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How high resolution 3-dimensional imaging changes our understanding of postnatal lung development

During the last 10 + years biologically and clinically significant questions about postnatal lung development could be answered due to the application of modern cutting-edge microscopic and quantitative histological techniques. These are in particular synchrotron radiation based X-ray tomographic mi...

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Autor principal: Schittny, Johannes C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6267404/
https://www.ncbi.nlm.nih.gov/pubmed/30390117
http://dx.doi.org/10.1007/s00418-018-1749-7
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author Schittny, Johannes C.
author_facet Schittny, Johannes C.
author_sort Schittny, Johannes C.
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description During the last 10 + years biologically and clinically significant questions about postnatal lung development could be answered due to the application of modern cutting-edge microscopic and quantitative histological techniques. These are in particular synchrotron radiation based X-ray tomographic microscopy (SRXTM), but also (3)Helium Magnetic Resonance Imaging, as well as the stereological estimation of the number of alveoli and the length of the free septal edge. First, the most important new finding may be the following: alveolarization of the lung does not cease after the maturation of the alveolar microvasculature but continues until young adulthood and, even more important, maybe reactivated lifelong if needed to rescue structural damages of the lungs. Second, the pulmonary acinus represents the functional unit of the lung. Because the borders of the acini could not be detected in classical histological sections, any investigation of the acini requires 3-dimensional (imaging) methods. Based on SRXTM it was shown that in rat lungs the number of acini stays constant, meaning that their volume increases by a factor of ~ 11 after birth. The latter is very important for acinar ventilation and particle deposition. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00418-018-1749-7) contains supplementary material, which is available to authorized users.
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spelling pubmed-62674042018-12-11 How high resolution 3-dimensional imaging changes our understanding of postnatal lung development Schittny, Johannes C. Histochem Cell Biol Review During the last 10 + years biologically and clinically significant questions about postnatal lung development could be answered due to the application of modern cutting-edge microscopic and quantitative histological techniques. These are in particular synchrotron radiation based X-ray tomographic microscopy (SRXTM), but also (3)Helium Magnetic Resonance Imaging, as well as the stereological estimation of the number of alveoli and the length of the free septal edge. First, the most important new finding may be the following: alveolarization of the lung does not cease after the maturation of the alveolar microvasculature but continues until young adulthood and, even more important, maybe reactivated lifelong if needed to rescue structural damages of the lungs. Second, the pulmonary acinus represents the functional unit of the lung. Because the borders of the acini could not be detected in classical histological sections, any investigation of the acini requires 3-dimensional (imaging) methods. Based on SRXTM it was shown that in rat lungs the number of acini stays constant, meaning that their volume increases by a factor of ~ 11 after birth. The latter is very important for acinar ventilation and particle deposition. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00418-018-1749-7) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2018-11-02 2018 /pmc/articles/PMC6267404/ /pubmed/30390117 http://dx.doi.org/10.1007/s00418-018-1749-7 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Review
Schittny, Johannes C.
How high resolution 3-dimensional imaging changes our understanding of postnatal lung development
title How high resolution 3-dimensional imaging changes our understanding of postnatal lung development
title_full How high resolution 3-dimensional imaging changes our understanding of postnatal lung development
title_fullStr How high resolution 3-dimensional imaging changes our understanding of postnatal lung development
title_full_unstemmed How high resolution 3-dimensional imaging changes our understanding of postnatal lung development
title_short How high resolution 3-dimensional imaging changes our understanding of postnatal lung development
title_sort how high resolution 3-dimensional imaging changes our understanding of postnatal lung development
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6267404/
https://www.ncbi.nlm.nih.gov/pubmed/30390117
http://dx.doi.org/10.1007/s00418-018-1749-7
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