Cargando…

Investigation on the Protective Effects of Cranberry Against the DNA Damage Induced by Benzo[a]pyrene

There are few reports that demonstrate the antigenotoxic potential of cranberries. Although the types of berry fruits consumed worldwide are many, this paper focuses on cranberries that are commonly consumed in Mexico (Vaccinium macrocarpon species). The purpose of the present study is to determine...

Descripción completa

Detalles Bibliográficos
Autores principales: Madrigal-Santillán, Eduardo, Fragoso-Antonio, Sonia, Valadez-Vega, Carmen, Solano-Solano, Gloria, Zúñiga Pérez, Clara, Sánchez-Gutiérrez, Manuel, Izquierdo-Vega, Jeannett A., Gutiérrez-Salinas, José, Esquivel-Soto, Jaime, Esquivel-Chirino, César, Sumaya-Martínez, Teresa, Fregoso-Aguilar, Tomas, Mendoza-Pérez, Jorge, Morales-González, José A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268607/
https://www.ncbi.nlm.nih.gov/pubmed/22499190
http://dx.doi.org/10.3390/molecules17044435
_version_ 1783376325512265728
author Madrigal-Santillán, Eduardo
Fragoso-Antonio, Sonia
Valadez-Vega, Carmen
Solano-Solano, Gloria
Zúñiga Pérez, Clara
Sánchez-Gutiérrez, Manuel
Izquierdo-Vega, Jeannett A.
Gutiérrez-Salinas, José
Esquivel-Soto, Jaime
Esquivel-Chirino, César
Sumaya-Martínez, Teresa
Fregoso-Aguilar, Tomas
Mendoza-Pérez, Jorge
Morales-González, José A.
author_facet Madrigal-Santillán, Eduardo
Fragoso-Antonio, Sonia
Valadez-Vega, Carmen
Solano-Solano, Gloria
Zúñiga Pérez, Clara
Sánchez-Gutiérrez, Manuel
Izquierdo-Vega, Jeannett A.
Gutiérrez-Salinas, José
Esquivel-Soto, Jaime
Esquivel-Chirino, César
Sumaya-Martínez, Teresa
Fregoso-Aguilar, Tomas
Mendoza-Pérez, Jorge
Morales-González, José A.
author_sort Madrigal-Santillán, Eduardo
collection PubMed
description There are few reports that demonstrate the antigenotoxic potential of cranberries. Although the types of berry fruits consumed worldwide are many, this paper focuses on cranberries that are commonly consumed in Mexico (Vaccinium macrocarpon species). The purpose of the present study is to determine whether cranberry ethanolic extract (CEE) can prevent the DNA damage produced by benzo[a]pyrene (B[a]P) using an in vivo mouse peripheral blood micronucleus assay. The experimental groups were organized as follows: a negative control group (without treatment), a positive group treated with B[a]P (200 mg/kg), a group administered with 800 mg/kg of CEE, and three groups treated with B[a]P and CEE (200, 400, and 800 mg/kg) respectively. The CEE and benzo[a]pyrene were administered orally for a week, on a daily basis. During this period the body weight, the feed intake, and the determination of antigenotoxic potential were quantified. At the end of this period, we continued with the same determinations for one week more (recovery period) but anymore administration of the substances. The animals treated with B[a]P showed a weight increase after the first week of administration. The same phenomenon was observed in the lots combined with B[a]P and CEE (low and medium doses). The dose of 800 mg/kg of CEE showed similar values to the control group at the end of the treatment period. In the second part of the assay, when the substances were not administered, these experimental groups regained their normal weight. The dose of CEE (800 mg/kg) was not genotoxic nor cytotoxic. On the contrary, the B[a]P increases the frequency of micronucleated normochromatic erythrocytes (MNNE) and reduces the rate of polychromatic erythrocytes (PE) at the end of the treatment period. With respect to the combined lots, a significant decrease in the MN rate was observed from the sixth to the eighth day of treatment with the two high doses applied; the highest protection (60%) was obtained with 800 mg/kg of CEE. The same dose showed an anticytotoxic effect which corresponded to an improvement of 62.5% in relation to the animals administered with the B[a]P. In the second period, all groups reached values that have been seen in the control group animals. Our results suggest that the inhibition of clastogenicity of the cranberry ethanolic extract against B[a]P is related to the antioxidant capacity of the combination of phytochemicals present in its chemical composition.
format Online
Article
Text
id pubmed-6268607
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-62686072018-12-11 Investigation on the Protective Effects of Cranberry Against the DNA Damage Induced by Benzo[a]pyrene Madrigal-Santillán, Eduardo Fragoso-Antonio, Sonia Valadez-Vega, Carmen Solano-Solano, Gloria Zúñiga Pérez, Clara Sánchez-Gutiérrez, Manuel Izquierdo-Vega, Jeannett A. Gutiérrez-Salinas, José Esquivel-Soto, Jaime Esquivel-Chirino, César Sumaya-Martínez, Teresa Fregoso-Aguilar, Tomas Mendoza-Pérez, Jorge Morales-González, José A. Molecules Article There are few reports that demonstrate the antigenotoxic potential of cranberries. Although the types of berry fruits consumed worldwide are many, this paper focuses on cranberries that are commonly consumed in Mexico (Vaccinium macrocarpon species). The purpose of the present study is to determine whether cranberry ethanolic extract (CEE) can prevent the DNA damage produced by benzo[a]pyrene (B[a]P) using an in vivo mouse peripheral blood micronucleus assay. The experimental groups were organized as follows: a negative control group (without treatment), a positive group treated with B[a]P (200 mg/kg), a group administered with 800 mg/kg of CEE, and three groups treated with B[a]P and CEE (200, 400, and 800 mg/kg) respectively. The CEE and benzo[a]pyrene were administered orally for a week, on a daily basis. During this period the body weight, the feed intake, and the determination of antigenotoxic potential were quantified. At the end of this period, we continued with the same determinations for one week more (recovery period) but anymore administration of the substances. The animals treated with B[a]P showed a weight increase after the first week of administration. The same phenomenon was observed in the lots combined with B[a]P and CEE (low and medium doses). The dose of 800 mg/kg of CEE showed similar values to the control group at the end of the treatment period. In the second part of the assay, when the substances were not administered, these experimental groups regained their normal weight. The dose of CEE (800 mg/kg) was not genotoxic nor cytotoxic. On the contrary, the B[a]P increases the frequency of micronucleated normochromatic erythrocytes (MNNE) and reduces the rate of polychromatic erythrocytes (PE) at the end of the treatment period. With respect to the combined lots, a significant decrease in the MN rate was observed from the sixth to the eighth day of treatment with the two high doses applied; the highest protection (60%) was obtained with 800 mg/kg of CEE. The same dose showed an anticytotoxic effect which corresponded to an improvement of 62.5% in relation to the animals administered with the B[a]P. In the second period, all groups reached values that have been seen in the control group animals. Our results suggest that the inhibition of clastogenicity of the cranberry ethanolic extract against B[a]P is related to the antioxidant capacity of the combination of phytochemicals present in its chemical composition. MDPI 2012-04-12 /pmc/articles/PMC6268607/ /pubmed/22499190 http://dx.doi.org/10.3390/molecules17044435 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Madrigal-Santillán, Eduardo
Fragoso-Antonio, Sonia
Valadez-Vega, Carmen
Solano-Solano, Gloria
Zúñiga Pérez, Clara
Sánchez-Gutiérrez, Manuel
Izquierdo-Vega, Jeannett A.
Gutiérrez-Salinas, José
Esquivel-Soto, Jaime
Esquivel-Chirino, César
Sumaya-Martínez, Teresa
Fregoso-Aguilar, Tomas
Mendoza-Pérez, Jorge
Morales-González, José A.
Investigation on the Protective Effects of Cranberry Against the DNA Damage Induced by Benzo[a]pyrene
title Investigation on the Protective Effects of Cranberry Against the DNA Damage Induced by Benzo[a]pyrene
title_full Investigation on the Protective Effects of Cranberry Against the DNA Damage Induced by Benzo[a]pyrene
title_fullStr Investigation on the Protective Effects of Cranberry Against the DNA Damage Induced by Benzo[a]pyrene
title_full_unstemmed Investigation on the Protective Effects of Cranberry Against the DNA Damage Induced by Benzo[a]pyrene
title_short Investigation on the Protective Effects of Cranberry Against the DNA Damage Induced by Benzo[a]pyrene
title_sort investigation on the protective effects of cranberry against the dna damage induced by benzo[a]pyrene
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268607/
https://www.ncbi.nlm.nih.gov/pubmed/22499190
http://dx.doi.org/10.3390/molecules17044435
work_keys_str_mv AT madrigalsantillaneduardo investigationontheprotectiveeffectsofcranberryagainstthednadamageinducedbybenzoapyrene
AT fragosoantoniosonia investigationontheprotectiveeffectsofcranberryagainstthednadamageinducedbybenzoapyrene
AT valadezvegacarmen investigationontheprotectiveeffectsofcranberryagainstthednadamageinducedbybenzoapyrene
AT solanosolanogloria investigationontheprotectiveeffectsofcranberryagainstthednadamageinducedbybenzoapyrene
AT zunigaperezclara investigationontheprotectiveeffectsofcranberryagainstthednadamageinducedbybenzoapyrene
AT sanchezgutierrezmanuel investigationontheprotectiveeffectsofcranberryagainstthednadamageinducedbybenzoapyrene
AT izquierdovegajeannetta investigationontheprotectiveeffectsofcranberryagainstthednadamageinducedbybenzoapyrene
AT gutierrezsalinasjose investigationontheprotectiveeffectsofcranberryagainstthednadamageinducedbybenzoapyrene
AT esquivelsotojaime investigationontheprotectiveeffectsofcranberryagainstthednadamageinducedbybenzoapyrene
AT esquivelchirinocesar investigationontheprotectiveeffectsofcranberryagainstthednadamageinducedbybenzoapyrene
AT sumayamartinezteresa investigationontheprotectiveeffectsofcranberryagainstthednadamageinducedbybenzoapyrene
AT fregosoaguilartomas investigationontheprotectiveeffectsofcranberryagainstthednadamageinducedbybenzoapyrene
AT mendozaperezjorge investigationontheprotectiveeffectsofcranberryagainstthednadamageinducedbybenzoapyrene
AT moralesgonzalezjosea investigationontheprotectiveeffectsofcranberryagainstthednadamageinducedbybenzoapyrene