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Down Regulation of CIAPIN1 Reverses Multidrug Resistance in Human Breast Cancer Cells by Inhibiting MDR1

Cytokine-induced apoptosis inhibitor 1 (CIAPIN1), initially named anamorsin, a newly indentified antiapoptotic molecule is a downstream effector of the receptor tyrosine kinase-Ras signaling pathway. Current study has revealed that CIAPIN1 may have wide and important functions, especially due to its...

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Autores principales: Lu, Dan, Xiao, Zhibo, Wang, Wenxiu, Xu, Yuqing, Gao, Shujian, Deng, Lili, He, Wen, Yang, Yu, Guo, Xiaofei, Wang, Xuemei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268881/
https://www.ncbi.nlm.nih.gov/pubmed/22717413
http://dx.doi.org/10.3390/molecules17067595
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author Lu, Dan
Xiao, Zhibo
Wang, Wenxiu
Xu, Yuqing
Gao, Shujian
Deng, Lili
He, Wen
Yang, Yu
Guo, Xiaofei
Wang, Xuemei
author_facet Lu, Dan
Xiao, Zhibo
Wang, Wenxiu
Xu, Yuqing
Gao, Shujian
Deng, Lili
He, Wen
Yang, Yu
Guo, Xiaofei
Wang, Xuemei
author_sort Lu, Dan
collection PubMed
description Cytokine-induced apoptosis inhibitor 1 (CIAPIN1), initially named anamorsin, a newly indentified antiapoptotic molecule is a downstream effector of the receptor tyrosine kinase-Ras signaling pathway. Current study has revealed that CIAPIN1 may have wide and important functions, especially due to its close correlations with malignant tumors. However whether or not it is involved in the multi-drug resistance (MDR) process of breast cancer has not been elucidated. To explore the effect of CIAPIN1 on MDR, we examined the expression of P-gp and CIAPIN1 by immunohistochemistry and found there was positive correlation between them. Then we successfully interfered with RNA translation by the infection of siRNA of CIAPIN1 into MCF7/ADM breast cancer cell lines through a lentivirus, and the expression of the target gene was significantly inhibited. After RNAi the drug resistance was reduced significantly and the expression of MDR1mRNA and P-gp in MCF7/ADM cell lines showed a significant decrease. Also the expression of P53 protein increased in a statistically significant way (p ≤ 0.01) after RNAi exposure. In addition, flow cytometry analysis reveals that cell cycle and anti-apoptotic enhancing capability of cells changed after RNAi treatment. These results suggested CIAPIN1 may participate in breast cancer MDR by regulating MDR1 and P53 expression, changing cell cycle and enhancing the anti-apoptotic capability of cells.
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spelling pubmed-62688812018-12-12 Down Regulation of CIAPIN1 Reverses Multidrug Resistance in Human Breast Cancer Cells by Inhibiting MDR1 Lu, Dan Xiao, Zhibo Wang, Wenxiu Xu, Yuqing Gao, Shujian Deng, Lili He, Wen Yang, Yu Guo, Xiaofei Wang, Xuemei Molecules Article Cytokine-induced apoptosis inhibitor 1 (CIAPIN1), initially named anamorsin, a newly indentified antiapoptotic molecule is a downstream effector of the receptor tyrosine kinase-Ras signaling pathway. Current study has revealed that CIAPIN1 may have wide and important functions, especially due to its close correlations with malignant tumors. However whether or not it is involved in the multi-drug resistance (MDR) process of breast cancer has not been elucidated. To explore the effect of CIAPIN1 on MDR, we examined the expression of P-gp and CIAPIN1 by immunohistochemistry and found there was positive correlation between them. Then we successfully interfered with RNA translation by the infection of siRNA of CIAPIN1 into MCF7/ADM breast cancer cell lines through a lentivirus, and the expression of the target gene was significantly inhibited. After RNAi the drug resistance was reduced significantly and the expression of MDR1mRNA and P-gp in MCF7/ADM cell lines showed a significant decrease. Also the expression of P53 protein increased in a statistically significant way (p ≤ 0.01) after RNAi exposure. In addition, flow cytometry analysis reveals that cell cycle and anti-apoptotic enhancing capability of cells changed after RNAi treatment. These results suggested CIAPIN1 may participate in breast cancer MDR by regulating MDR1 and P53 expression, changing cell cycle and enhancing the anti-apoptotic capability of cells. MDPI 2012-06-20 /pmc/articles/PMC6268881/ /pubmed/22717413 http://dx.doi.org/10.3390/molecules17067595 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Lu, Dan
Xiao, Zhibo
Wang, Wenxiu
Xu, Yuqing
Gao, Shujian
Deng, Lili
He, Wen
Yang, Yu
Guo, Xiaofei
Wang, Xuemei
Down Regulation of CIAPIN1 Reverses Multidrug Resistance in Human Breast Cancer Cells by Inhibiting MDR1
title Down Regulation of CIAPIN1 Reverses Multidrug Resistance in Human Breast Cancer Cells by Inhibiting MDR1
title_full Down Regulation of CIAPIN1 Reverses Multidrug Resistance in Human Breast Cancer Cells by Inhibiting MDR1
title_fullStr Down Regulation of CIAPIN1 Reverses Multidrug Resistance in Human Breast Cancer Cells by Inhibiting MDR1
title_full_unstemmed Down Regulation of CIAPIN1 Reverses Multidrug Resistance in Human Breast Cancer Cells by Inhibiting MDR1
title_short Down Regulation of CIAPIN1 Reverses Multidrug Resistance in Human Breast Cancer Cells by Inhibiting MDR1
title_sort down regulation of ciapin1 reverses multidrug resistance in human breast cancer cells by inhibiting mdr1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268881/
https://www.ncbi.nlm.nih.gov/pubmed/22717413
http://dx.doi.org/10.3390/molecules17067595
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