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Enhancing the Production of the Fungal Pigment Aurofusarin in Fusarium graminearum
There is an increasing demand for products from natural sources, which includes a growing market for naturally-produced colorants. Filamentous fungi produce a vast number of chemically diverse pigments and are therefore explored as an easily accessible source. In this study we examine the positive r...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6266765/ https://www.ncbi.nlm.nih.gov/pubmed/30469367 http://dx.doi.org/10.3390/toxins10110485 |
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author | Westphal, Klaus Ringsborg Wollenberg, Rasmus Dam Herbst, Florian-Alexander Sørensen, Jens Laurids Sondergaard, Teis Esben Wimmer, Reinhard |
author_facet | Westphal, Klaus Ringsborg Wollenberg, Rasmus Dam Herbst, Florian-Alexander Sørensen, Jens Laurids Sondergaard, Teis Esben Wimmer, Reinhard |
author_sort | Westphal, Klaus Ringsborg |
collection | PubMed |
description | There is an increasing demand for products from natural sources, which includes a growing market for naturally-produced colorants. Filamentous fungi produce a vast number of chemically diverse pigments and are therefore explored as an easily accessible source. In this study we examine the positive regulatory effect of the transcription factor AurR1 on the aurofusarin gene cluster in Fusarium graminearum. Proteomic analyses showed that overexpression of AurR1 resulted in a significant increase of five of the eleven proteins belonging to the aurofusarin biosynthetic pathway. Further, the production of aurofusarin was increased more than threefold in the overexpression mutant compared to the wild type, reaching levels of 270 mg/L. In addition to biosynthesis of aurofusarin, several yet undescribed putative naphthoquinone/anthraquinone analogue compounds were observed in the overexpression mutant. Our results suggest that it is possible to enhance the aurofusarin production through genetic engineering. |
format | Online Article Text |
id | pubmed-6266765 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62667652018-12-07 Enhancing the Production of the Fungal Pigment Aurofusarin in Fusarium graminearum Westphal, Klaus Ringsborg Wollenberg, Rasmus Dam Herbst, Florian-Alexander Sørensen, Jens Laurids Sondergaard, Teis Esben Wimmer, Reinhard Toxins (Basel) Communication There is an increasing demand for products from natural sources, which includes a growing market for naturally-produced colorants. Filamentous fungi produce a vast number of chemically diverse pigments and are therefore explored as an easily accessible source. In this study we examine the positive regulatory effect of the transcription factor AurR1 on the aurofusarin gene cluster in Fusarium graminearum. Proteomic analyses showed that overexpression of AurR1 resulted in a significant increase of five of the eleven proteins belonging to the aurofusarin biosynthetic pathway. Further, the production of aurofusarin was increased more than threefold in the overexpression mutant compared to the wild type, reaching levels of 270 mg/L. In addition to biosynthesis of aurofusarin, several yet undescribed putative naphthoquinone/anthraquinone analogue compounds were observed in the overexpression mutant. Our results suggest that it is possible to enhance the aurofusarin production through genetic engineering. MDPI 2018-11-21 /pmc/articles/PMC6266765/ /pubmed/30469367 http://dx.doi.org/10.3390/toxins10110485 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Westphal, Klaus Ringsborg Wollenberg, Rasmus Dam Herbst, Florian-Alexander Sørensen, Jens Laurids Sondergaard, Teis Esben Wimmer, Reinhard Enhancing the Production of the Fungal Pigment Aurofusarin in Fusarium graminearum |
title | Enhancing the Production of the Fungal Pigment Aurofusarin in Fusarium graminearum |
title_full | Enhancing the Production of the Fungal Pigment Aurofusarin in Fusarium graminearum |
title_fullStr | Enhancing the Production of the Fungal Pigment Aurofusarin in Fusarium graminearum |
title_full_unstemmed | Enhancing the Production of the Fungal Pigment Aurofusarin in Fusarium graminearum |
title_short | Enhancing the Production of the Fungal Pigment Aurofusarin in Fusarium graminearum |
title_sort | enhancing the production of the fungal pigment aurofusarin in fusarium graminearum |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6266765/ https://www.ncbi.nlm.nih.gov/pubmed/30469367 http://dx.doi.org/10.3390/toxins10110485 |
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