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Platelet-Activating Factor (PAF) Antagonistic Activity of a New Biflavonoid from Garcinia nervosa var. pubescens King

The methanol extract of the leaves of Garcinia nervosa var. pubescens King, which showed strong inhibitory effects on platelet-activating factor (PAF) receptor binding, was subjected to bioassay-guided isolation to obtain a new biflavonoid, II-3,I-5, II-5,II-7,I-4',II-4'-hexahydroxy-(I-3,I...

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Detalles Bibliográficos
Autores principales: Jalil, Juriyati, Jantan, Ibrahim, Ghani, Azura Abdul, Murad, Shahnaz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268030/
https://www.ncbi.nlm.nih.gov/pubmed/22964504
http://dx.doi.org/10.3390/molecules170910893
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author Jalil, Juriyati
Jantan, Ibrahim
Ghani, Azura Abdul
Murad, Shahnaz
author_facet Jalil, Juriyati
Jantan, Ibrahim
Ghani, Azura Abdul
Murad, Shahnaz
author_sort Jalil, Juriyati
collection PubMed
description The methanol extract of the leaves of Garcinia nervosa var. pubescens King, which showed strong inhibitory effects on platelet-activating factor (PAF) receptor binding, was subjected to bioassay-guided isolation to obtain a new biflavonoid, II-3,I-5, II-5,II-7,I-4',II-4'-hexahydroxy-(I-3,II-8)-flavonylflavanonol together with two known flavonoids, 6-methyl-4'-methoxyflavone and acacetin. The structures of the compounds were elucidated by spectroscopic methods. The compounds were evaluated for their ability to inhibit PAF receptor binding to rabbit platelets using (3)H-PAF as a ligand. The biflavonoid and acacetin showed strong inhibition with IC(50) values of 28.0 and 20.4 µM, respectively. The results suggest that these compounds could be responsible for the strong PAF antagonistic activity of the plant.
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spelling pubmed-62680302018-12-12 Platelet-Activating Factor (PAF) Antagonistic Activity of a New Biflavonoid from Garcinia nervosa var. pubescens King Jalil, Juriyati Jantan, Ibrahim Ghani, Azura Abdul Murad, Shahnaz Molecules Article The methanol extract of the leaves of Garcinia nervosa var. pubescens King, which showed strong inhibitory effects on platelet-activating factor (PAF) receptor binding, was subjected to bioassay-guided isolation to obtain a new biflavonoid, II-3,I-5, II-5,II-7,I-4',II-4'-hexahydroxy-(I-3,II-8)-flavonylflavanonol together with two known flavonoids, 6-methyl-4'-methoxyflavone and acacetin. The structures of the compounds were elucidated by spectroscopic methods. The compounds were evaluated for their ability to inhibit PAF receptor binding to rabbit platelets using (3)H-PAF as a ligand. The biflavonoid and acacetin showed strong inhibition with IC(50) values of 28.0 and 20.4 µM, respectively. The results suggest that these compounds could be responsible for the strong PAF antagonistic activity of the plant. MDPI 2012-09-10 /pmc/articles/PMC6268030/ /pubmed/22964504 http://dx.doi.org/10.3390/molecules170910893 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Jalil, Juriyati
Jantan, Ibrahim
Ghani, Azura Abdul
Murad, Shahnaz
Platelet-Activating Factor (PAF) Antagonistic Activity of a New Biflavonoid from Garcinia nervosa var. pubescens King
title Platelet-Activating Factor (PAF) Antagonistic Activity of a New Biflavonoid from Garcinia nervosa var. pubescens King
title_full Platelet-Activating Factor (PAF) Antagonistic Activity of a New Biflavonoid from Garcinia nervosa var. pubescens King
title_fullStr Platelet-Activating Factor (PAF) Antagonistic Activity of a New Biflavonoid from Garcinia nervosa var. pubescens King
title_full_unstemmed Platelet-Activating Factor (PAF) Antagonistic Activity of a New Biflavonoid from Garcinia nervosa var. pubescens King
title_short Platelet-Activating Factor (PAF) Antagonistic Activity of a New Biflavonoid from Garcinia nervosa var. pubescens King
title_sort platelet-activating factor (paf) antagonistic activity of a new biflavonoid from garcinia nervosa var. pubescens king
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268030/
https://www.ncbi.nlm.nih.gov/pubmed/22964504
http://dx.doi.org/10.3390/molecules170910893
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