Cargando…

Phytochemical and Antioxidant-Related Investigations on Bark of Abies spectabilis (D. Don) Spach. from Nepal

The bark of several coniferous species, a waste product of the timber industry, contains significant amounts of natural antioxidants. In our ongoing studies of Nepalese medicinal plants, we examined the bark from Abies spectabilis as the starting material for extracting antioxidant compounds. In vit...

Descripción completa

Detalles Bibliográficos
Autores principales: Dall’Acqua, Stefano, Minesso, Paola, Shresta, Bharat Babu, Comai, Stefano, Jha, Pramod Kumar, Gewali, Mohan Bikram, Greco, Emanuela, Cervellati, Rinaldo, Innocenti, Gabbriella
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268527/
https://www.ncbi.nlm.nih.gov/pubmed/22318324
http://dx.doi.org/10.3390/molecules17021686
_version_ 1783376306743803904
author Dall’Acqua, Stefano
Minesso, Paola
Shresta, Bharat Babu
Comai, Stefano
Jha, Pramod Kumar
Gewali, Mohan Bikram
Greco, Emanuela
Cervellati, Rinaldo
Innocenti, Gabbriella
author_facet Dall’Acqua, Stefano
Minesso, Paola
Shresta, Bharat Babu
Comai, Stefano
Jha, Pramod Kumar
Gewali, Mohan Bikram
Greco, Emanuela
Cervellati, Rinaldo
Innocenti, Gabbriella
author_sort Dall’Acqua, Stefano
collection PubMed
description The bark of several coniferous species, a waste product of the timber industry, contains significant amounts of natural antioxidants. In our ongoing studies of Nepalese medicinal plants, we examined the bark from Abies spectabilis as the starting material for extracting antioxidant compounds. In vitro antioxidant activity evaluated by means of three antioxidant methods, namely 2,2-diphenyl-1-picrylhydrazyl (DPPH), Briggs-Rauscher oscillating reaction (BR) and Trolox Equivalent Antioxidant Capacity (TEAC) and total phenol contents with the Folin-Ciocalteau reagent; the ferrous iron chelating capacity was also assessed. The methanol extract of A. spectabilis showed significant antioxidant activity and polyphenol contents (IC(50) 4.13 µg/mL, 0.20 μg/mL eq. resorcinol, 4.22 mM eq. Trolox, 3.9 µg/g eq. gallic Acid in the DPPH, BR, TEAC and Folin-Ciocalteau tests, respectively) and weak Fe(2+) chelating capacity. Phytochemical studies were also carried out with 1D- and 2D NMR experiments and DI-ESI-MS, HPLC-DAD and LC-MSn measurements. Oligomeric C-type proanthocyanidins, mainly trimeric gallocatechin derivatives, were the most abundant compounds (16% of extract expressed as procyanindin B1). Gallocatechin oligomers (up to six units) and prodelphynidin-gallocatechin polymers were also identified in the extract. Prodelphynidin B4, cyclograndisolide and trans-docosanil ferulate were also isolated and characterized by NMR and MS spectroscopy.
format Online
Article
Text
id pubmed-6268527
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-62685272018-12-10 Phytochemical and Antioxidant-Related Investigations on Bark of Abies spectabilis (D. Don) Spach. from Nepal Dall’Acqua, Stefano Minesso, Paola Shresta, Bharat Babu Comai, Stefano Jha, Pramod Kumar Gewali, Mohan Bikram Greco, Emanuela Cervellati, Rinaldo Innocenti, Gabbriella Molecules Article The bark of several coniferous species, a waste product of the timber industry, contains significant amounts of natural antioxidants. In our ongoing studies of Nepalese medicinal plants, we examined the bark from Abies spectabilis as the starting material for extracting antioxidant compounds. In vitro antioxidant activity evaluated by means of three antioxidant methods, namely 2,2-diphenyl-1-picrylhydrazyl (DPPH), Briggs-Rauscher oscillating reaction (BR) and Trolox Equivalent Antioxidant Capacity (TEAC) and total phenol contents with the Folin-Ciocalteau reagent; the ferrous iron chelating capacity was also assessed. The methanol extract of A. spectabilis showed significant antioxidant activity and polyphenol contents (IC(50) 4.13 µg/mL, 0.20 μg/mL eq. resorcinol, 4.22 mM eq. Trolox, 3.9 µg/g eq. gallic Acid in the DPPH, BR, TEAC and Folin-Ciocalteau tests, respectively) and weak Fe(2+) chelating capacity. Phytochemical studies were also carried out with 1D- and 2D NMR experiments and DI-ESI-MS, HPLC-DAD and LC-MSn measurements. Oligomeric C-type proanthocyanidins, mainly trimeric gallocatechin derivatives, were the most abundant compounds (16% of extract expressed as procyanindin B1). Gallocatechin oligomers (up to six units) and prodelphynidin-gallocatechin polymers were also identified in the extract. Prodelphynidin B4, cyclograndisolide and trans-docosanil ferulate were also isolated and characterized by NMR and MS spectroscopy. MDPI 2012-02-08 /pmc/articles/PMC6268527/ /pubmed/22318324 http://dx.doi.org/10.3390/molecules17021686 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Dall’Acqua, Stefano
Minesso, Paola
Shresta, Bharat Babu
Comai, Stefano
Jha, Pramod Kumar
Gewali, Mohan Bikram
Greco, Emanuela
Cervellati, Rinaldo
Innocenti, Gabbriella
Phytochemical and Antioxidant-Related Investigations on Bark of Abies spectabilis (D. Don) Spach. from Nepal
title Phytochemical and Antioxidant-Related Investigations on Bark of Abies spectabilis (D. Don) Spach. from Nepal
title_full Phytochemical and Antioxidant-Related Investigations on Bark of Abies spectabilis (D. Don) Spach. from Nepal
title_fullStr Phytochemical and Antioxidant-Related Investigations on Bark of Abies spectabilis (D. Don) Spach. from Nepal
title_full_unstemmed Phytochemical and Antioxidant-Related Investigations on Bark of Abies spectabilis (D. Don) Spach. from Nepal
title_short Phytochemical and Antioxidant-Related Investigations on Bark of Abies spectabilis (D. Don) Spach. from Nepal
title_sort phytochemical and antioxidant-related investigations on bark of abies spectabilis (d. don) spach. from nepal
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268527/
https://www.ncbi.nlm.nih.gov/pubmed/22318324
http://dx.doi.org/10.3390/molecules17021686
work_keys_str_mv AT dallacquastefano phytochemicalandantioxidantrelatedinvestigationsonbarkofabiesspectabilisddonspachfromnepal
AT minessopaola phytochemicalandantioxidantrelatedinvestigationsonbarkofabiesspectabilisddonspachfromnepal
AT shrestabharatbabu phytochemicalandantioxidantrelatedinvestigationsonbarkofabiesspectabilisddonspachfromnepal
AT comaistefano phytochemicalandantioxidantrelatedinvestigationsonbarkofabiesspectabilisddonspachfromnepal
AT jhapramodkumar phytochemicalandantioxidantrelatedinvestigationsonbarkofabiesspectabilisddonspachfromnepal
AT gewalimohanbikram phytochemicalandantioxidantrelatedinvestigationsonbarkofabiesspectabilisddonspachfromnepal
AT grecoemanuela phytochemicalandantioxidantrelatedinvestigationsonbarkofabiesspectabilisddonspachfromnepal
AT cervellatirinaldo phytochemicalandantioxidantrelatedinvestigationsonbarkofabiesspectabilisddonspachfromnepal
AT innocentigabbriella phytochemicalandantioxidantrelatedinvestigationsonbarkofabiesspectabilisddonspachfromnepal