Cargando…
LncRNA ANCR promotes proliferation and radiation resistance of nasopharyngeal carcinoma by inhibiting PTEN expression
INTRODUCTION: Antidifferentiation noncoding RNA (ANCR) is a newly identified long noncoding RNA, which is reported to function as an oncogene in multiple human cancers. However, its function in nasopharyngeal carcinoma (NPC) and underlying mechanism are still unclear. MATERIALS AND METHODS: We explo...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6267624/ https://www.ncbi.nlm.nih.gov/pubmed/30568463 http://dx.doi.org/10.2147/OTT.S182573 |
_version_ | 1783376117633122304 |
---|---|
author | Ma, Xingkai Zhou, Jieyu Liu, Jianyong Wu, Geping Yu, Yan Zhu, Hongyan Liu, Jisheng |
author_facet | Ma, Xingkai Zhou, Jieyu Liu, Jianyong Wu, Geping Yu, Yan Zhu, Hongyan Liu, Jisheng |
author_sort | Ma, Xingkai |
collection | PubMed |
description | INTRODUCTION: Antidifferentiation noncoding RNA (ANCR) is a newly identified long noncoding RNA, which is reported to function as an oncogene in multiple human cancers. However, its function in nasopharyngeal carcinoma (NPC) and underlying mechanism are still unclear. MATERIALS AND METHODS: We explored the expression of ANCR in NPC tissues and cells by real-time PCR and analyzed the relationship between ANCR expression and clinicopathological characteristics of NPC patients by Pearson’s chi-squared test. Then we inhibited ANCR expression in NPC cells using siRNAs and evaluated the effect of ANCR expression on cell proliferation, colony formation, and radiosensitivity by cell counting kit-8 assay and colony formation assay. We used RT-PCR and Western blot analyses to search target genes of ANCR. Also, we used RNA immunoprecipitation (RIP) assay and chromatin immunoprecipitation assay to study the molecular mechanism in this regulation. RESULTS: We found that ANCR was upregulated in NPC tissues and cells. ANCR expression was significantly correlated with tumor size and TNM stage. Further, ANCR knockdown inhibited NPC cell growth and radiation resistance. Mechanistically, we found that PTEN was upregulated in ANCR knockdown NPC cells. In addition, RIP assay indicated that EZH2, the oncogenic histone methyltransferase of polycomb repressive complex 2, interacted with ANCR in NPC cells. More importantly, the binding of EZH2 and deposition of relevant negative histone marker H3K27me3 on PTEN promoter depended on ANCR expression. CONCLUSION: ANCR expression is upregulated in NPC and promotes NPC growth and radiation resistance through an epigenetic regulation of PTEN expression. |
format | Online Article Text |
id | pubmed-6267624 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-62676242018-12-19 LncRNA ANCR promotes proliferation and radiation resistance of nasopharyngeal carcinoma by inhibiting PTEN expression Ma, Xingkai Zhou, Jieyu Liu, Jianyong Wu, Geping Yu, Yan Zhu, Hongyan Liu, Jisheng Onco Targets Ther Original Research INTRODUCTION: Antidifferentiation noncoding RNA (ANCR) is a newly identified long noncoding RNA, which is reported to function as an oncogene in multiple human cancers. However, its function in nasopharyngeal carcinoma (NPC) and underlying mechanism are still unclear. MATERIALS AND METHODS: We explored the expression of ANCR in NPC tissues and cells by real-time PCR and analyzed the relationship between ANCR expression and clinicopathological characteristics of NPC patients by Pearson’s chi-squared test. Then we inhibited ANCR expression in NPC cells using siRNAs and evaluated the effect of ANCR expression on cell proliferation, colony formation, and radiosensitivity by cell counting kit-8 assay and colony formation assay. We used RT-PCR and Western blot analyses to search target genes of ANCR. Also, we used RNA immunoprecipitation (RIP) assay and chromatin immunoprecipitation assay to study the molecular mechanism in this regulation. RESULTS: We found that ANCR was upregulated in NPC tissues and cells. ANCR expression was significantly correlated with tumor size and TNM stage. Further, ANCR knockdown inhibited NPC cell growth and radiation resistance. Mechanistically, we found that PTEN was upregulated in ANCR knockdown NPC cells. In addition, RIP assay indicated that EZH2, the oncogenic histone methyltransferase of polycomb repressive complex 2, interacted with ANCR in NPC cells. More importantly, the binding of EZH2 and deposition of relevant negative histone marker H3K27me3 on PTEN promoter depended on ANCR expression. CONCLUSION: ANCR expression is upregulated in NPC and promotes NPC growth and radiation resistance through an epigenetic regulation of PTEN expression. Dove Medical Press 2018-11-27 /pmc/articles/PMC6267624/ /pubmed/30568463 http://dx.doi.org/10.2147/OTT.S182573 Text en © 2018 Ma et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Ma, Xingkai Zhou, Jieyu Liu, Jianyong Wu, Geping Yu, Yan Zhu, Hongyan Liu, Jisheng LncRNA ANCR promotes proliferation and radiation resistance of nasopharyngeal carcinoma by inhibiting PTEN expression |
title | LncRNA ANCR promotes proliferation and radiation resistance of nasopharyngeal carcinoma by inhibiting PTEN expression |
title_full | LncRNA ANCR promotes proliferation and radiation resistance of nasopharyngeal carcinoma by inhibiting PTEN expression |
title_fullStr | LncRNA ANCR promotes proliferation and radiation resistance of nasopharyngeal carcinoma by inhibiting PTEN expression |
title_full_unstemmed | LncRNA ANCR promotes proliferation and radiation resistance of nasopharyngeal carcinoma by inhibiting PTEN expression |
title_short | LncRNA ANCR promotes proliferation and radiation resistance of nasopharyngeal carcinoma by inhibiting PTEN expression |
title_sort | lncrna ancr promotes proliferation and radiation resistance of nasopharyngeal carcinoma by inhibiting pten expression |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6267624/ https://www.ncbi.nlm.nih.gov/pubmed/30568463 http://dx.doi.org/10.2147/OTT.S182573 |
work_keys_str_mv | AT maxingkai lncrnaancrpromotesproliferationandradiationresistanceofnasopharyngealcarcinomabyinhibitingptenexpression AT zhoujieyu lncrnaancrpromotesproliferationandradiationresistanceofnasopharyngealcarcinomabyinhibitingptenexpression AT liujianyong lncrnaancrpromotesproliferationandradiationresistanceofnasopharyngealcarcinomabyinhibitingptenexpression AT wugeping lncrnaancrpromotesproliferationandradiationresistanceofnasopharyngealcarcinomabyinhibitingptenexpression AT yuyan lncrnaancrpromotesproliferationandradiationresistanceofnasopharyngealcarcinomabyinhibitingptenexpression AT zhuhongyan lncrnaancrpromotesproliferationandradiationresistanceofnasopharyngealcarcinomabyinhibitingptenexpression AT liujisheng lncrnaancrpromotesproliferationandradiationresistanceofnasopharyngealcarcinomabyinhibitingptenexpression |