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Formononetin Attenuates IL-1β-Induced Apoptosis and NF-κB Activation in INS-1 Cells

Several studies suggest that the inflammation plays a role in the pathogenesis of some glucose disorders in adults. Exposure of pancreatic β-cells to cytokines, such as interleukin-1β (IL-1β), is thought to contribute to β-cell apoptosis. One important event triggered by IL-1β is induction of nitric...

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Autores principales: Wang, Yao, Zhu, Yunxia, Gao, Lu, Yin, Han, Xie, Zuoling, Wang, Dong, Zhu, Zhengqiu, Han, Xiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268495/
https://www.ncbi.nlm.nih.gov/pubmed/22922276
http://dx.doi.org/10.3390/molecules170910052
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author Wang, Yao
Zhu, Yunxia
Gao, Lu
Yin, Han
Xie, Zuoling
Wang, Dong
Zhu, Zhengqiu
Han, Xiao
author_facet Wang, Yao
Zhu, Yunxia
Gao, Lu
Yin, Han
Xie, Zuoling
Wang, Dong
Zhu, Zhengqiu
Han, Xiao
author_sort Wang, Yao
collection PubMed
description Several studies suggest that the inflammation plays a role in the pathogenesis of some glucose disorders in adults. Exposure of pancreatic β-cells to cytokines, such as interleukin-1β (IL-1β), is thought to contribute to β-cell apoptosis. One important event triggered by IL-1β is induction of nitric oxide synthase (iNOS), an enzyme that catalyzes intracellular generation of the cytotoxic free radical NO. Recent work have suggested that formononetin, as an O-methylated isoflavone found in a number of plants and herbs like Astragalus membranaceus, inhibited some pro-inflammatory cytokine production in macrophages. However, the roles of formononetin in pancreatic beta cells have not been fully established. The aim of the present study was to assess possible in vitro effects of formononetin on cell apoptosis induced by IL-1β in the rat insulinoma cell line, INS-1. Our results demonstrate that formononetin significantly prevents IL-1β-increased INS-1 cell death and blocks cytokine-induced apoptotic signaling (the reduction of Bax/Bcl-2 ratio and caspase-3 activity). Formononetin also inhibited the activation of nuclear factor-kappaB (NF-κB), which is a significant transcription factor for iNOS, so as to decease nitric oxide (NO) formation in a dose dependent manner in vitro. Our observations indicated that formononetin could protect against pancreatic β-cell apoptosis caused by IL-1β and therefore could be used in the future as a new drug improving diabetes mellitus.
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spelling pubmed-62684952018-12-12 Formononetin Attenuates IL-1β-Induced Apoptosis and NF-κB Activation in INS-1 Cells Wang, Yao Zhu, Yunxia Gao, Lu Yin, Han Xie, Zuoling Wang, Dong Zhu, Zhengqiu Han, Xiao Molecules Article Several studies suggest that the inflammation plays a role in the pathogenesis of some glucose disorders in adults. Exposure of pancreatic β-cells to cytokines, such as interleukin-1β (IL-1β), is thought to contribute to β-cell apoptosis. One important event triggered by IL-1β is induction of nitric oxide synthase (iNOS), an enzyme that catalyzes intracellular generation of the cytotoxic free radical NO. Recent work have suggested that formononetin, as an O-methylated isoflavone found in a number of plants and herbs like Astragalus membranaceus, inhibited some pro-inflammatory cytokine production in macrophages. However, the roles of formononetin in pancreatic beta cells have not been fully established. The aim of the present study was to assess possible in vitro effects of formononetin on cell apoptosis induced by IL-1β in the rat insulinoma cell line, INS-1. Our results demonstrate that formononetin significantly prevents IL-1β-increased INS-1 cell death and blocks cytokine-induced apoptotic signaling (the reduction of Bax/Bcl-2 ratio and caspase-3 activity). Formononetin also inhibited the activation of nuclear factor-kappaB (NF-κB), which is a significant transcription factor for iNOS, so as to decease nitric oxide (NO) formation in a dose dependent manner in vitro. Our observations indicated that formononetin could protect against pancreatic β-cell apoptosis caused by IL-1β and therefore could be used in the future as a new drug improving diabetes mellitus. MDPI 2012-08-24 /pmc/articles/PMC6268495/ /pubmed/22922276 http://dx.doi.org/10.3390/molecules170910052 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Wang, Yao
Zhu, Yunxia
Gao, Lu
Yin, Han
Xie, Zuoling
Wang, Dong
Zhu, Zhengqiu
Han, Xiao
Formononetin Attenuates IL-1β-Induced Apoptosis and NF-κB Activation in INS-1 Cells
title Formononetin Attenuates IL-1β-Induced Apoptosis and NF-κB Activation in INS-1 Cells
title_full Formononetin Attenuates IL-1β-Induced Apoptosis and NF-κB Activation in INS-1 Cells
title_fullStr Formononetin Attenuates IL-1β-Induced Apoptosis and NF-κB Activation in INS-1 Cells
title_full_unstemmed Formononetin Attenuates IL-1β-Induced Apoptosis and NF-κB Activation in INS-1 Cells
title_short Formononetin Attenuates IL-1β-Induced Apoptosis and NF-κB Activation in INS-1 Cells
title_sort formononetin attenuates il-1β-induced apoptosis and nf-κb activation in ins-1 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268495/
https://www.ncbi.nlm.nih.gov/pubmed/22922276
http://dx.doi.org/10.3390/molecules170910052
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