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Regulation of Inflammatory Cytokines in Lipopolysaccharide-Stimulated RAW 264.7 Murine Macrophage by 7-O-Methyl-naringenin

7-O-Methylnaringenin, extracted from Rhododendron speciferum, belongs to the flavanone class of polyphenols. In the present study, we investigated the anti-inflammatory effects of 7-O-methylnaringenin on cytokine production by lipopoly-saccharide (LPS)-stimulated RAW 264.7 macrophages in vitro. The...

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Autores principales: Soromou, Lanan Wassy, Zhang, Zhichao, Li, Rongtao, Chen, Na, Guo, Weixiao, Huo, Meixia, Guan, Shuang, Lu, Jing, Deng, Xuming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269002/
https://www.ncbi.nlm.nih.gov/pubmed/22441335
http://dx.doi.org/10.3390/molecules17033574
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author Soromou, Lanan Wassy
Zhang, Zhichao
Li, Rongtao
Chen, Na
Guo, Weixiao
Huo, Meixia
Guan, Shuang
Lu, Jing
Deng, Xuming
author_facet Soromou, Lanan Wassy
Zhang, Zhichao
Li, Rongtao
Chen, Na
Guo, Weixiao
Huo, Meixia
Guan, Shuang
Lu, Jing
Deng, Xuming
author_sort Soromou, Lanan Wassy
collection PubMed
description 7-O-Methylnaringenin, extracted from Rhododendron speciferum, belongs to the flavanone class of polyphenols. In the present study, we investigated the anti-inflammatory effects of 7-O-methylnaringenin on cytokine production by lipopoly-saccharide (LPS)-stimulated RAW 264.7 macrophages in vitro. The results showed that pretreatment with 10, 20 or 40 μg/mL of 7-O-methylnaringenin could downregulate tumour necrosis factor (TNF-α), interleukin (IL-6) and interleukin (IL-1β) in a dose-dependent manner. Furthermore, we investigated the signal transduction mechanisms to determine how 7-O-methylnaringenin affects RAW 264.7 macrophages. The activation of mitogen-activated protein kinases (MAPK) and IκBα were measured by Western blotting. The data showed that 7-O-methylnaringenin could downregulate LPS-induced levels of phosphorylation of ERK1/2, JNK and IκBα. These observations indicated that 7-O-methylnaringenin modulated inflammatory cytokine responses by blocking NF-қB, ERK1/2 and JNK/MAPKs activation.
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spelling pubmed-62690022018-12-20 Regulation of Inflammatory Cytokines in Lipopolysaccharide-Stimulated RAW 264.7 Murine Macrophage by 7-O-Methyl-naringenin Soromou, Lanan Wassy Zhang, Zhichao Li, Rongtao Chen, Na Guo, Weixiao Huo, Meixia Guan, Shuang Lu, Jing Deng, Xuming Molecules Article 7-O-Methylnaringenin, extracted from Rhododendron speciferum, belongs to the flavanone class of polyphenols. In the present study, we investigated the anti-inflammatory effects of 7-O-methylnaringenin on cytokine production by lipopoly-saccharide (LPS)-stimulated RAW 264.7 macrophages in vitro. The results showed that pretreatment with 10, 20 or 40 μg/mL of 7-O-methylnaringenin could downregulate tumour necrosis factor (TNF-α), interleukin (IL-6) and interleukin (IL-1β) in a dose-dependent manner. Furthermore, we investigated the signal transduction mechanisms to determine how 7-O-methylnaringenin affects RAW 264.7 macrophages. The activation of mitogen-activated protein kinases (MAPK) and IκBα were measured by Western blotting. The data showed that 7-O-methylnaringenin could downregulate LPS-induced levels of phosphorylation of ERK1/2, JNK and IκBα. These observations indicated that 7-O-methylnaringenin modulated inflammatory cytokine responses by blocking NF-қB, ERK1/2 and JNK/MAPKs activation. MDPI 2012-03-22 /pmc/articles/PMC6269002/ /pubmed/22441335 http://dx.doi.org/10.3390/molecules17033574 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Soromou, Lanan Wassy
Zhang, Zhichao
Li, Rongtao
Chen, Na
Guo, Weixiao
Huo, Meixia
Guan, Shuang
Lu, Jing
Deng, Xuming
Regulation of Inflammatory Cytokines in Lipopolysaccharide-Stimulated RAW 264.7 Murine Macrophage by 7-O-Methyl-naringenin
title Regulation of Inflammatory Cytokines in Lipopolysaccharide-Stimulated RAW 264.7 Murine Macrophage by 7-O-Methyl-naringenin
title_full Regulation of Inflammatory Cytokines in Lipopolysaccharide-Stimulated RAW 264.7 Murine Macrophage by 7-O-Methyl-naringenin
title_fullStr Regulation of Inflammatory Cytokines in Lipopolysaccharide-Stimulated RAW 264.7 Murine Macrophage by 7-O-Methyl-naringenin
title_full_unstemmed Regulation of Inflammatory Cytokines in Lipopolysaccharide-Stimulated RAW 264.7 Murine Macrophage by 7-O-Methyl-naringenin
title_short Regulation of Inflammatory Cytokines in Lipopolysaccharide-Stimulated RAW 264.7 Murine Macrophage by 7-O-Methyl-naringenin
title_sort regulation of inflammatory cytokines in lipopolysaccharide-stimulated raw 264.7 murine macrophage by 7-o-methyl-naringenin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269002/
https://www.ncbi.nlm.nih.gov/pubmed/22441335
http://dx.doi.org/10.3390/molecules17033574
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