Development and Characterization of EST-SSR Markers from Scapharca broughtonii and Their Transferability in Scapharca subcrenata and Tegillarca granosa

Twenty-five novel EST-derived simple sequence repeat (EST-SSR) markers were developed in the ark shell Scapharca broughtonii. Polymorphisms of these EST-SSR markers were evaluated in 48 wild individuals collected from Shidao, Shandong Province, China. A total of 202 alleles were detected at 25 loci....

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Autores principales: Li, Meng, Zhu, Ling, Zhou, Chun-Ya, Lin, Lin, Fan, Yan-Jun, Zhuang, Zhi-Meng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268455/
https://www.ncbi.nlm.nih.gov/pubmed/22960866
http://dx.doi.org/10.3390/molecules170910716
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author Li, Meng
Zhu, Ling
Zhou, Chun-Ya
Lin, Lin
Fan, Yan-Jun
Zhuang, Zhi-Meng
author_facet Li, Meng
Zhu, Ling
Zhou, Chun-Ya
Lin, Lin
Fan, Yan-Jun
Zhuang, Zhi-Meng
author_sort Li, Meng
collection PubMed
description Twenty-five novel EST-derived simple sequence repeat (EST-SSR) markers were developed in the ark shell Scapharca broughtonii. Polymorphisms of these EST-SSR markers were evaluated in 48 wild individuals collected from Shidao, Shandong Province, China. A total of 202 alleles were detected at 25 loci. The numbers of alleles per locus ranged from 4 to 14, with an average of 8.08. The observed and expected heterozygosities varied from 0.2917 to 1.000 and from 0.3570 to 0.9002, respectively. After sequential Bonferroni correction for multiple tests, only one locus was found to deviate from Hardy-Weinberg equilibrium. Twenty-five EST-SSR markers showed a high rate of across-species transferability (100%) in Scapharca subcrenata and a low rate of across-genus transferability (20%) in Tegillarca granosa. These EST-SSRs will be helpful for QTL mapping, molecular breeding and investigation of population genetic diversity in ark shell S. broughtonii and other Scapharca species.
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spelling pubmed-62684552018-12-12 Development and Characterization of EST-SSR Markers from Scapharca broughtonii and Their Transferability in Scapharca subcrenata and Tegillarca granosa Li, Meng Zhu, Ling Zhou, Chun-Ya Lin, Lin Fan, Yan-Jun Zhuang, Zhi-Meng Molecules Article Twenty-five novel EST-derived simple sequence repeat (EST-SSR) markers were developed in the ark shell Scapharca broughtonii. Polymorphisms of these EST-SSR markers were evaluated in 48 wild individuals collected from Shidao, Shandong Province, China. A total of 202 alleles were detected at 25 loci. The numbers of alleles per locus ranged from 4 to 14, with an average of 8.08. The observed and expected heterozygosities varied from 0.2917 to 1.000 and from 0.3570 to 0.9002, respectively. After sequential Bonferroni correction for multiple tests, only one locus was found to deviate from Hardy-Weinberg equilibrium. Twenty-five EST-SSR markers showed a high rate of across-species transferability (100%) in Scapharca subcrenata and a low rate of across-genus transferability (20%) in Tegillarca granosa. These EST-SSRs will be helpful for QTL mapping, molecular breeding and investigation of population genetic diversity in ark shell S. broughtonii and other Scapharca species. MDPI 2012-09-07 /pmc/articles/PMC6268455/ /pubmed/22960866 http://dx.doi.org/10.3390/molecules170910716 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Li, Meng
Zhu, Ling
Zhou, Chun-Ya
Lin, Lin
Fan, Yan-Jun
Zhuang, Zhi-Meng
Development and Characterization of EST-SSR Markers from Scapharca broughtonii and Their Transferability in Scapharca subcrenata and Tegillarca granosa
title Development and Characterization of EST-SSR Markers from Scapharca broughtonii and Their Transferability in Scapharca subcrenata and Tegillarca granosa
title_full Development and Characterization of EST-SSR Markers from Scapharca broughtonii and Their Transferability in Scapharca subcrenata and Tegillarca granosa
title_fullStr Development and Characterization of EST-SSR Markers from Scapharca broughtonii and Their Transferability in Scapharca subcrenata and Tegillarca granosa
title_full_unstemmed Development and Characterization of EST-SSR Markers from Scapharca broughtonii and Their Transferability in Scapharca subcrenata and Tegillarca granosa
title_short Development and Characterization of EST-SSR Markers from Scapharca broughtonii and Their Transferability in Scapharca subcrenata and Tegillarca granosa
title_sort development and characterization of est-ssr markers from scapharca broughtonii and their transferability in scapharca subcrenata and tegillarca granosa
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268455/
https://www.ncbi.nlm.nih.gov/pubmed/22960866
http://dx.doi.org/10.3390/molecules170910716
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