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Action of Chitosan Against Xanthomonas Pathogenic Bacteria Isolated from Euphorbia pulcherrima

The antibacterial activity and mechanism of two kinds of chitosan were investigated against twelve Xanthomonas strains recovered from Euphorbia pulcherrima. Results indicated that both chitosans markedly inhibited bacterial growth based on OD loss. Furthermore, the release of DNA and RNA from three...

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Detalles Bibliográficos
Autores principales: Wang, Yanli, Li, Liping, Li, Bin, Wu, Guoxing, Tang, Qiaomei, Ibrahim, Muhammad, Li, Hongye, Xie, Guanlin, Sun, Guochang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268448/
https://www.ncbi.nlm.nih.gov/pubmed/22678416
http://dx.doi.org/10.3390/molecules17067028
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author Wang, Yanli
Li, Liping
Li, Bin
Wu, Guoxing
Tang, Qiaomei
Ibrahim, Muhammad
Li, Hongye
Xie, Guanlin
Sun, Guochang
author_facet Wang, Yanli
Li, Liping
Li, Bin
Wu, Guoxing
Tang, Qiaomei
Ibrahim, Muhammad
Li, Hongye
Xie, Guanlin
Sun, Guochang
author_sort Wang, Yanli
collection PubMed
description The antibacterial activity and mechanism of two kinds of chitosan were investigated against twelve Xanthomonas strains recovered from Euphorbia pulcherrima. Results indicated that both chitosans markedly inhibited bacterial growth based on OD loss. Furthermore, the release of DNA and RNA from three selected strains was increased by both chitosans. However, the release of intracellular proteins was inhibited by both chitosans at different concentration and incubation times, except chitosan A at 0.1 mg/mL for 0.5 h incubation and 0.2 mg/mL for 2.0 h incubation increased the release of proteins, indicating the complexity of the interaction and cell membranes, which was affected by incubation time, bacterial species, chitosan type and concentration. Transmission electron microscopic observations revealed that chitosan caused changes in protoplast concentration and surface morphology. In some cells, the membranes and walls were badly distorted and disrupted, while other cells were enveloped by a thick and compact ribbon-like layer. The contrary influence on cell morphology may explain the differential effect in the release of material. In addition, scanning electron microscope and biofilm formation test revealed that both chitosans removed biofilm biomass. Overall, this study showed that membrane and biofilm play an important role in the antibacterial mechanism of chitosan.
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spelling pubmed-62684482018-12-12 Action of Chitosan Against Xanthomonas Pathogenic Bacteria Isolated from Euphorbia pulcherrima Wang, Yanli Li, Liping Li, Bin Wu, Guoxing Tang, Qiaomei Ibrahim, Muhammad Li, Hongye Xie, Guanlin Sun, Guochang Molecules Article The antibacterial activity and mechanism of two kinds of chitosan were investigated against twelve Xanthomonas strains recovered from Euphorbia pulcherrima. Results indicated that both chitosans markedly inhibited bacterial growth based on OD loss. Furthermore, the release of DNA and RNA from three selected strains was increased by both chitosans. However, the release of intracellular proteins was inhibited by both chitosans at different concentration and incubation times, except chitosan A at 0.1 mg/mL for 0.5 h incubation and 0.2 mg/mL for 2.0 h incubation increased the release of proteins, indicating the complexity of the interaction and cell membranes, which was affected by incubation time, bacterial species, chitosan type and concentration. Transmission electron microscopic observations revealed that chitosan caused changes in protoplast concentration and surface morphology. In some cells, the membranes and walls were badly distorted and disrupted, while other cells were enveloped by a thick and compact ribbon-like layer. The contrary influence on cell morphology may explain the differential effect in the release of material. In addition, scanning electron microscope and biofilm formation test revealed that both chitosans removed biofilm biomass. Overall, this study showed that membrane and biofilm play an important role in the antibacterial mechanism of chitosan. MDPI 2012-06-07 /pmc/articles/PMC6268448/ /pubmed/22678416 http://dx.doi.org/10.3390/molecules17067028 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Wang, Yanli
Li, Liping
Li, Bin
Wu, Guoxing
Tang, Qiaomei
Ibrahim, Muhammad
Li, Hongye
Xie, Guanlin
Sun, Guochang
Action of Chitosan Against Xanthomonas Pathogenic Bacteria Isolated from Euphorbia pulcherrima
title Action of Chitosan Against Xanthomonas Pathogenic Bacteria Isolated from Euphorbia pulcherrima
title_full Action of Chitosan Against Xanthomonas Pathogenic Bacteria Isolated from Euphorbia pulcherrima
title_fullStr Action of Chitosan Against Xanthomonas Pathogenic Bacteria Isolated from Euphorbia pulcherrima
title_full_unstemmed Action of Chitosan Against Xanthomonas Pathogenic Bacteria Isolated from Euphorbia pulcherrima
title_short Action of Chitosan Against Xanthomonas Pathogenic Bacteria Isolated from Euphorbia pulcherrima
title_sort action of chitosan against xanthomonas pathogenic bacteria isolated from euphorbia pulcherrima
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268448/
https://www.ncbi.nlm.nih.gov/pubmed/22678416
http://dx.doi.org/10.3390/molecules17067028
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