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Bio-Functional Constituents from the Stems of Liriodendron tulipifera
Four known compounds have been isolated from the stems of Liriodendron tulipifera, and the structures of these pure constituents were determined using spectroscopic analysis. Isolated compounds were screened for free radical scavenging ability, metal chelating power assay and ferric reducing antioxi...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268983/ https://www.ncbi.nlm.nih.gov/pubmed/22491683 http://dx.doi.org/10.3390/molecules17044357 |
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author | Chiu, Chien-Chih Chou, Han-Lin Wu, Pei-Fang Chen, Hsin-Liang Wang, Hui-Min Chen, Chung-Yi |
author_facet | Chiu, Chien-Chih Chou, Han-Lin Wu, Pei-Fang Chen, Hsin-Liang Wang, Hui-Min Chen, Chung-Yi |
author_sort | Chiu, Chien-Chih |
collection | PubMed |
description | Four known compounds have been isolated from the stems of Liriodendron tulipifera, and the structures of these pure constituents were determined using spectroscopic analysis. Isolated compounds were screened for free radical scavenging ability, metal chelating power assay and ferric reducing antioxidant power assay (FRAP). The anti-tyrosinase effects of L. tulipifera compounds were calculated the inhibition of hydroxylation of L-tyrosine to L-dopa according to an in vitro mushroom tyrosinase assay. The study also examined the bio-effects of the four compounds on the human melanoma A375.S2, and showed that liriodenine (1) and (-)-norglaucine (4) significantly inhibited the proliferation of melanoma cells in the cell viability assay. Wound healing results indicated that liriodenine (1), (-)-glaucine (3) and (-)-norglaucine (4) exerted anti-migration potential. Interestingly, (-)-glaucine (3), neither liriodenine (1) nor (-)-norglaucine (4) showed promising anti-migration potential without inducing significant cytotoxicity. Furthermore, a dramatically increased level of intracellular reactive oxygen species (ROS) was detected from (-)-glaucine (3). The cell cycle assessment demonstrated a moderate G2/M accumulation by (-)-glaucine (3). The above results revealed the anti-cancer effects of L. tulipifera compounds, especially on the anti-migration ability indicating the promising chemopreventive agents to human skin melanoma cells. |
format | Online Article Text |
id | pubmed-6268983 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62689832018-12-11 Bio-Functional Constituents from the Stems of Liriodendron tulipifera Chiu, Chien-Chih Chou, Han-Lin Wu, Pei-Fang Chen, Hsin-Liang Wang, Hui-Min Chen, Chung-Yi Molecules Article Four known compounds have been isolated from the stems of Liriodendron tulipifera, and the structures of these pure constituents were determined using spectroscopic analysis. Isolated compounds were screened for free radical scavenging ability, metal chelating power assay and ferric reducing antioxidant power assay (FRAP). The anti-tyrosinase effects of L. tulipifera compounds were calculated the inhibition of hydroxylation of L-tyrosine to L-dopa according to an in vitro mushroom tyrosinase assay. The study also examined the bio-effects of the four compounds on the human melanoma A375.S2, and showed that liriodenine (1) and (-)-norglaucine (4) significantly inhibited the proliferation of melanoma cells in the cell viability assay. Wound healing results indicated that liriodenine (1), (-)-glaucine (3) and (-)-norglaucine (4) exerted anti-migration potential. Interestingly, (-)-glaucine (3), neither liriodenine (1) nor (-)-norglaucine (4) showed promising anti-migration potential without inducing significant cytotoxicity. Furthermore, a dramatically increased level of intracellular reactive oxygen species (ROS) was detected from (-)-glaucine (3). The cell cycle assessment demonstrated a moderate G2/M accumulation by (-)-glaucine (3). The above results revealed the anti-cancer effects of L. tulipifera compounds, especially on the anti-migration ability indicating the promising chemopreventive agents to human skin melanoma cells. MDPI 2012-04-10 /pmc/articles/PMC6268983/ /pubmed/22491683 http://dx.doi.org/10.3390/molecules17044357 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Chiu, Chien-Chih Chou, Han-Lin Wu, Pei-Fang Chen, Hsin-Liang Wang, Hui-Min Chen, Chung-Yi Bio-Functional Constituents from the Stems of Liriodendron tulipifera |
title | Bio-Functional Constituents from the Stems of Liriodendron tulipifera |
title_full | Bio-Functional Constituents from the Stems of Liriodendron tulipifera |
title_fullStr | Bio-Functional Constituents from the Stems of Liriodendron tulipifera |
title_full_unstemmed | Bio-Functional Constituents from the Stems of Liriodendron tulipifera |
title_short | Bio-Functional Constituents from the Stems of Liriodendron tulipifera |
title_sort | bio-functional constituents from the stems of liriodendron tulipifera |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268983/ https://www.ncbi.nlm.nih.gov/pubmed/22491683 http://dx.doi.org/10.3390/molecules17044357 |
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