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Activity-Guided Isolation of Antioxidant Compounds from Rhizophora apiculata

Rhizophora apiculata (R. apiculata) contains an abundance of biologically active compounds due its special salt-tolerant living surroundings. In this study, the total phenolic content and antioxidant activities of various extract and fractions of stem of R. apiculata were investigated. Results indic...

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Detalles Bibliográficos
Autores principales: Gao, Mingzhe, Xiao, Hongbin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269029/
https://www.ncbi.nlm.nih.gov/pubmed/22955456
http://dx.doi.org/10.3390/molecules170910675
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author Gao, Mingzhe
Xiao, Hongbin
author_facet Gao, Mingzhe
Xiao, Hongbin
author_sort Gao, Mingzhe
collection PubMed
description Rhizophora apiculata (R. apiculata) contains an abundance of biologically active compounds due its special salt-tolerant living surroundings. In this study, the total phenolic content and antioxidant activities of various extract and fractions of stem of R. apiculata were investigated. Results indicated that butanol fraction possesses the highest total phenolic content (181.84 mg/g GAE/g dry extract) with strongest antioxidant abilities. Following in vitro antioxidant activity-guided phytochemical separation procedures, lyoniresinol-3α-O-β-arabinopyranoside (1), lyoniresinol-3α-O-β-rhamnoside (2), and afzelechin-3-O-L-rhamno-pyranoside (3) were separated from the butanol fraction. These compounds showed more noticeable antioxidant activity than a BHT standard in the DPPH, ABTS and hydroxyl radical scavenging assays. HPLC analysis results showed that among different plant parts, the highest content of 1–3 was located in the bark (0.068%, 0.066% and 0.011%, respectively). The results imply that the R. apiculata might be a potential source of natural antioxidants and 1–3 are antioxidant ingredients in R. apiculata.
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spelling pubmed-62690292018-12-12 Activity-Guided Isolation of Antioxidant Compounds from Rhizophora apiculata Gao, Mingzhe Xiao, Hongbin Molecules Article Rhizophora apiculata (R. apiculata) contains an abundance of biologically active compounds due its special salt-tolerant living surroundings. In this study, the total phenolic content and antioxidant activities of various extract and fractions of stem of R. apiculata were investigated. Results indicated that butanol fraction possesses the highest total phenolic content (181.84 mg/g GAE/g dry extract) with strongest antioxidant abilities. Following in vitro antioxidant activity-guided phytochemical separation procedures, lyoniresinol-3α-O-β-arabinopyranoside (1), lyoniresinol-3α-O-β-rhamnoside (2), and afzelechin-3-O-L-rhamno-pyranoside (3) were separated from the butanol fraction. These compounds showed more noticeable antioxidant activity than a BHT standard in the DPPH, ABTS and hydroxyl radical scavenging assays. HPLC analysis results showed that among different plant parts, the highest content of 1–3 was located in the bark (0.068%, 0.066% and 0.011%, respectively). The results imply that the R. apiculata might be a potential source of natural antioxidants and 1–3 are antioxidant ingredients in R. apiculata. MDPI 2012-09-06 /pmc/articles/PMC6269029/ /pubmed/22955456 http://dx.doi.org/10.3390/molecules170910675 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Gao, Mingzhe
Xiao, Hongbin
Activity-Guided Isolation of Antioxidant Compounds from Rhizophora apiculata
title Activity-Guided Isolation of Antioxidant Compounds from Rhizophora apiculata
title_full Activity-Guided Isolation of Antioxidant Compounds from Rhizophora apiculata
title_fullStr Activity-Guided Isolation of Antioxidant Compounds from Rhizophora apiculata
title_full_unstemmed Activity-Guided Isolation of Antioxidant Compounds from Rhizophora apiculata
title_short Activity-Guided Isolation of Antioxidant Compounds from Rhizophora apiculata
title_sort activity-guided isolation of antioxidant compounds from rhizophora apiculata
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269029/
https://www.ncbi.nlm.nih.gov/pubmed/22955456
http://dx.doi.org/10.3390/molecules170910675
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