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Development of a novel in situ gelling skin dressing: Delivering high levels of dissolved oxygen at pH 5.5.

BACKGROUND AND AIMS: Wound healing requires appropriate oxygen and pH levels. Oxygen therapy and pH‐modulating treatments have shown positive effects on wound healing. Thus, a dressing, which combines high levels of dissolved oxygen (DO) with the pH of intact skin, may improve wound healing. Our aim...

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Autores principales: Moen, Ingrid, Ugland, Hege, Strömberg, Niklas, Sjöström, Eva, Karlson, Anders, Ringstad, Lovisa, Bysell, Helena, Amiry‐Moghaddam, Mahmood, Haglerød, Camilla
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6266454/
https://www.ncbi.nlm.nih.gov/pubmed/30623087
http://dx.doi.org/10.1002/hsr2.57
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author Moen, Ingrid
Ugland, Hege
Strömberg, Niklas
Sjöström, Eva
Karlson, Anders
Ringstad, Lovisa
Bysell, Helena
Amiry‐Moghaddam, Mahmood
Haglerød, Camilla
author_facet Moen, Ingrid
Ugland, Hege
Strömberg, Niklas
Sjöström, Eva
Karlson, Anders
Ringstad, Lovisa
Bysell, Helena
Amiry‐Moghaddam, Mahmood
Haglerød, Camilla
author_sort Moen, Ingrid
collection PubMed
description BACKGROUND AND AIMS: Wound healing requires appropriate oxygen and pH levels. Oxygen therapy and pH‐modulating treatments have shown positive effects on wound healing. Thus, a dressing, which combines high levels of dissolved oxygen (DO) with the pH of intact skin, may improve wound healing. Our aims were to (1) formulate an in situ gelling dressing with high levels of DO and with the pH level of intact skin, (2) evaluate physical and chemical properties of the dressing, and (3) elucidate basic effects of elevated levels of DO on human skin cells in vitro. METHODS: A dressing was formulated with 15 to 16 wt% poloxamer 407, acetate buffer, and oxygenated water. Stability of pH and DO, rheology, and shelf life were analysed. Furthermore, in vitro studies of the effect of increased levels of DO were performed. RESULTS: An in situ gelling wound dressing, with a DO concentration ranging between 25 and 35 mg/L and a pH of 5.5, was formulated. The DO concentration was stable above 22 mg/L for at least 30 hours when applied on a surface at 35°C and covered for directed diffusion into the intended wound area. At storage, the dressing had stable pH for 3 months and stable DO concentration over 30 mg/L for 7 weeks. Increasing DO significantly enhanced intracellular ATP in human skin cells, without changing reactive oxygen species production, proliferation rate, or viability. CONCLUSION: The developed dressing may facilitate wound healing by delivering controlled and stable oxygen levels, providing adjustable pH for optimized healing, and increasing intracellular ATP availability.
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spelling pubmed-62664542019-01-08 Development of a novel in situ gelling skin dressing: Delivering high levels of dissolved oxygen at pH 5.5. Moen, Ingrid Ugland, Hege Strömberg, Niklas Sjöström, Eva Karlson, Anders Ringstad, Lovisa Bysell, Helena Amiry‐Moghaddam, Mahmood Haglerød, Camilla Health Sci Rep Research Articles BACKGROUND AND AIMS: Wound healing requires appropriate oxygen and pH levels. Oxygen therapy and pH‐modulating treatments have shown positive effects on wound healing. Thus, a dressing, which combines high levels of dissolved oxygen (DO) with the pH of intact skin, may improve wound healing. Our aims were to (1) formulate an in situ gelling dressing with high levels of DO and with the pH level of intact skin, (2) evaluate physical and chemical properties of the dressing, and (3) elucidate basic effects of elevated levels of DO on human skin cells in vitro. METHODS: A dressing was formulated with 15 to 16 wt% poloxamer 407, acetate buffer, and oxygenated water. Stability of pH and DO, rheology, and shelf life were analysed. Furthermore, in vitro studies of the effect of increased levels of DO were performed. RESULTS: An in situ gelling wound dressing, with a DO concentration ranging between 25 and 35 mg/L and a pH of 5.5, was formulated. The DO concentration was stable above 22 mg/L for at least 30 hours when applied on a surface at 35°C and covered for directed diffusion into the intended wound area. At storage, the dressing had stable pH for 3 months and stable DO concentration over 30 mg/L for 7 weeks. Increasing DO significantly enhanced intracellular ATP in human skin cells, without changing reactive oxygen species production, proliferation rate, or viability. CONCLUSION: The developed dressing may facilitate wound healing by delivering controlled and stable oxygen levels, providing adjustable pH for optimized healing, and increasing intracellular ATP availability. John Wiley and Sons Inc. 2018-06-13 /pmc/articles/PMC6266454/ /pubmed/30623087 http://dx.doi.org/10.1002/hsr2.57 Text en © 2018 The Authors. Health Science Reports published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Research Articles
Moen, Ingrid
Ugland, Hege
Strömberg, Niklas
Sjöström, Eva
Karlson, Anders
Ringstad, Lovisa
Bysell, Helena
Amiry‐Moghaddam, Mahmood
Haglerød, Camilla
Development of a novel in situ gelling skin dressing: Delivering high levels of dissolved oxygen at pH 5.5.
title Development of a novel in situ gelling skin dressing: Delivering high levels of dissolved oxygen at pH 5.5.
title_full Development of a novel in situ gelling skin dressing: Delivering high levels of dissolved oxygen at pH 5.5.
title_fullStr Development of a novel in situ gelling skin dressing: Delivering high levels of dissolved oxygen at pH 5.5.
title_full_unstemmed Development of a novel in situ gelling skin dressing: Delivering high levels of dissolved oxygen at pH 5.5.
title_short Development of a novel in situ gelling skin dressing: Delivering high levels of dissolved oxygen at pH 5.5.
title_sort development of a novel in situ gelling skin dressing: delivering high levels of dissolved oxygen at ph 5.5.
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6266454/
https://www.ncbi.nlm.nih.gov/pubmed/30623087
http://dx.doi.org/10.1002/hsr2.57
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