Cargando…

Different Biological Pathways Are Up-regulated in the Elderly With Asthma: Sputum Transcriptomic Analysis

BACKGROUND: Elderly asthma (EA) is increasing, but the pathogenesis is unclear. This study aimed to identify EA-related biological pathways by analyzing genome-wide gene expression profiles in sputum cells. METHODS: A total of 3,156 gene probes with significantly differential expressions between EA...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Byung-Keun, Lee, Hyun-Seung, Sohn, Kyoung-Hee, Lee, Suh-young, Cho, Sang-Heon, Park, Heung-Woo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Academy of Asthma, Allergy and Clinical Immunology; The Korean Academy of Pediatric Allergy and Respiratory Disease 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6267191/
https://www.ncbi.nlm.nih.gov/pubmed/30479081
http://dx.doi.org/10.4168/aair.2019.11.1.104
_version_ 1783376010729750528
author Kim, Byung-Keun
Lee, Hyun-Seung
Sohn, Kyoung-Hee
Lee, Suh-young
Cho, Sang-Heon
Park, Heung-Woo
author_facet Kim, Byung-Keun
Lee, Hyun-Seung
Sohn, Kyoung-Hee
Lee, Suh-young
Cho, Sang-Heon
Park, Heung-Woo
author_sort Kim, Byung-Keun
collection PubMed
description BACKGROUND: Elderly asthma (EA) is increasing, but the pathogenesis is unclear. This study aimed to identify EA-related biological pathways by analyzing genome-wide gene expression profiles in sputum cells. METHODS: A total of 3,156 gene probes with significantly differential expressions between EA and healthy elderly controls were used for a hierarchical clustering of genes to identify gene clusters. Gene set enrichment analysis provided biological information, with replication from Gene Expression Omnibus expression profiles. RESULTS: Fifty-five EA patients and 10 elderly control subjects were enrolled. Two distinct gene clusters were found. Cluster 1 (n = 35) showed a lower eosinophil proportion in sputum and less severe airway obstruction compared to cluster 2 (n = 20). The replication data set also identified 2 gene clusters (clusters 1' and 2'). Among 5 gene sets significantly enriched in cluster 1 and 3 gene sets significantly enriched in cluster 2, we confirmed that 2 were significantly enriched in the replication data set (OXIDATIVE_PHOSPHORYLATION gene set in cluster 1 and EPITHELIAL MESENCHYMAL TRANSITION gene set in cluster 2'). CONCLUSIONS: The findings of 2 distinct gene clusters in EA and different biological pathways in each gene cluster suggest 2 different pathogenesis mechanisms underlying EA.
format Online
Article
Text
id pubmed-6267191
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher The Korean Academy of Asthma, Allergy and Clinical Immunology; The Korean Academy of Pediatric Allergy and Respiratory Disease
record_format MEDLINE/PubMed
spelling pubmed-62671912019-01-01 Different Biological Pathways Are Up-regulated in the Elderly With Asthma: Sputum Transcriptomic Analysis Kim, Byung-Keun Lee, Hyun-Seung Sohn, Kyoung-Hee Lee, Suh-young Cho, Sang-Heon Park, Heung-Woo Allergy Asthma Immunol Res Original Article BACKGROUND: Elderly asthma (EA) is increasing, but the pathogenesis is unclear. This study aimed to identify EA-related biological pathways by analyzing genome-wide gene expression profiles in sputum cells. METHODS: A total of 3,156 gene probes with significantly differential expressions between EA and healthy elderly controls were used for a hierarchical clustering of genes to identify gene clusters. Gene set enrichment analysis provided biological information, with replication from Gene Expression Omnibus expression profiles. RESULTS: Fifty-five EA patients and 10 elderly control subjects were enrolled. Two distinct gene clusters were found. Cluster 1 (n = 35) showed a lower eosinophil proportion in sputum and less severe airway obstruction compared to cluster 2 (n = 20). The replication data set also identified 2 gene clusters (clusters 1' and 2'). Among 5 gene sets significantly enriched in cluster 1 and 3 gene sets significantly enriched in cluster 2, we confirmed that 2 were significantly enriched in the replication data set (OXIDATIVE_PHOSPHORYLATION gene set in cluster 1 and EPITHELIAL MESENCHYMAL TRANSITION gene set in cluster 2'). CONCLUSIONS: The findings of 2 distinct gene clusters in EA and different biological pathways in each gene cluster suggest 2 different pathogenesis mechanisms underlying EA. The Korean Academy of Asthma, Allergy and Clinical Immunology; The Korean Academy of Pediatric Allergy and Respiratory Disease 2018-11-05 /pmc/articles/PMC6267191/ /pubmed/30479081 http://dx.doi.org/10.4168/aair.2019.11.1.104 Text en Copyright © 2019 The Korean Academy of Asthma, Allergy and Clinical Immunology • The Korean Academy of Pediatric Allergy and Respiratory Disease https://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kim, Byung-Keun
Lee, Hyun-Seung
Sohn, Kyoung-Hee
Lee, Suh-young
Cho, Sang-Heon
Park, Heung-Woo
Different Biological Pathways Are Up-regulated in the Elderly With Asthma: Sputum Transcriptomic Analysis
title Different Biological Pathways Are Up-regulated in the Elderly With Asthma: Sputum Transcriptomic Analysis
title_full Different Biological Pathways Are Up-regulated in the Elderly With Asthma: Sputum Transcriptomic Analysis
title_fullStr Different Biological Pathways Are Up-regulated in the Elderly With Asthma: Sputum Transcriptomic Analysis
title_full_unstemmed Different Biological Pathways Are Up-regulated in the Elderly With Asthma: Sputum Transcriptomic Analysis
title_short Different Biological Pathways Are Up-regulated in the Elderly With Asthma: Sputum Transcriptomic Analysis
title_sort different biological pathways are up-regulated in the elderly with asthma: sputum transcriptomic analysis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6267191/
https://www.ncbi.nlm.nih.gov/pubmed/30479081
http://dx.doi.org/10.4168/aair.2019.11.1.104
work_keys_str_mv AT kimbyungkeun differentbiologicalpathwaysareupregulatedintheelderlywithasthmasputumtranscriptomicanalysis
AT leehyunseung differentbiologicalpathwaysareupregulatedintheelderlywithasthmasputumtranscriptomicanalysis
AT sohnkyounghee differentbiologicalpathwaysareupregulatedintheelderlywithasthmasputumtranscriptomicanalysis
AT leesuhyoung differentbiologicalpathwaysareupregulatedintheelderlywithasthmasputumtranscriptomicanalysis
AT chosangheon differentbiologicalpathwaysareupregulatedintheelderlywithasthmasputumtranscriptomicanalysis
AT parkheungwoo differentbiologicalpathwaysareupregulatedintheelderlywithasthmasputumtranscriptomicanalysis