Cargando…

Repeated Dose 28-Days Oral Toxicity Study of Carica papaya L. Leaf Extract in Sprague Dawley Rats

Carica papaya L. leaves have been used in ethnomedicine for the treatment of fevers and cancers. Despite its benefits, very few studies on their potential toxicity have been described. The aim of the present study was to characterize the chemical composition of the leaf extract from ‘Sekaki’ C. papa...

Descripción completa

Detalles Bibliográficos
Autores principales: Afzan, Adlin, Abdullah, Noor Rain, Halim, Siti Zaleha, Rashid, Badrul Amini, Semail, Raja Hazlini Raja, Abdullah, Noordini, Jantan, Ibrahim, Muhammad, Hussin, Ismail, Zakiah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268730/
https://www.ncbi.nlm.nih.gov/pubmed/22491681
http://dx.doi.org/10.3390/molecules17044326
_version_ 1783376353654996992
author Afzan, Adlin
Abdullah, Noor Rain
Halim, Siti Zaleha
Rashid, Badrul Amini
Semail, Raja Hazlini Raja
Abdullah, Noordini
Jantan, Ibrahim
Muhammad, Hussin
Ismail, Zakiah
author_facet Afzan, Adlin
Abdullah, Noor Rain
Halim, Siti Zaleha
Rashid, Badrul Amini
Semail, Raja Hazlini Raja
Abdullah, Noordini
Jantan, Ibrahim
Muhammad, Hussin
Ismail, Zakiah
author_sort Afzan, Adlin
collection PubMed
description Carica papaya L. leaves have been used in ethnomedicine for the treatment of fevers and cancers. Despite its benefits, very few studies on their potential toxicity have been described. The aim of the present study was to characterize the chemical composition of the leaf extract from ‘Sekaki’ C. papaya cultivar by UPLC-TripleTOF-ESI-MS and to investigate the sub-acute oral toxicity in Sprague Dawley rats at doses of 0.01, 0.14 and 2 g/kg by examining the general behavior, clinical signs, hematological parameters, serum biochemistry and histopathology changes. A total of twelve compounds consisting of one piperidine alkaloid, two organic acids, six malic acid derivatives, and four flavonol glycosides were characterized or tentatively identified in the C. papaya leaf extract. In the sub-acute study, the C. papaya extract did not cause mortality nor were treatment-related changes in body weight, food intake, water level, and hematological parameters observed between treatment and control groups. Some biochemical parameters such as the total protein, HDL-cholesterol, AST, ALT and ALP were elevated in a non-dose dependent manner. Histopathological examination of all organs including liver did not reveal morphological alteration. Other parameters showed non-significant differences between treatment and control groups. The present results suggest that C. papaya leaf extract at a dose up to fourteen times the levels employed in practical use in traditional medicine in Malaysia could be considered safe as a medicinal agent.
format Online
Article
Text
id pubmed-6268730
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-62687302018-12-11 Repeated Dose 28-Days Oral Toxicity Study of Carica papaya L. Leaf Extract in Sprague Dawley Rats Afzan, Adlin Abdullah, Noor Rain Halim, Siti Zaleha Rashid, Badrul Amini Semail, Raja Hazlini Raja Abdullah, Noordini Jantan, Ibrahim Muhammad, Hussin Ismail, Zakiah Molecules Article Carica papaya L. leaves have been used in ethnomedicine for the treatment of fevers and cancers. Despite its benefits, very few studies on their potential toxicity have been described. The aim of the present study was to characterize the chemical composition of the leaf extract from ‘Sekaki’ C. papaya cultivar by UPLC-TripleTOF-ESI-MS and to investigate the sub-acute oral toxicity in Sprague Dawley rats at doses of 0.01, 0.14 and 2 g/kg by examining the general behavior, clinical signs, hematological parameters, serum biochemistry and histopathology changes. A total of twelve compounds consisting of one piperidine alkaloid, two organic acids, six malic acid derivatives, and four flavonol glycosides were characterized or tentatively identified in the C. papaya leaf extract. In the sub-acute study, the C. papaya extract did not cause mortality nor were treatment-related changes in body weight, food intake, water level, and hematological parameters observed between treatment and control groups. Some biochemical parameters such as the total protein, HDL-cholesterol, AST, ALT and ALP were elevated in a non-dose dependent manner. Histopathological examination of all organs including liver did not reveal morphological alteration. Other parameters showed non-significant differences between treatment and control groups. The present results suggest that C. papaya leaf extract at a dose up to fourteen times the levels employed in practical use in traditional medicine in Malaysia could be considered safe as a medicinal agent. MDPI 2012-04-10 /pmc/articles/PMC6268730/ /pubmed/22491681 http://dx.doi.org/10.3390/molecules17044326 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Afzan, Adlin
Abdullah, Noor Rain
Halim, Siti Zaleha
Rashid, Badrul Amini
Semail, Raja Hazlini Raja
Abdullah, Noordini
Jantan, Ibrahim
Muhammad, Hussin
Ismail, Zakiah
Repeated Dose 28-Days Oral Toxicity Study of Carica papaya L. Leaf Extract in Sprague Dawley Rats
title Repeated Dose 28-Days Oral Toxicity Study of Carica papaya L. Leaf Extract in Sprague Dawley Rats
title_full Repeated Dose 28-Days Oral Toxicity Study of Carica papaya L. Leaf Extract in Sprague Dawley Rats
title_fullStr Repeated Dose 28-Days Oral Toxicity Study of Carica papaya L. Leaf Extract in Sprague Dawley Rats
title_full_unstemmed Repeated Dose 28-Days Oral Toxicity Study of Carica papaya L. Leaf Extract in Sprague Dawley Rats
title_short Repeated Dose 28-Days Oral Toxicity Study of Carica papaya L. Leaf Extract in Sprague Dawley Rats
title_sort repeated dose 28-days oral toxicity study of carica papaya l. leaf extract in sprague dawley rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268730/
https://www.ncbi.nlm.nih.gov/pubmed/22491681
http://dx.doi.org/10.3390/molecules17044326
work_keys_str_mv AT afzanadlin repeateddose28daysoraltoxicitystudyofcaricapapayalleafextractinspraguedawleyrats
AT abdullahnoorrain repeateddose28daysoraltoxicitystudyofcaricapapayalleafextractinspraguedawleyrats
AT halimsitizaleha repeateddose28daysoraltoxicitystudyofcaricapapayalleafextractinspraguedawleyrats
AT rashidbadrulamini repeateddose28daysoraltoxicitystudyofcaricapapayalleafextractinspraguedawleyrats
AT semailrajahazliniraja repeateddose28daysoraltoxicitystudyofcaricapapayalleafextractinspraguedawleyrats
AT abdullahnoordini repeateddose28daysoraltoxicitystudyofcaricapapayalleafextractinspraguedawleyrats
AT jantanibrahim repeateddose28daysoraltoxicitystudyofcaricapapayalleafextractinspraguedawleyrats
AT muhammadhussin repeateddose28daysoraltoxicitystudyofcaricapapayalleafextractinspraguedawleyrats
AT ismailzakiah repeateddose28daysoraltoxicitystudyofcaricapapayalleafextractinspraguedawleyrats