A novel protocol for the preparation of active recombinant human pancreatic lipase from Escherichia coli

An active recombinant human pancreatic lipase (recHPL) was successfully prepared for the first time from the Escherichia coli expression system using short Strep-tag II (ST II). The recHPL–ST II was solubilized using 8 M urea from E.coli lysate and purified on a Strep-Tactin-Sepharose column. After...

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Detalles Bibliográficos
Autores principales: Kawaguchi, Nanami, Date, Kimie, Suzuki, Yusuke, Tomita, Chihiro, Naradate, Rina, Higami, Tomoko, Nakamura, Kosuke, Aikawa, Kyoko, Ogawa, Haruko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Regular Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6267337/
https://www.ncbi.nlm.nih.gov/pubmed/30101295
http://dx.doi.org/10.1093/jb/mvy067

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