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Coupling Two Different Nucleic Acid Circuits in an Enzyme-Free Amplifier
DNA circuits have proven to be useful amplifiers for diagnostic applications, in part because of their modularity and programmability. In order to determine whether different circuits could be modularly stacked, we used a catalytic hairpin assembly (CHA) circuit to initiate a hybridization chain rea...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268946/ https://www.ncbi.nlm.nih.gov/pubmed/23132138 http://dx.doi.org/10.3390/molecules171113211 |
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author | Jiang, Yu Li, Bingling Chen, Xi Ellington, Andrew D. |
author_facet | Jiang, Yu Li, Bingling Chen, Xi Ellington, Andrew D. |
author_sort | Jiang, Yu |
collection | PubMed |
description | DNA circuits have proven to be useful amplifiers for diagnostic applications, in part because of their modularity and programmability. In order to determine whether different circuits could be modularly stacked, we used a catalytic hairpin assembly (CHA) circuit to initiate a hybridization chain reaction (HCR) circuit. In response to an input nucleic acid sequence, the CHA reaction accumulates immobilized duplexes and HCR elongates these duplexes. With fluorescein as a reporter each of these processes yielded 10-fold signal amplification in a convenient 96-well format. The modular circuit connections also allowed the output reporter to be readily modified to a G-quadruplex-DNAzyme that yielded a fluorescent signal. |
format | Online Article Text |
id | pubmed-6268946 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62689462018-12-13 Coupling Two Different Nucleic Acid Circuits in an Enzyme-Free Amplifier Jiang, Yu Li, Bingling Chen, Xi Ellington, Andrew D. Molecules Article DNA circuits have proven to be useful amplifiers for diagnostic applications, in part because of their modularity and programmability. In order to determine whether different circuits could be modularly stacked, we used a catalytic hairpin assembly (CHA) circuit to initiate a hybridization chain reaction (HCR) circuit. In response to an input nucleic acid sequence, the CHA reaction accumulates immobilized duplexes and HCR elongates these duplexes. With fluorescein as a reporter each of these processes yielded 10-fold signal amplification in a convenient 96-well format. The modular circuit connections also allowed the output reporter to be readily modified to a G-quadruplex-DNAzyme that yielded a fluorescent signal. MDPI 2012-11-06 /pmc/articles/PMC6268946/ /pubmed/23132138 http://dx.doi.org/10.3390/molecules171113211 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Jiang, Yu Li, Bingling Chen, Xi Ellington, Andrew D. Coupling Two Different Nucleic Acid Circuits in an Enzyme-Free Amplifier |
title | Coupling Two Different Nucleic Acid Circuits in an Enzyme-Free Amplifier |
title_full | Coupling Two Different Nucleic Acid Circuits in an Enzyme-Free Amplifier |
title_fullStr | Coupling Two Different Nucleic Acid Circuits in an Enzyme-Free Amplifier |
title_full_unstemmed | Coupling Two Different Nucleic Acid Circuits in an Enzyme-Free Amplifier |
title_short | Coupling Two Different Nucleic Acid Circuits in an Enzyme-Free Amplifier |
title_sort | coupling two different nucleic acid circuits in an enzyme-free amplifier |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268946/ https://www.ncbi.nlm.nih.gov/pubmed/23132138 http://dx.doi.org/10.3390/molecules171113211 |
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