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The Active Ingredients of Jiang-Zhi-Ning: Study of the Nelumbo nucifera Alkaloids and Their Main Bioactive Metabolites
The object of this study was to identify the major active ingredients of the Chinese Traditional Medicine Jiang-Zhi-Ning (JZN) based on the high performance liquid chromatography (HPLC) profiles of plasma samples obtained from beagle dogs at different times after intragastric administration of JZN,...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268456/ https://www.ncbi.nlm.nih.gov/pubmed/22898740 http://dx.doi.org/10.3390/molecules17089855 |
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author | Chen, Jianxin Ma, Xueling Gao, Kuo Wang, Yong Zhao, Huihui Wu, Hao Wang, Juan Xie, Hua OuYang, Yulin Luo, Liangtao Guo, Shuzhen Han, Jing Liu, Bing Wang, Wei |
author_facet | Chen, Jianxin Ma, Xueling Gao, Kuo Wang, Yong Zhao, Huihui Wu, Hao Wang, Juan Xie, Hua OuYang, Yulin Luo, Liangtao Guo, Shuzhen Han, Jing Liu, Bing Wang, Wei |
author_sort | Chen, Jianxin |
collection | PubMed |
description | The object of this study was to identify the major active ingredients of the Chinese Traditional Medicine Jiang-Zhi-Ning (JZN) based on the high performance liquid chromatography (HPLC) profiles of plasma samples obtained from beagle dogs at different times after intragastric administration of JZN, crude JZN extracts, different extracted fractions, different subfractions of the active fraction and different isolated ingredients. 2-Hydroxy-1-methoxyaporphin (2H1M), an alkaloid from Nelumbo nucifera, one of the herbs that make up JZN, was identified as the constituent showing the major pharmacodynamic effect. The major metabolites of 2H1M were analyzed and identified as N-demethyl-2-hydroxy-1-methoxyaporphine-2-O-glycuronic acid, 2-hydroxy-1-methoxy-aporphine-2-O-glycuronic acid and 2-hydroxy-1-methoxy-aporphine-2-O-sulphate. This study provided a comprehensive insight into the active components of JZN. |
format | Online Article Text |
id | pubmed-6268456 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62684562018-12-12 The Active Ingredients of Jiang-Zhi-Ning: Study of the Nelumbo nucifera Alkaloids and Their Main Bioactive Metabolites Chen, Jianxin Ma, Xueling Gao, Kuo Wang, Yong Zhao, Huihui Wu, Hao Wang, Juan Xie, Hua OuYang, Yulin Luo, Liangtao Guo, Shuzhen Han, Jing Liu, Bing Wang, Wei Molecules Article The object of this study was to identify the major active ingredients of the Chinese Traditional Medicine Jiang-Zhi-Ning (JZN) based on the high performance liquid chromatography (HPLC) profiles of plasma samples obtained from beagle dogs at different times after intragastric administration of JZN, crude JZN extracts, different extracted fractions, different subfractions of the active fraction and different isolated ingredients. 2-Hydroxy-1-methoxyaporphin (2H1M), an alkaloid from Nelumbo nucifera, one of the herbs that make up JZN, was identified as the constituent showing the major pharmacodynamic effect. The major metabolites of 2H1M were analyzed and identified as N-demethyl-2-hydroxy-1-methoxyaporphine-2-O-glycuronic acid, 2-hydroxy-1-methoxy-aporphine-2-O-glycuronic acid and 2-hydroxy-1-methoxy-aporphine-2-O-sulphate. This study provided a comprehensive insight into the active components of JZN. MDPI 2012-08-16 /pmc/articles/PMC6268456/ /pubmed/22898740 http://dx.doi.org/10.3390/molecules17089855 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Chen, Jianxin Ma, Xueling Gao, Kuo Wang, Yong Zhao, Huihui Wu, Hao Wang, Juan Xie, Hua OuYang, Yulin Luo, Liangtao Guo, Shuzhen Han, Jing Liu, Bing Wang, Wei The Active Ingredients of Jiang-Zhi-Ning: Study of the Nelumbo nucifera Alkaloids and Their Main Bioactive Metabolites |
title | The Active Ingredients of Jiang-Zhi-Ning: Study of the Nelumbo nucifera Alkaloids and Their Main Bioactive Metabolites |
title_full | The Active Ingredients of Jiang-Zhi-Ning: Study of the Nelumbo nucifera Alkaloids and Their Main Bioactive Metabolites |
title_fullStr | The Active Ingredients of Jiang-Zhi-Ning: Study of the Nelumbo nucifera Alkaloids and Their Main Bioactive Metabolites |
title_full_unstemmed | The Active Ingredients of Jiang-Zhi-Ning: Study of the Nelumbo nucifera Alkaloids and Their Main Bioactive Metabolites |
title_short | The Active Ingredients of Jiang-Zhi-Ning: Study of the Nelumbo nucifera Alkaloids and Their Main Bioactive Metabolites |
title_sort | active ingredients of jiang-zhi-ning: study of the nelumbo nucifera alkaloids and their main bioactive metabolites |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268456/ https://www.ncbi.nlm.nih.gov/pubmed/22898740 http://dx.doi.org/10.3390/molecules17089855 |
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