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How high resolution 3-dimensional imaging changes our understanding of postnatal lung development
During the last 10 + years biologically and clinically significant questions about postnatal lung development could be answered due to the application of modern cutting-edge microscopic and quantitative histological techniques. These are in particular synchrotron radiation based X-ray tomographic mi...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Springer Berlin Heidelberg
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6267404/ https://www.ncbi.nlm.nih.gov/pubmed/30390117 http://dx.doi.org/10.1007/s00418-018-1749-7 |
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author | Schittny, Johannes C. |
author_facet | Schittny, Johannes C. |
author_sort | Schittny, Johannes C. |
collection | PubMed |
description | During the last 10 + years biologically and clinically significant questions about postnatal lung development could be answered due to the application of modern cutting-edge microscopic and quantitative histological techniques. These are in particular synchrotron radiation based X-ray tomographic microscopy (SRXTM), but also (3)Helium Magnetic Resonance Imaging, as well as the stereological estimation of the number of alveoli and the length of the free septal edge. First, the most important new finding may be the following: alveolarization of the lung does not cease after the maturation of the alveolar microvasculature but continues until young adulthood and, even more important, maybe reactivated lifelong if needed to rescue structural damages of the lungs. Second, the pulmonary acinus represents the functional unit of the lung. Because the borders of the acini could not be detected in classical histological sections, any investigation of the acini requires 3-dimensional (imaging) methods. Based on SRXTM it was shown that in rat lungs the number of acini stays constant, meaning that their volume increases by a factor of ~ 11 after birth. The latter is very important for acinar ventilation and particle deposition. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00418-018-1749-7) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6267404 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-62674042018-12-11 How high resolution 3-dimensional imaging changes our understanding of postnatal lung development Schittny, Johannes C. Histochem Cell Biol Review During the last 10 + years biologically and clinically significant questions about postnatal lung development could be answered due to the application of modern cutting-edge microscopic and quantitative histological techniques. These are in particular synchrotron radiation based X-ray tomographic microscopy (SRXTM), but also (3)Helium Magnetic Resonance Imaging, as well as the stereological estimation of the number of alveoli and the length of the free septal edge. First, the most important new finding may be the following: alveolarization of the lung does not cease after the maturation of the alveolar microvasculature but continues until young adulthood and, even more important, maybe reactivated lifelong if needed to rescue structural damages of the lungs. Second, the pulmonary acinus represents the functional unit of the lung. Because the borders of the acini could not be detected in classical histological sections, any investigation of the acini requires 3-dimensional (imaging) methods. Based on SRXTM it was shown that in rat lungs the number of acini stays constant, meaning that their volume increases by a factor of ~ 11 after birth. The latter is very important for acinar ventilation and particle deposition. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00418-018-1749-7) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2018-11-02 2018 /pmc/articles/PMC6267404/ /pubmed/30390117 http://dx.doi.org/10.1007/s00418-018-1749-7 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Review Schittny, Johannes C. How high resolution 3-dimensional imaging changes our understanding of postnatal lung development |
title | How high resolution 3-dimensional imaging changes our understanding of postnatal lung development |
title_full | How high resolution 3-dimensional imaging changes our understanding of postnatal lung development |
title_fullStr | How high resolution 3-dimensional imaging changes our understanding of postnatal lung development |
title_full_unstemmed | How high resolution 3-dimensional imaging changes our understanding of postnatal lung development |
title_short | How high resolution 3-dimensional imaging changes our understanding of postnatal lung development |
title_sort | how high resolution 3-dimensional imaging changes our understanding of postnatal lung development |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6267404/ https://www.ncbi.nlm.nih.gov/pubmed/30390117 http://dx.doi.org/10.1007/s00418-018-1749-7 |
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