Simple, Rapid and Reliable Preparation of [(11)C]-(+)-α-DTBZ of High Quality for Routine Applications

[(11)C]-(+)-α-DTBZ has been used as a marker of dopaminergic terminal densities in human striatum and expressed in islet beta cells in the pancreas. We aimed to establish a fully automated and simple procedure for the synthesis of [(11)C]-(+)-α-DTBZ for routine applications. [(11)C]-(+)-α-DTBZ was s...

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Detalles Bibliográficos
Autores principales: Zhang, Jinming, Zhang, Xiaojun, Li, Yungang, Tian, Jiahe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268919/
https://www.ncbi.nlm.nih.gov/pubmed/22728363
http://dx.doi.org/10.3390/molecules17066697
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author Zhang, Jinming
Zhang, Xiaojun
Li, Yungang
Tian, Jiahe
author_facet Zhang, Jinming
Zhang, Xiaojun
Li, Yungang
Tian, Jiahe
author_sort Zhang, Jinming
collection PubMed
description [(11)C]-(+)-α-DTBZ has been used as a marker of dopaminergic terminal densities in human striatum and expressed in islet beta cells in the pancreas. We aimed to establish a fully automated and simple procedure for the synthesis of [(11)C]-(+)-α-DTBZ for routine applications. [(11)C]-(+)-α-DTBZ was synthesized from a 9-hydroxy precursor in acetone and potassium hydroxide with [(11)C]-methyl triflate and was purified by solid phase extraction using a Vac tC-18 cartridge. Radiochemical yields based on [(11)C]-methyl triflate (corrected for decay) were 82.3% ± 3.6%, with a specific radioactivity of 60 GBq/μmol. Time elapsed was less than 20 min from end of bombardment to release of the product for quality control.
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spelling pubmed-62689192018-12-12 Simple, Rapid and Reliable Preparation of [(11)C]-(+)-α-DTBZ of High Quality for Routine Applications Zhang, Jinming Zhang, Xiaojun Li, Yungang Tian, Jiahe Molecules Article [(11)C]-(+)-α-DTBZ has been used as a marker of dopaminergic terminal densities in human striatum and expressed in islet beta cells in the pancreas. We aimed to establish a fully automated and simple procedure for the synthesis of [(11)C]-(+)-α-DTBZ for routine applications. [(11)C]-(+)-α-DTBZ was synthesized from a 9-hydroxy precursor in acetone and potassium hydroxide with [(11)C]-methyl triflate and was purified by solid phase extraction using a Vac tC-18 cartridge. Radiochemical yields based on [(11)C]-methyl triflate (corrected for decay) were 82.3% ± 3.6%, with a specific radioactivity of 60 GBq/μmol. Time elapsed was less than 20 min from end of bombardment to release of the product for quality control. MDPI 2012-06-01 /pmc/articles/PMC6268919/ /pubmed/22728363 http://dx.doi.org/10.3390/molecules17066697 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Zhang, Jinming
Zhang, Xiaojun
Li, Yungang
Tian, Jiahe
Simple, Rapid and Reliable Preparation of [(11)C]-(+)-α-DTBZ of High Quality for Routine Applications
title Simple, Rapid and Reliable Preparation of [(11)C]-(+)-α-DTBZ of High Quality for Routine Applications
title_full Simple, Rapid and Reliable Preparation of [(11)C]-(+)-α-DTBZ of High Quality for Routine Applications
title_fullStr Simple, Rapid and Reliable Preparation of [(11)C]-(+)-α-DTBZ of High Quality for Routine Applications
title_full_unstemmed Simple, Rapid and Reliable Preparation of [(11)C]-(+)-α-DTBZ of High Quality for Routine Applications
title_short Simple, Rapid and Reliable Preparation of [(11)C]-(+)-α-DTBZ of High Quality for Routine Applications
title_sort simple, rapid and reliable preparation of [(11)c]-(+)-α-dtbz of high quality for routine applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268919/
https://www.ncbi.nlm.nih.gov/pubmed/22728363
http://dx.doi.org/10.3390/molecules17066697
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