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Oligonucleotide-Peptide Conjugates: Solid-Phase Synthesis under Acidic Conditions and Use in ELISA Assays
Here we used solid-phase methods to prepare oligonucleotides carrying fibrin/ filaggrin citrullinated peptides. Post-synthetic conjugation protocols were successfully applied for the synthesis of oligonucleotides carrying small peptides. A stepwise protocol using acid treatment for the final deprote...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268656/ https://www.ncbi.nlm.nih.gov/pubmed/23174899 http://dx.doi.org/10.3390/molecules171213825 |
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author | Aviñó, Anna Gómara, Maria José Malakoutikhah, Morteza Haro, Isabel Eritja, Ramon |
author_facet | Aviñó, Anna Gómara, Maria José Malakoutikhah, Morteza Haro, Isabel Eritja, Ramon |
author_sort | Aviñó, Anna |
collection | PubMed |
description | Here we used solid-phase methods to prepare oligonucleotides carrying fibrin/ filaggrin citrullinated peptides. Post-synthetic conjugation protocols were successfully applied for the synthesis of oligonucleotides carrying small peptides. A stepwise protocol using acid treatment for the final deprotection allowed the preparation of polypyrimidine oligonucleotides carrying longer and arginine-rich peptides. An ELISA-based test using the oligonucleotide-citrullinated peptide conjugates was developed for the detection of anti-citrullinated protein/peptide antibodies in human serum from rheumatoid arthritis patients. |
format | Online Article Text |
id | pubmed-6268656 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62686562018-12-14 Oligonucleotide-Peptide Conjugates: Solid-Phase Synthesis under Acidic Conditions and Use in ELISA Assays Aviñó, Anna Gómara, Maria José Malakoutikhah, Morteza Haro, Isabel Eritja, Ramon Molecules Article Here we used solid-phase methods to prepare oligonucleotides carrying fibrin/ filaggrin citrullinated peptides. Post-synthetic conjugation protocols were successfully applied for the synthesis of oligonucleotides carrying small peptides. A stepwise protocol using acid treatment for the final deprotection allowed the preparation of polypyrimidine oligonucleotides carrying longer and arginine-rich peptides. An ELISA-based test using the oligonucleotide-citrullinated peptide conjugates was developed for the detection of anti-citrullinated protein/peptide antibodies in human serum from rheumatoid arthritis patients. MDPI 2012-11-22 /pmc/articles/PMC6268656/ /pubmed/23174899 http://dx.doi.org/10.3390/molecules171213825 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Aviñó, Anna Gómara, Maria José Malakoutikhah, Morteza Haro, Isabel Eritja, Ramon Oligonucleotide-Peptide Conjugates: Solid-Phase Synthesis under Acidic Conditions and Use in ELISA Assays |
title | Oligonucleotide-Peptide Conjugates: Solid-Phase Synthesis under Acidic Conditions and Use in ELISA Assays |
title_full | Oligonucleotide-Peptide Conjugates: Solid-Phase Synthesis under Acidic Conditions and Use in ELISA Assays |
title_fullStr | Oligonucleotide-Peptide Conjugates: Solid-Phase Synthesis under Acidic Conditions and Use in ELISA Assays |
title_full_unstemmed | Oligonucleotide-Peptide Conjugates: Solid-Phase Synthesis under Acidic Conditions and Use in ELISA Assays |
title_short | Oligonucleotide-Peptide Conjugates: Solid-Phase Synthesis under Acidic Conditions and Use in ELISA Assays |
title_sort | oligonucleotide-peptide conjugates: solid-phase synthesis under acidic conditions and use in elisa assays |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268656/ https://www.ncbi.nlm.nih.gov/pubmed/23174899 http://dx.doi.org/10.3390/molecules171213825 |
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