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Oligonucleotide-Peptide Conjugates: Solid-Phase Synthesis under Acidic Conditions and Use in ELISA Assays

Here we used solid-phase methods to prepare oligonucleotides carrying fibrin/ filaggrin citrullinated peptides. Post-synthetic conjugation protocols were successfully applied for the synthesis of oligonucleotides carrying small peptides. A stepwise protocol using acid treatment for the final deprote...

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Detalles Bibliográficos
Autores principales: Aviñó, Anna, Gómara, Maria José, Malakoutikhah, Morteza, Haro, Isabel, Eritja, Ramon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268656/
https://www.ncbi.nlm.nih.gov/pubmed/23174899
http://dx.doi.org/10.3390/molecules171213825
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author Aviñó, Anna
Gómara, Maria José
Malakoutikhah, Morteza
Haro, Isabel
Eritja, Ramon
author_facet Aviñó, Anna
Gómara, Maria José
Malakoutikhah, Morteza
Haro, Isabel
Eritja, Ramon
author_sort Aviñó, Anna
collection PubMed
description Here we used solid-phase methods to prepare oligonucleotides carrying fibrin/ filaggrin citrullinated peptides. Post-synthetic conjugation protocols were successfully applied for the synthesis of oligonucleotides carrying small peptides. A stepwise protocol using acid treatment for the final deprotection allowed the preparation of polypyrimidine oligonucleotides carrying longer and arginine-rich peptides. An ELISA-based test using the oligonucleotide-citrullinated peptide conjugates was developed for the detection of anti-citrullinated protein/peptide antibodies in human serum from rheumatoid arthritis patients.
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spelling pubmed-62686562018-12-14 Oligonucleotide-Peptide Conjugates: Solid-Phase Synthesis under Acidic Conditions and Use in ELISA Assays Aviñó, Anna Gómara, Maria José Malakoutikhah, Morteza Haro, Isabel Eritja, Ramon Molecules Article Here we used solid-phase methods to prepare oligonucleotides carrying fibrin/ filaggrin citrullinated peptides. Post-synthetic conjugation protocols were successfully applied for the synthesis of oligonucleotides carrying small peptides. A stepwise protocol using acid treatment for the final deprotection allowed the preparation of polypyrimidine oligonucleotides carrying longer and arginine-rich peptides. An ELISA-based test using the oligonucleotide-citrullinated peptide conjugates was developed for the detection of anti-citrullinated protein/peptide antibodies in human serum from rheumatoid arthritis patients. MDPI 2012-11-22 /pmc/articles/PMC6268656/ /pubmed/23174899 http://dx.doi.org/10.3390/molecules171213825 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Aviñó, Anna
Gómara, Maria José
Malakoutikhah, Morteza
Haro, Isabel
Eritja, Ramon
Oligonucleotide-Peptide Conjugates: Solid-Phase Synthesis under Acidic Conditions and Use in ELISA Assays
title Oligonucleotide-Peptide Conjugates: Solid-Phase Synthesis under Acidic Conditions and Use in ELISA Assays
title_full Oligonucleotide-Peptide Conjugates: Solid-Phase Synthesis under Acidic Conditions and Use in ELISA Assays
title_fullStr Oligonucleotide-Peptide Conjugates: Solid-Phase Synthesis under Acidic Conditions and Use in ELISA Assays
title_full_unstemmed Oligonucleotide-Peptide Conjugates: Solid-Phase Synthesis under Acidic Conditions and Use in ELISA Assays
title_short Oligonucleotide-Peptide Conjugates: Solid-Phase Synthesis under Acidic Conditions and Use in ELISA Assays
title_sort oligonucleotide-peptide conjugates: solid-phase synthesis under acidic conditions and use in elisa assays
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268656/
https://www.ncbi.nlm.nih.gov/pubmed/23174899
http://dx.doi.org/10.3390/molecules171213825
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