Separation and Identification of Highly Fluorescent Compounds Derived from trans-Resveratrol in the Leaves of Vitis vinifera Infected by Plasmopara viticola

A method for identification of highly fluorescent compounds in vine leaves infected by Plasmopara viticola was developed using reversed phase liquid chromatography with simultaneous diode array and fluorometric detection. Fluorescent compounds were extracted from leaves with a methanol-water mixture...

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Autores principales: Tříska, Jan, Vrchotová, Naděžda, Olejníčková, Julie, Jílek, Rudolf, Sotolář, Radek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269111/
https://www.ncbi.nlm.nih.gov/pubmed/22395406
http://dx.doi.org/10.3390/molecules17032773
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author Tříska, Jan
Vrchotová, Naděžda
Olejníčková, Julie
Jílek, Rudolf
Sotolář, Radek
author_facet Tříska, Jan
Vrchotová, Naděžda
Olejníčková, Julie
Jílek, Rudolf
Sotolář, Radek
author_sort Tříska, Jan
collection PubMed
description A method for identification of highly fluorescent compounds in vine leaves infected by Plasmopara viticola was developed using reversed phase liquid chromatography with simultaneous diode array and fluorometric detection. Fluorescent compounds were extracted from leaves with a methanol-water mixture (70:30). Separation by HPLC was performed using a C(18) column and gradient elution with water-acetonitrile mixtures (20–80% of acetonitrile). The main unknown fluorescent compound was identified by line spectral comparison with a standard obtained by UV photoisomerization of trans-resveratrol glucoside, and its structure was confirmed by liquid chromatography-mass spectrometry. Identification and structural elucidation of the fluorescent compound in the leaves of Vitis vinifera allows early detection of Plasmopara viticola invasion.
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spelling pubmed-62691112018-12-20 Separation and Identification of Highly Fluorescent Compounds Derived from trans-Resveratrol in the Leaves of Vitis vinifera Infected by Plasmopara viticola Tříska, Jan Vrchotová, Naděžda Olejníčková, Julie Jílek, Rudolf Sotolář, Radek Molecules Article A method for identification of highly fluorescent compounds in vine leaves infected by Plasmopara viticola was developed using reversed phase liquid chromatography with simultaneous diode array and fluorometric detection. Fluorescent compounds were extracted from leaves with a methanol-water mixture (70:30). Separation by HPLC was performed using a C(18) column and gradient elution with water-acetonitrile mixtures (20–80% of acetonitrile). The main unknown fluorescent compound was identified by line spectral comparison with a standard obtained by UV photoisomerization of trans-resveratrol glucoside, and its structure was confirmed by liquid chromatography-mass spectrometry. Identification and structural elucidation of the fluorescent compound in the leaves of Vitis vinifera allows early detection of Plasmopara viticola invasion. MDPI 2012-03-06 /pmc/articles/PMC6269111/ /pubmed/22395406 http://dx.doi.org/10.3390/molecules17032773 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Tříska, Jan
Vrchotová, Naděžda
Olejníčková, Julie
Jílek, Rudolf
Sotolář, Radek
Separation and Identification of Highly Fluorescent Compounds Derived from trans-Resveratrol in the Leaves of Vitis vinifera Infected by Plasmopara viticola
title Separation and Identification of Highly Fluorescent Compounds Derived from trans-Resveratrol in the Leaves of Vitis vinifera Infected by Plasmopara viticola
title_full Separation and Identification of Highly Fluorescent Compounds Derived from trans-Resveratrol in the Leaves of Vitis vinifera Infected by Plasmopara viticola
title_fullStr Separation and Identification of Highly Fluorescent Compounds Derived from trans-Resveratrol in the Leaves of Vitis vinifera Infected by Plasmopara viticola
title_full_unstemmed Separation and Identification of Highly Fluorescent Compounds Derived from trans-Resveratrol in the Leaves of Vitis vinifera Infected by Plasmopara viticola
title_short Separation and Identification of Highly Fluorescent Compounds Derived from trans-Resveratrol in the Leaves of Vitis vinifera Infected by Plasmopara viticola
title_sort separation and identification of highly fluorescent compounds derived from trans-resveratrol in the leaves of vitis vinifera infected by plasmopara viticola
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269111/
https://www.ncbi.nlm.nih.gov/pubmed/22395406
http://dx.doi.org/10.3390/molecules17032773
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