobot body contains a hollow space that produces suction and ejection forces for the injection of cell nuclei using a nozzle at the tip of the robot. Specifically, a controller changes the hollow volume by balancing the magnetic and elastic forces of a membrane along with the motion of stages in the XY plane. A glass capillary attached to the tip of the robot contains a nozzle that is able to absorb and inject cell nuclei. The millirobot provides three degrees of freedom and generates micronewton forces. We demonstrate the effectiveness of the proposed millirobot through an experiment of the absorption and ejection of 20-µm particles from the nozzle using magnetic control in a microfluidic chip. MDPI 2018-11-13 /pmc/articles/PMC6266326/ /pubmed/30428554 http://dx.doi.org/10.3390/mi9110590 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Feng, Lin
Zhou, Qiang
Song, Bin
Feng, Yanmin
Cai, Jun
Jiang, Yonggang
Zhang, Deyuan
Cell Injection Millirobot Development and Evaluation in Microfluidic Chip
title Cell Injection Millirobot Development and Evaluation in Microfluidic Chip
title_full Cell Injection Millirobot Development and Evaluation in Microfluidic Chip
title_fullStr Cell Injection Millirobot Development and Evaluation in Microfluidic Chip
title_full_unstemmed Cell Injection Millirobot Development and Evaluation in Microfluidic Chip
title_short Cell Injection Millirobot Development and Evaluation in Microfluidic Chip
title_sort cell injection millirobot development and evaluation in microfluidic chip
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6266326/
https://www.ncbi.nlm.nih.gov/pubmed/30428554
http://dx.doi.org/10.3390/mi9110590
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