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MDP Up-Regulates the Gene Expression of Type I Interferons in Human Aortic Endothelial Cells
Muramyldipeptide (MDP), the minimum essential structure responsible for the immuno-adjuvant activity of peptidoglycan, is recognized by intracellular nuclear-binding oligomerization domain 2 (NOD2). Here, we obtained evidence that the treatment of human aortic endothelial cells (HAECs) with MDP up-r...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268929/ https://www.ncbi.nlm.nih.gov/pubmed/22447023 http://dx.doi.org/10.3390/molecules17043599 |
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author | Lv, Qingshan Yang, Mei Liu, Xueting Zhou, Lina Xiao, Zhilin Chen, Xiaobin Chen, Meifang Xie, Xiumei Hu, Jinyue |
author_facet | Lv, Qingshan Yang, Mei Liu, Xueting Zhou, Lina Xiao, Zhilin Chen, Xiaobin Chen, Meifang Xie, Xiumei Hu, Jinyue |
author_sort | Lv, Qingshan |
collection | PubMed |
description | Muramyldipeptide (MDP), the minimum essential structure responsible for the immuno-adjuvant activity of peptidoglycan, is recognized by intracellular nuclear-binding oligomerization domain 2 (NOD2). Here, we obtained evidence that the treatment of human aortic endothelial cells (HAECs) with MDP up-regulated the gene expression of type I interferons in a dose- and time-dependent manner. MDP also up-regulated the expression of the receptor NOD2, suggesting that MDP may induce a positive feedback response. The up-regulation of interferons was not dependent on the TNFα signaling, as HAECs did not express TNFα with the stimulation of MDP, and TNFα neutralizing antibody did not decrease the induction of IFNs induced by MDP. RT-PCR results showed that HAECs expressed the gene transcripts of interferon regulatory factor (IRF) 1, 2, 3, 9. The western blot results showed that MDP induced the phosphorylation of IRF3. These results suggested that MDP induced the up-regulation of gene transcript of interferons through the activation of IRF3 signaling pathway. Meanwhile, MDP induced the gene expression of pro-inflammatory cytokines, including IL-1β, IL-8, and MCP-1. Taken together, these results suggested that HAECs may play roles in the anti-infection immune response and in the induction of innate immunity. |
format | Online Article Text |
id | pubmed-6268929 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62689292018-12-11 MDP Up-Regulates the Gene Expression of Type I Interferons in Human Aortic Endothelial Cells Lv, Qingshan Yang, Mei Liu, Xueting Zhou, Lina Xiao, Zhilin Chen, Xiaobin Chen, Meifang Xie, Xiumei Hu, Jinyue Molecules Article Muramyldipeptide (MDP), the minimum essential structure responsible for the immuno-adjuvant activity of peptidoglycan, is recognized by intracellular nuclear-binding oligomerization domain 2 (NOD2). Here, we obtained evidence that the treatment of human aortic endothelial cells (HAECs) with MDP up-regulated the gene expression of type I interferons in a dose- and time-dependent manner. MDP also up-regulated the expression of the receptor NOD2, suggesting that MDP may induce a positive feedback response. The up-regulation of interferons was not dependent on the TNFα signaling, as HAECs did not express TNFα with the stimulation of MDP, and TNFα neutralizing antibody did not decrease the induction of IFNs induced by MDP. RT-PCR results showed that HAECs expressed the gene transcripts of interferon regulatory factor (IRF) 1, 2, 3, 9. The western blot results showed that MDP induced the phosphorylation of IRF3. These results suggested that MDP induced the up-regulation of gene transcript of interferons through the activation of IRF3 signaling pathway. Meanwhile, MDP induced the gene expression of pro-inflammatory cytokines, including IL-1β, IL-8, and MCP-1. Taken together, these results suggested that HAECs may play roles in the anti-infection immune response and in the induction of innate immunity. MDPI 2012-03-23 /pmc/articles/PMC6268929/ /pubmed/22447023 http://dx.doi.org/10.3390/molecules17043599 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Lv, Qingshan Yang, Mei Liu, Xueting Zhou, Lina Xiao, Zhilin Chen, Xiaobin Chen, Meifang Xie, Xiumei Hu, Jinyue MDP Up-Regulates the Gene Expression of Type I Interferons in Human Aortic Endothelial Cells |
title | MDP Up-Regulates the Gene Expression of Type I Interferons in Human Aortic Endothelial Cells |
title_full | MDP Up-Regulates the Gene Expression of Type I Interferons in Human Aortic Endothelial Cells |
title_fullStr | MDP Up-Regulates the Gene Expression of Type I Interferons in Human Aortic Endothelial Cells |
title_full_unstemmed | MDP Up-Regulates the Gene Expression of Type I Interferons in Human Aortic Endothelial Cells |
title_short | MDP Up-Regulates the Gene Expression of Type I Interferons in Human Aortic Endothelial Cells |
title_sort | mdp up-regulates the gene expression of type i interferons in human aortic endothelial cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268929/ https://www.ncbi.nlm.nih.gov/pubmed/22447023 http://dx.doi.org/10.3390/molecules17043599 |
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