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Coupling Two Different Nucleic Acid Circuits in an Enzyme-Free Amplifier

DNA circuits have proven to be useful amplifiers for diagnostic applications, in part because of their modularity and programmability. In order to determine whether different circuits could be modularly stacked, we used a catalytic hairpin assembly (CHA) circuit to initiate a hybridization chain rea...

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Detalles Bibliográficos
Autores principales: Jiang, Yu, Li, Bingling, Chen, Xi, Ellington, Andrew D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268946/
https://www.ncbi.nlm.nih.gov/pubmed/23132138
http://dx.doi.org/10.3390/molecules171113211
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author Jiang, Yu
Li, Bingling
Chen, Xi
Ellington, Andrew D.
author_facet Jiang, Yu
Li, Bingling
Chen, Xi
Ellington, Andrew D.
author_sort Jiang, Yu
collection PubMed
description DNA circuits have proven to be useful amplifiers for diagnostic applications, in part because of their modularity and programmability. In order to determine whether different circuits could be modularly stacked, we used a catalytic hairpin assembly (CHA) circuit to initiate a hybridization chain reaction (HCR) circuit. In response to an input nucleic acid sequence, the CHA reaction accumulates immobilized duplexes and HCR elongates these duplexes. With fluorescein as a reporter each of these processes yielded 10-fold signal amplification in a convenient 96-well format. The modular circuit connections also allowed the output reporter to be readily modified to a G-quadruplex-DNAzyme that yielded a fluorescent signal.
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spelling pubmed-62689462018-12-13 Coupling Two Different Nucleic Acid Circuits in an Enzyme-Free Amplifier Jiang, Yu Li, Bingling Chen, Xi Ellington, Andrew D. Molecules Article DNA circuits have proven to be useful amplifiers for diagnostic applications, in part because of their modularity and programmability. In order to determine whether different circuits could be modularly stacked, we used a catalytic hairpin assembly (CHA) circuit to initiate a hybridization chain reaction (HCR) circuit. In response to an input nucleic acid sequence, the CHA reaction accumulates immobilized duplexes and HCR elongates these duplexes. With fluorescein as a reporter each of these processes yielded 10-fold signal amplification in a convenient 96-well format. The modular circuit connections also allowed the output reporter to be readily modified to a G-quadruplex-DNAzyme that yielded a fluorescent signal. MDPI 2012-11-06 /pmc/articles/PMC6268946/ /pubmed/23132138 http://dx.doi.org/10.3390/molecules171113211 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Jiang, Yu
Li, Bingling
Chen, Xi
Ellington, Andrew D.
Coupling Two Different Nucleic Acid Circuits in an Enzyme-Free Amplifier
title Coupling Two Different Nucleic Acid Circuits in an Enzyme-Free Amplifier
title_full Coupling Two Different Nucleic Acid Circuits in an Enzyme-Free Amplifier
title_fullStr Coupling Two Different Nucleic Acid Circuits in an Enzyme-Free Amplifier
title_full_unstemmed Coupling Two Different Nucleic Acid Circuits in an Enzyme-Free Amplifier
title_short Coupling Two Different Nucleic Acid Circuits in an Enzyme-Free Amplifier
title_sort coupling two different nucleic acid circuits in an enzyme-free amplifier
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268946/
https://www.ncbi.nlm.nih.gov/pubmed/23132138
http://dx.doi.org/10.3390/molecules171113211
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