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Cyanidin-3-glucoside enhances mitochondrial function and biogenesis in a human hepatocyte cell line
Mitochondrial dysfunction has been identified as one of the primary factors contributing to liver diseases. Pathways that control mitochondrial biogenesis are potential therapeutic targets for the amelioration of hepatocyte dysfunction and liver disease. Research on natural pharmacological agents th...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269359/ https://www.ncbi.nlm.nih.gov/pubmed/30155610 http://dx.doi.org/10.1007/s10616-018-0242-4 |
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author | Mogalli, Rashad Matsukawa, Toshiya Shimomura, Osamu Isoda, Hiroko Ohkohchi, Nobuhiro |
author_facet | Mogalli, Rashad Matsukawa, Toshiya Shimomura, Osamu Isoda, Hiroko Ohkohchi, Nobuhiro |
author_sort | Mogalli, Rashad |
collection | PubMed |
description | Mitochondrial dysfunction has been identified as one of the primary factors contributing to liver diseases. Pathways that control mitochondrial biogenesis are potential therapeutic targets for the amelioration of hepatocyte dysfunction and liver disease. Research on natural pharmacological agents that ameliorate liver diseases has intensified over the last two decades. Cyanidin-3-glucoside (Cy3g), a dietary flavonoid compound extracted from a wide variety of fruits and vegetables, reportedly has several beneficial health effects. In this study, we used an adult human hepatoma cell line (HuH7) to investigate the effects of the Cy3g polyphenolic compound on mitochondrial function and biogenesis in vitro. An increase in intracellular mitochondrial reductase levels was observed after treatment with Cy3g, but cytotoxicity was not induced. In addition, mitochondrial membrane potential and ATP production were increased following Cy3g treatment. Cy3g treatment also resulted in a dose- and time-dependent upregulation of the gene expression of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), a transcription factor considered a master regulator of mitochondrial biogenesis and metabolism. Additionally, the expression of sirtuin 1 (SIRT1), which plays a key role in deacetylating PGC-1α, was also increased in a dose- and time-dependent manner. Cy3g treatment also increased the expression of downstream PGC-1α genes, nuclear respiratory factor 1 and mitochondrial transcription factor A (TFAM). Our results suggest that Cy3g has potential as a hepatoprotective therapeutic agent that enhances mitochondrial function and biogenesis in hepatocytes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s10616-018-0242-4) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6269359 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-62693592018-12-11 Cyanidin-3-glucoside enhances mitochondrial function and biogenesis in a human hepatocyte cell line Mogalli, Rashad Matsukawa, Toshiya Shimomura, Osamu Isoda, Hiroko Ohkohchi, Nobuhiro Cytotechnology Article Mitochondrial dysfunction has been identified as one of the primary factors contributing to liver diseases. Pathways that control mitochondrial biogenesis are potential therapeutic targets for the amelioration of hepatocyte dysfunction and liver disease. Research on natural pharmacological agents that ameliorate liver diseases has intensified over the last two decades. Cyanidin-3-glucoside (Cy3g), a dietary flavonoid compound extracted from a wide variety of fruits and vegetables, reportedly has several beneficial health effects. In this study, we used an adult human hepatoma cell line (HuH7) to investigate the effects of the Cy3g polyphenolic compound on mitochondrial function and biogenesis in vitro. An increase in intracellular mitochondrial reductase levels was observed after treatment with Cy3g, but cytotoxicity was not induced. In addition, mitochondrial membrane potential and ATP production were increased following Cy3g treatment. Cy3g treatment also resulted in a dose- and time-dependent upregulation of the gene expression of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), a transcription factor considered a master regulator of mitochondrial biogenesis and metabolism. Additionally, the expression of sirtuin 1 (SIRT1), which plays a key role in deacetylating PGC-1α, was also increased in a dose- and time-dependent manner. Cy3g treatment also increased the expression of downstream PGC-1α genes, nuclear respiratory factor 1 and mitochondrial transcription factor A (TFAM). Our results suggest that Cy3g has potential as a hepatoprotective therapeutic agent that enhances mitochondrial function and biogenesis in hepatocytes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s10616-018-0242-4) contains supplementary material, which is available to authorized users. Springer Netherlands 2018-08-28 2018-12 /pmc/articles/PMC6269359/ /pubmed/30155610 http://dx.doi.org/10.1007/s10616-018-0242-4 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Article Mogalli, Rashad Matsukawa, Toshiya Shimomura, Osamu Isoda, Hiroko Ohkohchi, Nobuhiro Cyanidin-3-glucoside enhances mitochondrial function and biogenesis in a human hepatocyte cell line |
title | Cyanidin-3-glucoside enhances mitochondrial function and biogenesis in a human hepatocyte cell line |
title_full | Cyanidin-3-glucoside enhances mitochondrial function and biogenesis in a human hepatocyte cell line |
title_fullStr | Cyanidin-3-glucoside enhances mitochondrial function and biogenesis in a human hepatocyte cell line |
title_full_unstemmed | Cyanidin-3-glucoside enhances mitochondrial function and biogenesis in a human hepatocyte cell line |
title_short | Cyanidin-3-glucoside enhances mitochondrial function and biogenesis in a human hepatocyte cell line |
title_sort | cyanidin-3-glucoside enhances mitochondrial function and biogenesis in a human hepatocyte cell line |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269359/ https://www.ncbi.nlm.nih.gov/pubmed/30155610 http://dx.doi.org/10.1007/s10616-018-0242-4 |
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