Purification, Characterization and Antioxidant Activities in Vitro and in Vivo of the Polysaccharides from Boletus edulis Bull

A water-soluble polysaccharide (BEBP) was extracted from Boletus edulis Bull using hot water extraction followed by ethanol precipitation. The polysaccharide BEBP was further purified by chromatography on a DEAE-cellulose column, giving three major polysaccharide fractions termed BEBP-1, BEBP-2 and...

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Detalles Bibliográficos
Autores principales: Luo, Aoxue, Luo, Aoshuang, Huang, Jiandong, Fan, Yijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268106/
https://www.ncbi.nlm.nih.gov/pubmed/22766800
http://dx.doi.org/10.3390/molecules17078079
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author Luo, Aoxue
Luo, Aoshuang
Huang, Jiandong
Fan, Yijun
author_facet Luo, Aoxue
Luo, Aoshuang
Huang, Jiandong
Fan, Yijun
author_sort Luo, Aoxue
collection PubMed
description A water-soluble polysaccharide (BEBP) was extracted from Boletus edulis Bull using hot water extraction followed by ethanol precipitation. The polysaccharide BEBP was further purified by chromatography on a DEAE-cellulose column, giving three major polysaccharide fractions termed BEBP-1, BEBP-2 and BEBP-3. In the next experiment, the average molecular weight (Mw), IR and monosaccharide compositional analysis of the three polysaccharide fractions were determined. The evaluation of antioxidant activities both in vitro and in vivo suggested that BEBP-3 had good potential antioxidant activity, and should be explored as a novel potential antioxidant.
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spelling pubmed-62681062018-12-12 Purification, Characterization and Antioxidant Activities in Vitro and in Vivo of the Polysaccharides from Boletus edulis Bull Luo, Aoxue Luo, Aoshuang Huang, Jiandong Fan, Yijun Molecules Article A water-soluble polysaccharide (BEBP) was extracted from Boletus edulis Bull using hot water extraction followed by ethanol precipitation. The polysaccharide BEBP was further purified by chromatography on a DEAE-cellulose column, giving three major polysaccharide fractions termed BEBP-1, BEBP-2 and BEBP-3. In the next experiment, the average molecular weight (Mw), IR and monosaccharide compositional analysis of the three polysaccharide fractions were determined. The evaluation of antioxidant activities both in vitro and in vivo suggested that BEBP-3 had good potential antioxidant activity, and should be explored as a novel potential antioxidant. MDPI 2012-07-05 /pmc/articles/PMC6268106/ /pubmed/22766800 http://dx.doi.org/10.3390/molecules17078079 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Luo, Aoxue
Luo, Aoshuang
Huang, Jiandong
Fan, Yijun
Purification, Characterization and Antioxidant Activities in Vitro and in Vivo of the Polysaccharides from Boletus edulis Bull
title Purification, Characterization and Antioxidant Activities in Vitro and in Vivo of the Polysaccharides from Boletus edulis Bull
title_full Purification, Characterization and Antioxidant Activities in Vitro and in Vivo of the Polysaccharides from Boletus edulis Bull
title_fullStr Purification, Characterization and Antioxidant Activities in Vitro and in Vivo of the Polysaccharides from Boletus edulis Bull
title_full_unstemmed Purification, Characterization and Antioxidant Activities in Vitro and in Vivo of the Polysaccharides from Boletus edulis Bull
title_short Purification, Characterization and Antioxidant Activities in Vitro and in Vivo of the Polysaccharides from Boletus edulis Bull
title_sort purification, characterization and antioxidant activities in vitro and in vivo of the polysaccharides from boletus edulis bull
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268106/
https://www.ncbi.nlm.nih.gov/pubmed/22766800
http://dx.doi.org/10.3390/molecules17078079
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