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Trimetazidine Protects Against Atherosclerosis by Changing Energy Charge and Oxidative Stress
BACKGROUND: This study investigated the effect and the possible mechanism of trimetazidine in atherosclerosis. MATERIAL/METHODS: We established an atherosclerotic rat model by high-fat diet and vitamin D injection. Rats were separated into 3 different groups: control, atherosclerosis, and trimetazid...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Scientific Literature, Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6266541/ https://www.ncbi.nlm.nih.gov/pubmed/30468686 http://dx.doi.org/10.12659/MSM.911317 |
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author | Zheng, Shuzhan Du, Yanfei Peng, Qiqi Fan, Xinrong Li, Jiafu Chen, Mao |
author_facet | Zheng, Shuzhan Du, Yanfei Peng, Qiqi Fan, Xinrong Li, Jiafu Chen, Mao |
author_sort | Zheng, Shuzhan |
collection | PubMed |
description | BACKGROUND: This study investigated the effect and the possible mechanism of trimetazidine in atherosclerosis. MATERIAL/METHODS: We established an atherosclerotic rat model by high-fat diet and vitamin D injection. Rats were separated into 3 different groups: control, atherosclerosis, and trimetazidine (n=10). The aortic artery was isolated and its morphological features were examined by hematoxylin and eosin (HE) staining. Serum low-density lipoprotein cholesterol (LDL-c), total cholesterol (TC), and triglycerides (TG) were analyzed using an automatic biochemical analyzer. Human aortic smooth muscle cells (HASMCs) were cultured and divided into 5 groups: no treatment, H(2)O(2) treatment only, trimetazidine preincubation before H(2)O(2) treatment, oxidized low-density lipoprotein (oxLDL) treatment only, and trimetazidine preincubation before oxLDL treatment. HASMCs proliferation was tested using the Cell Counting Kit-8. Reactive oxygen species (ROS) and malondialdehyde (MDA) levels, superoxide dismutase (SOD) activity of the aortic artery, and HASMCs were measured using commercially available kits. RESULTS: HE staining assay showed that trimetazidine suppressed the progression of atherosclerosis and reduced foam cell formation in the aortic artery without affecting serum lipid levels. HASMCs proliferation assay revealed that trimetazidine alleviated the inhibitory effect of H(2)O(2) on HASMCs proliferation and inhibited oxLDL-induced proliferation of HASMCs. Moreover, trimetazidine ameliorated ROS up-regulation elicited by H(2)O(2) or oxLDL in HASMCs. Additionally, trimetazidine restored SOD activity and reduced MDA content of HASMCs. CONCLUSIONS: Trimetazidine suppressed the progression of atherosclerosis by enhancing energy value, decreasing ROS level of aortic artery, modulating HASMCs proliferation, and reducing oxidative stress in HASMCs. |
format | Online Article Text |
id | pubmed-6266541 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | International Scientific Literature, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-62665412018-12-21 Trimetazidine Protects Against Atherosclerosis by Changing Energy Charge and Oxidative Stress Zheng, Shuzhan Du, Yanfei Peng, Qiqi Fan, Xinrong Li, Jiafu Chen, Mao Med Sci Monit Animal Study BACKGROUND: This study investigated the effect and the possible mechanism of trimetazidine in atherosclerosis. MATERIAL/METHODS: We established an atherosclerotic rat model by high-fat diet and vitamin D injection. Rats were separated into 3 different groups: control, atherosclerosis, and trimetazidine (n=10). The aortic artery was isolated and its morphological features were examined by hematoxylin and eosin (HE) staining. Serum low-density lipoprotein cholesterol (LDL-c), total cholesterol (TC), and triglycerides (TG) were analyzed using an automatic biochemical analyzer. Human aortic smooth muscle cells (HASMCs) were cultured and divided into 5 groups: no treatment, H(2)O(2) treatment only, trimetazidine preincubation before H(2)O(2) treatment, oxidized low-density lipoprotein (oxLDL) treatment only, and trimetazidine preincubation before oxLDL treatment. HASMCs proliferation was tested using the Cell Counting Kit-8. Reactive oxygen species (ROS) and malondialdehyde (MDA) levels, superoxide dismutase (SOD) activity of the aortic artery, and HASMCs were measured using commercially available kits. RESULTS: HE staining assay showed that trimetazidine suppressed the progression of atherosclerosis and reduced foam cell formation in the aortic artery without affecting serum lipid levels. HASMCs proliferation assay revealed that trimetazidine alleviated the inhibitory effect of H(2)O(2) on HASMCs proliferation and inhibited oxLDL-induced proliferation of HASMCs. Moreover, trimetazidine ameliorated ROS up-regulation elicited by H(2)O(2) or oxLDL in HASMCs. Additionally, trimetazidine restored SOD activity and reduced MDA content of HASMCs. CONCLUSIONS: Trimetazidine suppressed the progression of atherosclerosis by enhancing energy value, decreasing ROS level of aortic artery, modulating HASMCs proliferation, and reducing oxidative stress in HASMCs. International Scientific Literature, Inc. 2018-11-23 /pmc/articles/PMC6266541/ /pubmed/30468686 http://dx.doi.org/10.12659/MSM.911317 Text en © Med Sci Monit, 2018 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) ) |
spellingShingle | Animal Study Zheng, Shuzhan Du, Yanfei Peng, Qiqi Fan, Xinrong Li, Jiafu Chen, Mao Trimetazidine Protects Against Atherosclerosis by Changing Energy Charge and Oxidative Stress |
title | Trimetazidine Protects Against Atherosclerosis by Changing Energy Charge and Oxidative Stress |
title_full | Trimetazidine Protects Against Atherosclerosis by Changing Energy Charge and Oxidative Stress |
title_fullStr | Trimetazidine Protects Against Atherosclerosis by Changing Energy Charge and Oxidative Stress |
title_full_unstemmed | Trimetazidine Protects Against Atherosclerosis by Changing Energy Charge and Oxidative Stress |
title_short | Trimetazidine Protects Against Atherosclerosis by Changing Energy Charge and Oxidative Stress |
title_sort | trimetazidine protects against atherosclerosis by changing energy charge and oxidative stress |
topic | Animal Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6266541/ https://www.ncbi.nlm.nih.gov/pubmed/30468686 http://dx.doi.org/10.12659/MSM.911317 |
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