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Blackcurrant Supplementation Improves Trabecular Bone Mass in Young but Not Aged Mice

Due to deleterious side effects of currently available medications, the search for novel, safe, and effective preventive agents for improving bone health in aging continues and is urgently needed. This study aimed to determine whether dietary blackcurrants (BC), an anthocyanin-rich berry, can improv...

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Autores principales: Sakaki, Junichi, Melough, Melissa, Lee, Sang Gil, Kalinowski, Judy, Koo, Sung I., Lee, Sun-Kyeong, Chun, Ock K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6266496/
https://www.ncbi.nlm.nih.gov/pubmed/30400569
http://dx.doi.org/10.3390/nu10111671
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author Sakaki, Junichi
Melough, Melissa
Lee, Sang Gil
Kalinowski, Judy
Koo, Sung I.
Lee, Sun-Kyeong
Chun, Ock K.
author_facet Sakaki, Junichi
Melough, Melissa
Lee, Sang Gil
Kalinowski, Judy
Koo, Sung I.
Lee, Sun-Kyeong
Chun, Ock K.
author_sort Sakaki, Junichi
collection PubMed
description Due to deleterious side effects of currently available medications, the search for novel, safe, and effective preventive agents for improving bone health in aging continues and is urgently needed. This study aimed to determine whether dietary blackcurrants (BC), an anthocyanin-rich berry, can improve bone mass in a mouse model of age-related bone loss. Thirty-five female C57BL/6J mice, 3 months old (n = 20) and 18 months old (n = 15), were randomized to consume either a standard chow diet or a standard chow diet with 1% (w/w) BC for four months. Dual-energy X-ray absorptiometry, Micro computed tomography (µCT), and histomorphometric analyses were conducted to assess bone parameters on femurs. Biochemical assays were conducted to determine bone resorption, antioxidant activity, and inflammation in humerus homogenates. Trabecular bone volume (BV/TV) was significantly lower in aged mice compared to young mice (young control, 3.7 ± 0.4% vs aged control, 1.5 ± 0.5%, mean ± SEM (standard error of mean), p < 0.01; young BC, 5.3 ± 0.6% vs aged BC, 1.1 ± 0.3%, p < 0.001). µCT analysis revealed that BC supplementation increased trabecular BV/TV in young mice by 43.2% (p < 0.05) compared to controls. Histomorphometric analysis revealed a 50% increase, though this effect was not statistically significant (p = 0.07). The osteoblast surface increased by 82.5% in aged mice with BC compared to controls (p < 0.01). In humerus homogenates of young mice, BC consumption reduced C-telopeptide of type I collagen by 12.4% (p < 0.05) and increased glutathione peroxidase by 96.4% (p < 0.05). In humerus homogenates of aged mice, BC consumption increased catalase by 12% (p = 0.09). Aged mice had significantly elevated concentrations of tumor necrosis factor α (TNF-α), a pro-inflammatory cytokine contributing to bone resorption, which was reduced by 43.3% with BC consumption (p = 0.06). These results suggest that early consumption of BC may protect from aging-associated bone loss.
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spelling pubmed-62664962018-12-06 Blackcurrant Supplementation Improves Trabecular Bone Mass in Young but Not Aged Mice Sakaki, Junichi Melough, Melissa Lee, Sang Gil Kalinowski, Judy Koo, Sung I. Lee, Sun-Kyeong Chun, Ock K. Nutrients Article Due to deleterious side effects of currently available medications, the search for novel, safe, and effective preventive agents for improving bone health in aging continues and is urgently needed. This study aimed to determine whether dietary blackcurrants (BC), an anthocyanin-rich berry, can improve bone mass in a mouse model of age-related bone loss. Thirty-five female C57BL/6J mice, 3 months old (n = 20) and 18 months old (n = 15), were randomized to consume either a standard chow diet or a standard chow diet with 1% (w/w) BC for four months. Dual-energy X-ray absorptiometry, Micro computed tomography (µCT), and histomorphometric analyses were conducted to assess bone parameters on femurs. Biochemical assays were conducted to determine bone resorption, antioxidant activity, and inflammation in humerus homogenates. Trabecular bone volume (BV/TV) was significantly lower in aged mice compared to young mice (young control, 3.7 ± 0.4% vs aged control, 1.5 ± 0.5%, mean ± SEM (standard error of mean), p < 0.01; young BC, 5.3 ± 0.6% vs aged BC, 1.1 ± 0.3%, p < 0.001). µCT analysis revealed that BC supplementation increased trabecular BV/TV in young mice by 43.2% (p < 0.05) compared to controls. Histomorphometric analysis revealed a 50% increase, though this effect was not statistically significant (p = 0.07). The osteoblast surface increased by 82.5% in aged mice with BC compared to controls (p < 0.01). In humerus homogenates of young mice, BC consumption reduced C-telopeptide of type I collagen by 12.4% (p < 0.05) and increased glutathione peroxidase by 96.4% (p < 0.05). In humerus homogenates of aged mice, BC consumption increased catalase by 12% (p = 0.09). Aged mice had significantly elevated concentrations of tumor necrosis factor α (TNF-α), a pro-inflammatory cytokine contributing to bone resorption, which was reduced by 43.3% with BC consumption (p = 0.06). These results suggest that early consumption of BC may protect from aging-associated bone loss. MDPI 2018-11-05 /pmc/articles/PMC6266496/ /pubmed/30400569 http://dx.doi.org/10.3390/nu10111671 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sakaki, Junichi
Melough, Melissa
Lee, Sang Gil
Kalinowski, Judy
Koo, Sung I.
Lee, Sun-Kyeong
Chun, Ock K.
Blackcurrant Supplementation Improves Trabecular Bone Mass in Young but Not Aged Mice
title Blackcurrant Supplementation Improves Trabecular Bone Mass in Young but Not Aged Mice
title_full Blackcurrant Supplementation Improves Trabecular Bone Mass in Young but Not Aged Mice
title_fullStr Blackcurrant Supplementation Improves Trabecular Bone Mass in Young but Not Aged Mice
title_full_unstemmed Blackcurrant Supplementation Improves Trabecular Bone Mass in Young but Not Aged Mice
title_short Blackcurrant Supplementation Improves Trabecular Bone Mass in Young but Not Aged Mice
title_sort blackcurrant supplementation improves trabecular bone mass in young but not aged mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6266496/
https://www.ncbi.nlm.nih.gov/pubmed/30400569
http://dx.doi.org/10.3390/nu10111671
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