Cargando…

A Phytoanticipin Derivative, Sodium Houttuyfonate, Induces in Vitro Synergistic Effects with Levofloxacin against Biofilm Formation by Pseudomonas aeruginosa

Antibiotic resistance has become the main deadly factor in infections, as bacteria can protect themselves by hiding in a self-constructed biofilm. Consequently, more attention is being paid to the search for “non-antibiotic drugs” to solve this problem. Phytoanticipins, the natural antibiotics from...

Descripción completa

Detalles Bibliográficos
Autores principales: Shao, Jing, Cheng, Huijuan, Wang, Changzhong, Wang, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268569/
https://www.ncbi.nlm.nih.gov/pubmed/22996347
http://dx.doi.org/10.3390/molecules170911242
_version_ 1783376316553232384
author Shao, Jing
Cheng, Huijuan
Wang, Changzhong
Wang, Yan
author_facet Shao, Jing
Cheng, Huijuan
Wang, Changzhong
Wang, Yan
author_sort Shao, Jing
collection PubMed
description Antibiotic resistance has become the main deadly factor in infections, as bacteria can protect themselves by hiding in a self-constructed biofilm. Consequently, more attention is being paid to the search for “non-antibiotic drugs” to solve this problem. Phytoanticipins, the natural antibiotics from plants, could be a suitable alternative, but few works on this aspect have been reported. In this study, a preliminary study on the synergy between sodium houttuyfonate (SH) and levofloxacin (LFX) against the biofilm formation of Pseudomonas aeruginosa was performed. The minimal inhibitory concentrations (MIC) of LFX and SH, anti-biofilm formation and synergistic effect on Pseudomonas aeruginosa, and quantification of alginate were determined by the microdilution method, crystal violet (CV) assay, checkerboard method, and hydroxybiphenyl colorimetry. The biofilm morphology of Pseudomonas aeruginosa was observed by fluorescence microscope and scanning electric microscope (SEM). The results showed that: (i) LFX and SH had an obvious synergistic effect against Pseudomonas aeruginosa with MIC values of 0.25 μg/mL and 128 μg/mL, respectively; (ii) ½ × MIC SH combined with 2 × MIC LFX could suppress the biofilm formation of Pseudomonas aeruginosa effectively, with up to 73% inhibition; (iii) the concentration of alginate decreased dramatically by a maximum of 92% after treatment with the combination of antibiotics; and (iv) more dead cells by fluorescence microscope and more removal of extracellular polymeric structure (EPS) by SEM were observed after the combined treatment of LFX and SH. Our experiments demonstrate the promising future of this potent antimicrobial agent against biofilm-associated infections.
format Online
Article
Text
id pubmed-6268569
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-62685692018-12-12 A Phytoanticipin Derivative, Sodium Houttuyfonate, Induces in Vitro Synergistic Effects with Levofloxacin against Biofilm Formation by Pseudomonas aeruginosa Shao, Jing Cheng, Huijuan Wang, Changzhong Wang, Yan Molecules Article Antibiotic resistance has become the main deadly factor in infections, as bacteria can protect themselves by hiding in a self-constructed biofilm. Consequently, more attention is being paid to the search for “non-antibiotic drugs” to solve this problem. Phytoanticipins, the natural antibiotics from plants, could be a suitable alternative, but few works on this aspect have been reported. In this study, a preliminary study on the synergy between sodium houttuyfonate (SH) and levofloxacin (LFX) against the biofilm formation of Pseudomonas aeruginosa was performed. The minimal inhibitory concentrations (MIC) of LFX and SH, anti-biofilm formation and synergistic effect on Pseudomonas aeruginosa, and quantification of alginate were determined by the microdilution method, crystal violet (CV) assay, checkerboard method, and hydroxybiphenyl colorimetry. The biofilm morphology of Pseudomonas aeruginosa was observed by fluorescence microscope and scanning electric microscope (SEM). The results showed that: (i) LFX and SH had an obvious synergistic effect against Pseudomonas aeruginosa with MIC values of 0.25 μg/mL and 128 μg/mL, respectively; (ii) ½ × MIC SH combined with 2 × MIC LFX could suppress the biofilm formation of Pseudomonas aeruginosa effectively, with up to 73% inhibition; (iii) the concentration of alginate decreased dramatically by a maximum of 92% after treatment with the combination of antibiotics; and (iv) more dead cells by fluorescence microscope and more removal of extracellular polymeric structure (EPS) by SEM were observed after the combined treatment of LFX and SH. Our experiments demonstrate the promising future of this potent antimicrobial agent against biofilm-associated infections. MDPI 2012-09-20 /pmc/articles/PMC6268569/ /pubmed/22996347 http://dx.doi.org/10.3390/molecules170911242 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Shao, Jing
Cheng, Huijuan
Wang, Changzhong
Wang, Yan
A Phytoanticipin Derivative, Sodium Houttuyfonate, Induces in Vitro Synergistic Effects with Levofloxacin against Biofilm Formation by Pseudomonas aeruginosa
title A Phytoanticipin Derivative, Sodium Houttuyfonate, Induces in Vitro Synergistic Effects with Levofloxacin against Biofilm Formation by Pseudomonas aeruginosa
title_full A Phytoanticipin Derivative, Sodium Houttuyfonate, Induces in Vitro Synergistic Effects with Levofloxacin against Biofilm Formation by Pseudomonas aeruginosa
title_fullStr A Phytoanticipin Derivative, Sodium Houttuyfonate, Induces in Vitro Synergistic Effects with Levofloxacin against Biofilm Formation by Pseudomonas aeruginosa
title_full_unstemmed A Phytoanticipin Derivative, Sodium Houttuyfonate, Induces in Vitro Synergistic Effects with Levofloxacin against Biofilm Formation by Pseudomonas aeruginosa
title_short A Phytoanticipin Derivative, Sodium Houttuyfonate, Induces in Vitro Synergistic Effects with Levofloxacin against Biofilm Formation by Pseudomonas aeruginosa
title_sort phytoanticipin derivative, sodium houttuyfonate, induces in vitro synergistic effects with levofloxacin against biofilm formation by pseudomonas aeruginosa
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268569/
https://www.ncbi.nlm.nih.gov/pubmed/22996347
http://dx.doi.org/10.3390/molecules170911242
work_keys_str_mv AT shaojing aphytoanticipinderivativesodiumhouttuyfonateinducesinvitrosynergisticeffectswithlevofloxacinagainstbiofilmformationbypseudomonasaeruginosa
AT chenghuijuan aphytoanticipinderivativesodiumhouttuyfonateinducesinvitrosynergisticeffectswithlevofloxacinagainstbiofilmformationbypseudomonasaeruginosa
AT wangchangzhong aphytoanticipinderivativesodiumhouttuyfonateinducesinvitrosynergisticeffectswithlevofloxacinagainstbiofilmformationbypseudomonasaeruginosa
AT wangyan aphytoanticipinderivativesodiumhouttuyfonateinducesinvitrosynergisticeffectswithlevofloxacinagainstbiofilmformationbypseudomonasaeruginosa
AT shaojing phytoanticipinderivativesodiumhouttuyfonateinducesinvitrosynergisticeffectswithlevofloxacinagainstbiofilmformationbypseudomonasaeruginosa
AT chenghuijuan phytoanticipinderivativesodiumhouttuyfonateinducesinvitrosynergisticeffectswithlevofloxacinagainstbiofilmformationbypseudomonasaeruginosa
AT wangchangzhong phytoanticipinderivativesodiumhouttuyfonateinducesinvitrosynergisticeffectswithlevofloxacinagainstbiofilmformationbypseudomonasaeruginosa
AT wangyan phytoanticipinderivativesodiumhouttuyfonateinducesinvitrosynergisticeffectswithlevofloxacinagainstbiofilmformationbypseudomonasaeruginosa